Association Between Cardiopulmonary Bypass–Induced Sirtuin-1 Suppression and Apoptosis

Bişar Amaç; Ömer Göç; Mesut Engin; Senol Yavuz

doi:10.18621/eurj.1861383

Association Between Cardiopulmonary Bypass–Induced Sirtuin-1 Suppression and Apoptosis

Abstract

Objectives: The aim of this study is to investigate, at the molecular level, the effect of cardiopulmonary bypass (CPB) on Sirtuin-1 levels, an epigenetic-metabolic regulatory protein, and the relationship between this change and Caspase-3, one of the key executor molecules of cellular apoptosis.

Methods: Patients undergoing open heart surgery under CPB were included in the study. Sirtuin-1 and Caspase-3 levels were measured in venous blood samples taken from patients during the preoperative, intraoperative, and postoperative periods. The Wilcoxon Signed Ranks test was used to analyze differences between time points.

Results: Sirtuin-1 levels decreased significantly during the intraoperative period compared to preoperative values (Z= -6.212, P<0.001). Similarly, postoperative Sirtuin-1 levels were significantly lower compared to both the preoperative (Z= -6.229, P<0.001) and intraoperative periods (Z= -6.186, P<0.001). Caspase-3 levels showed a significant increase in the intraoperative period compared to the preoperative period (Z= −6.262, P<0.001). Postoperative Caspase-3 levels were significantly higher than both the preoperative (Z= -6.196, P<0.001) and intraoperative periods (Z= -6.203, P<0.001).

Conclusions: These findings indicate that CPB suppresses circulating Sirtuin-1 levels and that this suppression is associated with increased apoptosis. CPB-induced Sirtuin-1 inhibition may be one of the molecular mechanisms of surgical-related cellular damage. Sirtuin-1 may be considered a potential molecular target for preventing CPB-related myocardial and systemic damage.

Keywords

Ethical Statement

This study was approved by the Harran University Clinical Research Ethics Committee (Decision No: HRÜ/25.18.33; date: 17.11.2025. All procedures were conducted in accordance with the ethical standards of the institutional and national research committee and with the 1964 Helsinki Declaration and its later amendments. Furthermore, informed consent was obtained from all participants involved in the study.

References

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Details

Primary Language

English

Subjects

Cardiovascular Surgery

Journal Section

Research Article

Authors

Bişar Amaç ^*
0000-0003-0320-4239
Türkiye

Ömer Göç
0000-0002-3047-6232
Türkiye

Mesut Engin
0000-0003-2418-5823
Türkiye

Senol Yavuz
0000-0001-5246-0808
Türkiye

Early Pub Date

February 11, 2026

Publication Date

March 1, 2026

Submission Date

January 11, 2026

Acceptance Date

February 11, 2026

Published in Issue

Year 2026 Volume: 12 Number: 3

DOI

https://doi.org/10.18621/eurj.1861383

IZ

https://izlik.org/JA26MH76GN

Cite

RIS / Bibtex

AMA

1.Amaç B, Göç Ö, Engin M, Yavuz S. Association Between Cardiopulmonary Bypass–Induced Sirtuin-1 Suppression and Apoptosis. Eur Res J. 2026;12(3):339-346. doi:10.18621/eurj.1861383