Zebra Balığı Embriyolarında Nörotoksin Maruziyetine Karşı Oluşan Yanıta Düşük Doz X-ışınının Etkileri

Year 2025, Volume: 15 Issue: 3, 261 - 268, 31.12.2025

Ezgi Cahide Aydaş Bayramov , Merih Beler , Gizem Eğilmezer , Zülal Mızrak , Semanur Işikoğlu , Selma Yaltkaya , İsmail Ünal , Derya Cansız , Ebru Emekli Alturfan , Şebnem Erçalık Yalçınkaya

https://doi.org/10.26650/experimed.1796919

Abstract

Amaç: Diş hekimliğinde tanı amaçlı görüntüleme yöntemleri sıklıkla kullanılmaktadır. Dijital görüntülemede kullanılan X-ışını dozları düşük olmakla birlikte, tekrarlanan maruziyetler sağlık açısından risk oluşturmaktadır. 1-metil-4-fenil-1,2,3,6-tetrahidropiridin (MPTP) nörotoksini, oksidatif stres aracılığıyla dopaminerjik nöron kaybına neden olur. Bu çalışmanın hipotezi düşük doz X-ışınının (DDXI), MPTP maruziyetindeki zebra balığı embriyolarında oksidan–antioksidan yanıtta değişikliğe neden olabileceğidir.
Gereç ve Yöntemler: Zebra balığı embriyoları kontrol, MPTP, DDXI ve DDXI+MPTP gruplarına ayrılmıştır. Maruziyet gruplarında dental X-ışını cihazı (0,08 saniye) ve 400 µM MPTP kullanılmıştır. Gelişimsel parametreler fertilizasyon sonrası 72. saate (hpf) kadar izlenmiştir. 72 hpf’de lipid peroksidasyonu (LPO), nitrik oksit (NO) seviyeleriyle, süperoksit dismutaz (SOD) ve glutatyon S-transferaz (GST) aktiviteleri ölçülmüştür.
Bulgular: SOD, GST aktiviteleri ile LPO ve NO seviyeleri tüm maruziyet gruplarında anlamlı şekilde artmıştır. Özellikle DDXI +MPTP maruziyetinde, yalnızca MPTP maruziyetine kıyasla LPO seviyeleri azalmış; SOD ve GST aktiviteleri ise tek başına DDXI ve MPTP gruplarına göre anlamlı derecede yüksek izlenmiştir. Ayrıca DDXI maruziyetinde perikardiyal ödem ve skolyoz gibi gelişimsel anomaliler gözlenmiştir.
Sonuç: DDXI+MPTP maruziyeti zebra balığı embriyolarında oksidan–antioksidan dengede bozukluğa neden olmuştur. DDXI’nın etkileri çok yönlüdür ve MPTP gibi diğer stres faktörleri ile etkileşimini daha iyi değerlendirmek için ek çalışmalara ihtiyaç vardır.

Keywords

Zebra balığı , Radyasyon , MPTP , Oksidatif stres.

Low-dose X-ray Radiation Alters the Response to Neurotoxin Exposure in Zebrafish Embryos

Abstract

Objective: Dental diagnostic imaging is widely used in dentistry. Although X-ray radiation doses in modern digital dental imaging are low, repeated exposures increase the health risks. The neurotoxin 1-methyl-4 phenyl-1,2,3,6-tetrahydropyridine (MPTP) specifically impairs dopaminergic neurons by inducing oxidative stress. We hypothesized that low-dose X-ray radiation (LDXR) exposure may modulate oxidant–antioxidant responses in MPTP-exposed embryos.

Materials and Methods: Zebrafish embryos were categorized as the control, MPTP, LDXR, and LDXR+MPTP groups. Treatments were performed using an X-ray device for dental purposes and 400 µM MPTP. Devel opmental parameters were monitored until 72 h post fertilization (hpf). Biochemical parameters related to the oxidant and antioxidant status were investigated at 72 hpf.

Results: Superoxide dismutase (SOD), glutathione-S transferase (GST) activities, lipid peroxidation (LPO), and nitric oxide (NO) levels were significantly elevated in all exposure groups. Notably, LDXR+MPTP exposure reduced LPO levels compared with MPTP exposure, while SOD and GST were significantly higher than those in the LDXR and MPTP exposed groups. In addition, LDXR exposure induced developmental abnormalities, including pericardial edema and scoliosis.

Conclusion: LDXR+MPTP exposure interrupted the oxidant-antioxidant equilibrium in zebrafish embryos. Overall, the effects of LDXR are multifaceted, and additional studies are needed to better comprehend its interactions with other stressors such as MPTP

Keywords

Zebrafish , Radiation , MPTP , Oxidative stress.

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