Cytotoxic and Apoptotic Effects of Carmofur and Vitamin C Combination on HCT116 Colon Cancer Cells

Abstract

Objective: In recent years, especially combined treatment options that will reduce the side effects of drugs have attracted attention. Carmofur is a potent new agent being investigated in the treatment of cancer. This study was aimed to investigate the cytotoxic and apoptotic effects of carmofur+vitamin C combination in cancer cells. Materials and Methods: In this study, carmofur in the range of 7.8-250 µM, Vitamin C in 7.8µM-2mM and Carmofur+Vitamin C in different concentrations were evaluated on HCT116 cells by MTT test. In addition, the cell death pathway was determined by apoptosis-necrosis assay. Results: The IC50 value of carmofur in HCT116 cells was 8 µM, vitamin C was not effective at low doses, and the IC50 value was 2.2 mM. When carmofur+Vitamin C were applied to HCT116 cells, 4 µM carmofur and 125 µM vitamin C together inhibited half of the cells (IC50), while 4 µM carmofur and 2 mM vitamin C inhibited half of the cells similarly. Conclusion: Vitamin C doubled the anticancer effect of carmofur. As a result, it was observed that vitamin C supplementation significantly increased cell death in anticancer drug administration. In addition, it was determined that the addition of vitamin C significantly increased the apoptotic activity.

Keywords

• Carmofur
• Vitamin C
• combined therapy
• colon cancer
• HCT116

HCT116 KOLON KANSERİ HÜCRELERİNDE KARMOFUR VE C VİTAMİNİ KOMBİNASYONUNUN SİTOTOKSİK VE APOPTOTİK ETKİLERİ

Abstract

Amaç: Kanser tedavisinde birçok yaklaşım bulunmasına rağmen, daha etkili tedavilerin geliştirilmesine ihtiyaç duyulmaktadır. Son yıllarda özellikle ilaçların yan etkisini azaltacak kombine tedavi seçenekleri ilgi çekmektedir. Seramidaz inhibitörü olan karmofur, kanser tedavisinde araştırılan yeni güçlü bir adaydır. Bu çalışmada, özellikle C vitamini gibi doğal bileşiklerin karmofurla birlikte kullanılmasının kanser hücrelerinde sitotoksite ve apoptotik aktiviteyi nasıl etkilediğinin araştırılması amaçlanmıştır. Gereç ve Yöntem: Bu çalışmada, HCT116 kolon kanseri üzerinde, karmofur 7,8-250 µM aralığında, C Vitamini 7.8µM-2mM aralığında ve Karmofur+C Vitamini ise, farklı konsantrasyonlarda MTT testi ile, değerlendirildi. Ayrıca hücre ölümü yolu apoptoz-nekroz tayini ile belirlendi. Bulgular: Karmofurun kolon kanseri hücrelerinde IC50 değerinin 8 µM olduğu, C vitaminin düşük dozlarda etkili olmadığı, IC50 değerinin ise 2.2 mM olduğu tespit edildi. HCT116 hücrelerine karmofur ve C Vitamini birlikte uygulandığında, 4 µM karmofur ile 125 µM C vitamini birlikte hücrelerin yarısını inhibe ederken (IC50), 4 µM karmofur ile 2 mM C vitaminin de benzer şekilde hücrelerin yarısını inhibe ettiği görüldü. Sonuç: C vitamini antikanser ilaçlarla birlikte antikanser ilacın etkisini 2 katına çıkardı. Sonuç olarak antikanser ilaç uygulamasında, C vitamini desteğinin hücre ölümünü anlamlı bir şekilde arttırdığı görüldü. Ayrıca, C vitamini ilavesinin apoptotik aktiviteyi de önemli bir şekilde arttırdığı tespit edildi.

Keywords

• Karmofur
• C Vitamini
• Kombine tedavi
• Kolon kanseri
• HCT116

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