LACCASE ISOLATION FROM SPENT MUSHROOM WASTE (AGARICUS BISPORUS): PARTIAL PURIFICATION, CHARACTERIZATION, IMMOBILIZATION AND BIOTECHNOLOGICAL APPLICATION

Öz

Lakkaz, ucuz ve basit saflaştırma yöntemleriyle mantar kompostundan kısmen saflaştırılmıştır. Kısmen saflaştırılmış lakkaz, Amberlite XAD-7 reçinesi üzerine immobilize edilmiştir. İmmobilizasyon koşulları ve immobilize enzimin tekstil boyasının (Direct Green B) renk giderimi incelenmiştir. Lakkaz, mantar kompostundan 3,22 saflaştırma katı ile kısmen saflaştırılmıştır. Lakkaz için immobilizasyon süresi % 97 verimlilikle 30 dakikadır. Aktiviteye dayanan immobilizasyon verimi yaklaşık % 90 olarak hesaplanmıştır. İmmobilizasyon verimi, her aşamadaki protein miktarının ölçülmesine dayanılarak yaklaşık % 95 olarak hesaplanmıştır. İmmobilize enzim onuncu kullanımdan sonra bile başlangıçtaki aktivitesini % 84 oranında korumuştur. Serbest enzim, optimal koşullar (pH 3,0 ve 65°C) altında boya çözeltisi ile inkübe edildiğinde hemen aktivitesini kaybetmiştir. Aksine, immobilize enzim, optimal koşullar (pH 3,5 ve 65°C) altında boya çözeltisi ile inkübe edildiğinde başlangıç aktivitesini % 53 oranında sürdürmüştür.

Anahtar Kelimeler

• Mantar kompostu
• Lakkaz
• Direct Green B
• Diamine Green B
• Enzim immobilizasyonu
• Boya renk giderimi
• Amberlite XAD-7

LACCASE ISOLATION FROM SPENT MUSHROOM WASTE (AGARICUS BISPORUS): PARTIAL PURIFICATION, CHARACTERIZATION, IMMOBILIZATION AND BIOTECHNOLOGICAL APPLICATION

Öz

Laccase was partially purified from mushroom compost with cheap and simple purification methods. Partially purified laccase was immobilized onto Amberlite XAD-7 resin. Immobilization conditions and textile dye (Direct Green B) decolorization of immobilized enzyme were examined. Laccase was partially purified from mushroom compost with 3.22 purification fold. Immobilization time for laccase was 30 minutes with 97% efficiency. The immobilization yield based on the activity was calculated by about 90%. The immobilization yield was calculated as nearly 95% based on the measurement of protein amount at each stage. Immobilized enzyme preserved its initial activity with an 84% rate even after its tenth use. The free enzyme lost its activity immediately when it was incubated with the dye solution under its optimal conditions (pH 3.0 and 65°C). On the contrary, the immobilized enzyme maintained its initial activity with a 53% rate when incubated with dye solution under its optimal conditions (pH 3.5 and 65°C).

Anahtar Kelimeler

• Mushroom compost
• Laccase
• Direct Green B
• Diamine Green B
• Enzyme Immobilization
• Dye decolorization
• Amberlite XAD-7

Kaynakça

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