Yıl 2019, Cilt 5 , Sayı 6, Sayfalar 996 - 1000 2019-11-04

Study of biofilm formation in Salmonella species isolated from food

Mohammad Mehdi Soltan Dallal [1] , Mohammad Khalifeh-Gholi [2] , Hojjat Rahmani [3] , Sara Sharifi-yazdi [4] , Shabnam Haghighat Khajavi [5] , Mohammad Kazem Sharifi Yazdi [6]


Objectives: Biofilms are defined as communities of organisms attached to a surface and producing an extracellular matrix, in which the bacteria are imbedded. Infections with Salmonella species represent a major health problem and a significant burden on food industry. Biofilm formation is one of the causes of pathogenicity of Salmonella species, especially in the food industry, which allows bacteria to bind to different levels. Many outbreaks have been associated with biofilms, because they quickly resist anti-microbial and cleansing agents. The aim of this research was to study the capability of biofilm formation by Salmonella species isolated from food.

Methods: A total of 8 Salmonella species were isolated from 400 samples of red meat, chicken, eggs, and vegetables. Identification was carried out by conventional biochemical tests and serotyping. The capability of biofilm production was measured by titration in Crystal Violet microplate.

Results: In the phenotypic study of Salmonella isolates with turbidity method at 550 nm without acetic acid, only 2 (25%) of isolates were able to produce biofilm. both of isolates belonged to the group D of Salmonella.

Conclusions: The capability of the isolates to form biofilm reveals the potential ability to resist antimicrobial chemotherapy, therefore higher levels of hygiene in production, packaging, and supply are necessary.

Salmonella, biofilms, foodborne disease
  • [1] Austin JW, Sanders G, Kay W, Collinson SK. Thin aggregative fimbriae enhance Salmonella enteritidis biofilm formation. FEMS Microbiol Lett 1998;162:295-301.
  • [2] Giaouris ED, Nychas GJ. The adherence of Salmonella enteritidis PT4 to stainless steel: The importance of the air-liquid interface and nutrient availability. J Food Microbiol 2006;23:747-52.
  • [3] Duran N, Ozer B, Duran GG, Onlen Y, Demir C. Antibiotic resistance genes & susceptibility patterns in staphylococci. Indian J Med Res 2012;135:389-96.
  • [4] Atshan SS, Nor Shamsudin M, Sekawi Z, Lung LT, Hamat RA, Karunanidhi A, et al. Prevalence of adhesion and regulation of biofilm-related genes in different clones of Staphylococcus aureus. J Biomed Biotechnol 2012;2012:976972.
  • [5] Van Houdt R, Michiels CW. Biofilm formation and the food industry, a focus on the bacterial outer surface. J Appl Microbiol 2010;109:1117-31.
  • [6] Palmer J, Flint S, Brooks J. Bacterial cell attachment, the beginning of a biofilm. J Ind Microbiol Biotechnol 2007;34:577-88.
  • [7] Bryers JD. Biofilms II: Process analysis and application. New York: John Wiley and Sons, 2000.
  • [8] Bae YM, Baek SY, Lee SY. Resistance of pathogenic bacteria on the surface of stainless steel depending on attachment form and efficacy of chemical sanitizers. Int J Food Microbiol 2012;153:465-73.
  • [9] Wu Y, Park KC, Choi BG, Park JH, Yoon KS. The Antibiofilm effect of Ginkgo biloba extract against Salmonella and Listeria isolates from Poultry. Foodborne Pathog Dis 2016;13:229-38.
  • [10] Chen D, Zhao T, Doyle MP. Control of pathogens in biofilms on the surface of stainless steel by levulinic acid plus sodium dodecyl sulfate. Int J Food Microbiol 2015;207:1-7.
  • [11] Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing; 25th informational supplement. M100-S25. Clinical and Laboratory Standards Institute, Wayne, PA, 2015;35(3):1-231.
  • [12] Peeters E, Nelis HJ, Coenye T. Comparison of multiple methods for quantification of microbial biofilms grown in microtiter plates. J Microbiol Methods 2008;72:157-65.
  • [13] Hood SK, Zottola EA. Adherence tostainless steel foodborne microorganisms during growth in model food systems. Int J Food Microbiol 1997;37:145-53.
  • [14] Joseph B, Otta SK, Karunasagar I, Karunasagar I. Biofilm formation by Salmonella spp. on food contact surfaces and their sensitivity to sanitizers. Int J Food Microbiol 2001;64:367-72.
  • [15] Sivapalasingam S, Friedman CR, Cohen L, Tauxe RV. Fresh produce: a growing cause of outbreaks of foodborne illness in the United States, 1973 through 1997. J Food Prot 2004;67:2342-53.
  • [16] Igbinosa IH. Biofilm formation of Salmonella species isolated from fresh cabbage and spinach. J Appl Sci Environ Manag 2015;19:45-50.
  • [17] Wang H, Ding S, Wang G, Xu X, Zhou G. In situ characterization and analysis of Salmonella biofilm formation under meat processing environments using a combined microscopic and spectroscopic approach. Int J Food Microbiol 2013;167:293-302.
  • [18] Learn-Han L, Yoke-Kqueen C, Shiran MS, Sabrina S, Noor Zaleha AS, Sim JH, et al. Molecular characterization and antimicrobial resistance profiling of Salmonella enterica subsp. enterica isolated from ‘Selom’ (Oenanthe stolonifera). Int Food Res J 2009;16:191-202.
  • [19] Lertworapreecha M, Sutthimusik S, Tontikapong K. Antimicrobial resistance in Salmonella enterica isolated from pork, chicken, and vegetables in southern Thailand. Jundishapur J Microbiol 2013;6:36-41.
  • [20] Kozak GK, MacDonald D, Landry L, Farber JM. Foodborne outbreaks in Canada linked to produce: 2001 through 2009. J Food Prot 2003;76:173-83.
  • [21] Shah DH, Zhou X, Addwebi T, Davis MA, Orfe L, Call DR, et al. Cell invasion of poultry-associated Samonella enterica serovar Enteritidis isolates is associated with pathogenicity, motility and proteins secreted by the type III secretion system. Microbiology 211;157(Pt 5):1428-35.
Birincil Dil en
Konular Enfeksiyon Hastalıkları, Biyokimya ve Moleküler Biyoloji, Mikrobiyoloji, Sağlık Bilimleri ve Hizmetleri, Temel Sağlık Hizmetleri
Bölüm Original Article
Yazarlar

Orcid: 0000-0002-3421-3974
Yazar: Mohammad Mehdi Soltan Dallal (Sorumlu Yazar)
Kurum: Department of Pathobiology, Division of Microbiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Ülke: Iran


Orcid: 0000-0003-0474-2477
Yazar: Mohammad Khalifeh-Gholi
Kurum: Department of Microbiology and Immunology, Cellular and Molecular, Faculty of Medicine, Qom University of Medical Sciences, Qom, Iran
Ülke: Iran


Orcid: 0000-0003-3176-1614
Yazar: Hojjat Rahmani
Kurum: Department of Management Sciences and Health Economics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Ülke: Iran


Orcid: 0000-0002-1974-4021
Yazar: Sara Sharifi-yazdi
Kurum: School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
Ülke: Iran


Orcid: 0000-0001-5146-5121
Yazar: Shabnam Haghighat Khajavi
Kurum: Department of Food Sciences and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran
Ülke: Iran


Orcid: 0000-0001-9060-5908
Yazar: Mohammad Kazem Sharifi Yazdi
Kurum: Department of Medical Laboratory Sciences, Zoonosis Research Center, Tehran University of Medical Sciences, Tehran, Iran
Ülke: Iran


Destekleyen Kurum Tehran University of Medical Sciences (TUMS)
Proje Numarası 32414
Tarihler

Yayımlanma Tarihi : 4 Kasım 2019

EndNote %0 The European Research Journal Study of biofilm formation in Salmonella species isolated from food %A Mohammad Mehdi Soltan Dallal , Mohammad Khalifeh-Gholi , Hojjat Rahmani , Sara Sharifi-yazdi , Shabnam Haghighat Khajavi , Mohammad Kazem Sharifi Yazdi %T Study of biofilm formation in Salmonella species isolated from food %D 2019 %J The European Research Journal %P -2149-3189 %V 5 %N 6 %R doi: 10.18621/eurj.434298 %U 10.18621/eurj.434298