IN VITRO CYTOTOXIC ASSESSMENT OF CHITOSAN OLIGOSACCHARIDE LACTATE ON HUMAN BLOOD AND LYMPHOCYTE CELLS
Öz
Chitosan oligosaccharides (ChOSs) are the reduced products of chitosan prepared by chemical or enzymatic hydrolysis. The greater solubility and low viscosity of ChOSs are of interest. The present study was the first to evaluate the toxicity of chitosan oligosaccharide lactate (ChOSlac) in human blood. For this purpose, possible oxidative effects of ChOSlac in human whole blood (hWB) and cell viability and membrane integrity effects on lymphocytes (LYMs) were evaluated in the dose range of 10-400 μg/ml and for 24 and 48 hours treatments. Firstly, total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) were used to measure oxidative damage on hWB serum. Secondly, the cytological effects were evaluated using 2.3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H tetrazolium-5-carboxanilide inner salt (XTT) and lactate dehydrogenase (LDH) assays on LYMs. Exposure of cells to 10-200 μg/ml range doses of ChOSlac caused an increase in antioxidant activity and a decrease in oxidative stress but did not affect cytotoxicity. Conversely, the dose of 400 µg/ml caused a relative increase in oxidative stress and LDH leakage and decreased cell viability. In summary, ChOSlac has been evaluated positively at the specific dose range and exposure times in terms of human health as a contribution to its use in many areas such as being a biocompatible, biodegradable, and drug carrier molecule.
Anahtar Kelimeler
Antioxidant,, Chitosan oligosaccharide lactate,, Cytotoxic,, Human lymphocyte,, Lactate dehydrogenase,
Kaynakça
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