Research Article

LACCASE ISOLATION FROM SPENT MUSHROOM WASTE (AGARICUS BISPORUS): PARTIAL PURIFICATION, CHARACTERIZATION, IMMOBILIZATION AND BIOTECHNOLOGICAL APPLICATION

Volume: 10 Number: 1 January 25, 2021
Güliz Ak , Habibe Yılmaz *, Şenay Şanlıer
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LACCASE ISOLATION FROM SPENT MUSHROOM WASTE (AGARICUS BISPORUS): PARTIAL PURIFICATION, CHARACTERIZATION, IMMOBILIZATION AND BIOTECHNOLOGICAL APPLICATION

Abstract

Laccase was partially purified from mushroom compost with cheap and simple purification methods. Partially purified laccase was immobilized onto Amberlite XAD-7 resin. Immobilization conditions and textile dye (Direct Green B) decolorization of immobilized enzyme were examined. Laccase was partially purified from mushroom compost with 3.22 purification fold. Immobilization time for laccase was 30 minutes with 97% efficiency. The immobilization yield based on the activity was calculated by about 90%. The immobilization yield was calculated as nearly 95% based on the measurement of protein amount at each stage. Immobilized enzyme preserved its initial activity with an 84% rate even after its tenth use. The free enzyme lost its activity immediately when it was incubated with the dye solution under its optimal conditions (pH 3.0 and 65°C). On the contrary, the immobilized enzyme maintained its initial activity with a 53% rate when incubated with dye solution under its optimal conditions (pH 3.5 and 65°C).

Keywords

Mushroom compost, Laccase, Direct Green B, Diamine Green B, Enzyme Immobilization, Dye decolorization, Amberlite XAD-7

Supporting Institution

Mevcut değildir.

Project Number

Mevcut değildir.

Thanks

The authors acknowledge to Prof. Dr. İhsan YASA for gifting the mushroom compost. The authors have declared no conflict of interest.

References

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Details

Primary Language

English

Subjects

Ecology, Microbiology, Environmental Sciences

Journal Section

Research Article

Authors

Publication Date

January 25, 2021

Submission Date

April 24, 2020

Acceptance Date

November 30, 2020

Published in Issue

Year 2021 Volume: 10 Number: 1

DOI

https://doi.org/10.18036/estubtdc.726398

IZ

https://izlik.org/JA29AA25WL

Cite

RIS / Bibtex
APA
Ak, G., Yılmaz, H., & Şanlıer, Ş. (2021). LACCASE ISOLATION FROM SPENT MUSHROOM WASTE (AGARICUS BISPORUS): PARTIAL PURIFICATION, CHARACTERIZATION, IMMOBILIZATION AND BIOTECHNOLOGICAL APPLICATION. Eskişehir Teknik Üniversitesi Bilim Ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji, 10(1), 44-56. https://doi.org/10.18036/estubtdc.726398
AMA
1.Ak G, Yılmaz H, Şanlıer Ş. LACCASE ISOLATION FROM SPENT MUSHROOM WASTE (AGARICUS BISPORUS): PARTIAL PURIFICATION, CHARACTERIZATION, IMMOBILIZATION AND BIOTECHNOLOGICAL APPLICATION. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji. 2021;10(1):44-56. doi:10.18036/estubtdc.726398
Chicago
Ak, Güliz, Habibe Yılmaz, and Şenay Şanlıer. 2021. “LACCASE ISOLATION FROM SPENT MUSHROOM WASTE (AGARICUS BISPORUS): PARTIAL PURIFICATION, CHARACTERIZATION, IMMOBILIZATION AND BIOTECHNOLOGICAL APPLICATION”. Eskişehir Teknik Üniversitesi Bilim Ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji 10 (1): 44-56. https://doi.org/10.18036/estubtdc.726398.
EndNote
Ak G, Yılmaz H, Şanlıer Ş (January 1, 2021) LACCASE ISOLATION FROM SPENT MUSHROOM WASTE (AGARICUS BISPORUS): PARTIAL PURIFICATION, CHARACTERIZATION, IMMOBILIZATION AND BIOTECHNOLOGICAL APPLICATION. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji 10 1 44–56.
IEEE
[1]G. Ak, H. Yılmaz, and Ş. Şanlıer, “LACCASE ISOLATION FROM SPENT MUSHROOM WASTE (AGARICUS BISPORUS): PARTIAL PURIFICATION, CHARACTERIZATION, IMMOBILIZATION AND BIOTECHNOLOGICAL APPLICATION”, Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji, vol. 10, no. 1, pp. 44–56, Jan. 2021, doi: 10.18036/estubtdc.726398.
ISNAD
Ak, Güliz - Yılmaz, Habibe - Şanlıer, Şenay. “LACCASE ISOLATION FROM SPENT MUSHROOM WASTE (AGARICUS BISPORUS): PARTIAL PURIFICATION, CHARACTERIZATION, IMMOBILIZATION AND BIOTECHNOLOGICAL APPLICATION”. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji 10/1 (January 1, 2021): 44-56. https://doi.org/10.18036/estubtdc.726398.
JAMA
1.Ak G, Yılmaz H, Şanlıer Ş. LACCASE ISOLATION FROM SPENT MUSHROOM WASTE (AGARICUS BISPORUS): PARTIAL PURIFICATION, CHARACTERIZATION, IMMOBILIZATION AND BIOTECHNOLOGICAL APPLICATION. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji. 2021;10:44–56.
MLA
Ak, Güliz, et al. “LACCASE ISOLATION FROM SPENT MUSHROOM WASTE (AGARICUS BISPORUS): PARTIAL PURIFICATION, CHARACTERIZATION, IMMOBILIZATION AND BIOTECHNOLOGICAL APPLICATION”. Eskişehir Teknik Üniversitesi Bilim Ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji, vol. 10, no. 1, Jan. 2021, pp. 44-56, doi:10.18036/estubtdc.726398.
Vancouver
1.Güliz Ak, Habibe Yılmaz, Şenay Şanlıer. LACCASE ISOLATION FROM SPENT MUSHROOM WASTE (AGARICUS BISPORUS): PARTIAL PURIFICATION, CHARACTERIZATION, IMMOBILIZATION AND BIOTECHNOLOGICAL APPLICATION. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji. 2021 Jan. 1;10(1):44-56. doi:10.18036/estubtdc.726398