CHARACTERIZATION OF MICROBIAL POPULATIONS OF LAKE VAN BY 16S METAGENOMICS STUDY

Nilgün Poyraz; Mehmet Mutlu

doi:10.18036/estubtdc.680795

CHARACTERIZATION OF MICROBIAL POPULATIONS OF LAKE VAN BY 16S METAGENOMICS STUDY

Abstract

Van Lake, located in Eastern Anatolia Region, is the largest lake in Turkey. This lake features alkaline and saline soda lake. Since there are few studies focused on microbial diversity of alkaline environments, it is very important to reveal the diversity of microorganisms of Van Lake because of the wide range of biotechnological uses of these microorganisms and their ecological roles in lake ecosystem. Next generation sequencing (NGS) allow us to sequence DNA and RNA much more quickly and cheaply than the previously used Sanger sequencing for the rapid analysis of the composition and diversity of microbial communities in several habitats. We applied the high throughput techniques of NGS to the metagenomics study of Van Lake, by assessing its PCR amplicon of 16S rDNA sequences (V3–V4 regions). The analyses revealed Proteobacteria, Actinobacteria and Verrucomicrobia phyla were most abundant. Archaea members were represented by Euryarchaeota phylum. Results concluded that Bacteria domain were dominant in Van Lake.

Keywords

Van Lake,16S rRNA,Microbial diversity

References

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Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Nilgün Poyraz ^*
0000-0002-5861-7922
Türkiye

Mehmet Mutlu
0000-0002-9404-6389
Türkiye

Publication Date

January 31, 2020

Submission Date

January 27, 2020

Acceptance Date

January 29, 2020

Published in Issue

Year 2020 Volume: 9 Number: 1

DOI

https://doi.org/10.18036/estubtdc.680795

IZ

https://izlik.org/JA65UJ25KG

APA

Poyraz, N., & Mutlu, M. (2020). CHARACTERIZATION OF MICROBIAL POPULATIONS OF LAKE VAN BY 16S METAGENOMICS STUDY. Eskişehir Teknik Üniversitesi Bilim Ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji, 9(1), 80-88. https://doi.org/10.18036/estubtdc.680795

AMA

1.Poyraz N, Mutlu M. CHARACTERIZATION OF MICROBIAL POPULATIONS OF LAKE VAN BY 16S METAGENOMICS STUDY. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji. 2020;9(1):80-88. doi:10.18036/estubtdc.680795

Chicago

Poyraz, Nilgün, and Mehmet Mutlu. 2020. “CHARACTERIZATION OF MICROBIAL POPULATIONS OF LAKE VAN BY 16S METAGENOMICS STUDY”. Eskişehir Teknik Üniversitesi Bilim Ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji 9 (1): 80-88. https://doi.org/10.18036/estubtdc.680795.

EndNote

Poyraz N, Mutlu M (January 1, 2020) CHARACTERIZATION OF MICROBIAL POPULATIONS OF LAKE VAN BY 16S METAGENOMICS STUDY. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji 9 1 80–88.

IEEE

[1]N. Poyraz and M. Mutlu, “CHARACTERIZATION OF MICROBIAL POPULATIONS OF LAKE VAN BY 16S METAGENOMICS STUDY”, Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji, vol. 9, no. 1, pp. 80–88, Jan. 2020, doi: 10.18036/estubtdc.680795.

ISNAD

Poyraz, Nilgün - Mutlu, Mehmet. “CHARACTERIZATION OF MICROBIAL POPULATIONS OF LAKE VAN BY 16S METAGENOMICS STUDY”. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji 9/1 (January 1, 2020): 80-88. https://doi.org/10.18036/estubtdc.680795.

JAMA

1.Poyraz N, Mutlu M. CHARACTERIZATION OF MICROBIAL POPULATIONS OF LAKE VAN BY 16S METAGENOMICS STUDY. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji. 2020;9:80–88.

MLA

Poyraz, Nilgün, and Mehmet Mutlu. “CHARACTERIZATION OF MICROBIAL POPULATIONS OF LAKE VAN BY 16S METAGENOMICS STUDY”. Eskişehir Teknik Üniversitesi Bilim Ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji, vol. 9, no. 1, Jan. 2020, pp. 80-88, doi:10.18036/estubtdc.680795.

Vancouver

1.Nilgün Poyraz, Mehmet Mutlu. CHARACTERIZATION OF MICROBIAL POPULATIONS OF LAKE VAN BY 16S METAGENOMICS STUDY. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji. 2020 Jan. 1;9(1):80-8. doi:10.18036/estubtdc.680795

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