In Vivo Evaluation of [Gly14]-Humanin's Protective Effect Against Cisplatin-Induced Ototoxicity

Murat Yaşar; Sedat Gökmen; Fatma Atalay; İrfan Çınar; Yusuf Aydin; Musa Tatar

doi:10.18621/eurj.1803088

In Vivo Evaluation of [Gly14]-Humanin's Protective Effect Against Cisplatin-Induced Ototoxicity

Abstract

Objectives: This study aimed to evaluate in vivo the potential protective effects of [Gly14]-humanin against cisplatin-induced ototoxicity (CIO) and its modulatory effects against oxidative stress, inflammatory, and apoptotic markers

Methods: Thirty-five male Balb/c mice were randomly divided into five groups: control, cisplatin, cisplatin + [Gly14]-humanin (3 mg/kg), cisplatin + (6 mg/kg), and vitamin E. Cisplatin (10 mg/kg, b.w.) was administered as a single dose. [Gly14]-humanin was administered intraperitoneally for 14 days. Biochemical analyses (Superoxide dismutase (SOD), glutathione (GSH), and malondialdehyde (MDA)), gene expression levels (TNF-α, IL-1β, IL-6, CASP-3, CASP-9, Bcl-2/Bax), and histopathological examinations (PARP1, PARP2 immunoreactivity) were performed in cochlear tissue samples to evaluate oxidative stress, inflammation, and apoptosis.

Results: Cisplatin decreased SOD and GSH levels and increased MDA levels in the cochlear tissue (P<0.05). With the application of [Gly14]-humanin, these parameters approached their normal levels. Cisplatin increased the values of pro-inflammatory and pro-apoptotic markers (TNF-α, IL-1β, IL-6, Caspase-3 (CAS-3), Caspase-9 (CAS-9)) significantly while reducing the Bcl-2/Bax ratio (P<0.05). [Gly14]-humanin administration reversed these effects in a dose-dependent manner. Histological analysis also revealed decreased PARP1 and PARP2 immunoreactivity in [Gly14]-humanin treated groups, comparable to the positive control (vitamin E).

Conclusions: The findings of this study show that [Gly14]-humanin exhibits important protective effects against CIO by attenuating oxidative stress, inflammation, and apoptosis in cochlear tissues. Further studies evaluating the clinical efficacy and reliability of [Gly14]-humanin are now needed.

Keywords

Ethical Statement

This study was approved by the the Kastamonu University Animal Experiments Local Ethics Committee (Decision No: 2024/11-39; date: 25.10.2024). All experimental procedures involving animals were conducted in accordance with the ethical standards of the Guide for the Care and Use of Laboratory Animals published by the U.S. National Institutes of Health. All efforts were made to minimize animal suffering and to reduce the number of animals used.

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Details

Primary Language

English

Subjects

Otorhinolaryngology

Journal Section

Research Article

Authors

Murat Yaşar
0000-0003-3300-4430
Türkiye

Sedat Gökmen
0000-0002-4793-3030
Türkiye

Fatma Atalay
0000-0002-0344-1982
Türkiye

İrfan Çınar
0000-0002-9826-2556
Türkiye

Yusuf Aydin ^*
0000-0002-0964-0405
Türkiye

Musa Tatar
0000-0002-5707-8832
Türkiye

Early Pub Date

December 9, 2025

Publication Date

January 4, 2026

Submission Date

October 15, 2025

Acceptance Date

November 24, 2025

Published in Issue

Year 2026 Volume: 12 Number: 1

DOI

https://doi.org/10.18621/eurj.1803088

IZ

https://izlik.org/JA59RC75UT

Cite

RIS / Bibtex

AMA

1.Yaşar M, Gökmen S, Atalay F, Çınar İ, Aydin Y, Tatar M. In Vivo Evaluation of [Gly14]-Humanin’s Protective Effect Against Cisplatin-Induced Ototoxicity. Eur Res J. 2026;12(1):119-128. doi:10.18621/eurj.1803088