Association of XRCC3 Thr241Met Polymorphism with Renal Cell Carcinoma in a Turkish Population

Abstract

Objective: Renal cell carcinoma (RCC) is considered as a major type of cancer of the kidney and is estimated to account for about 2-3% of all malignancies in adults. DNA repair mechanisms play a crucial role in defending genomic integrity against DNA damage, and defects in DNA repair mechanisms are associated with cancer susceptibility. The XRCC3 gene plays a pivotal role in the DNA repair system through homologous recombination and chromosomal activity. Therefore, our research aimed to clarify whether XRCC3 Thr241Met polymorphism affects the initiation and progression of RCC. Materials and Methods: This study included 129 patients with RCC and 212 healthy individuals. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was employed to analyze XRCC3 Thr241Met gene polymorphism using SPSS 23 software to facilitate data analysis. Results: Our results revealed no remarkable differences in the genotype and allele frequencies of the Thr241Met polymorphism of XRCC3 between the patient and control groups. However, a greater risk of RCC was reported for the variant (Met/Met) genotype of XRCC3 gene polymorphism, particularly among smokers. Conclusion: Although the XRCC3 Thr241Met polymorphism may not significantly influence RCC initiation and progression in a Turkish population, smoking seems to amplify the risk associated with the (Met/Met) genotype of XRCC3 gene polymorphism.

Keywords

• Renal cell carcinoma
• XRCC3
• polymorphism
• Thr241Met
• PCR-RFLP

References

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