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Histone Deacetylase, Xanthine Oxidase and Urease Inhibitory Activities of Eremurus spectabilisM. Bieb. Extracts

Year 2021, , 156 - 161, 08.12.2021
https://doi.org/10.26650/experimed.2021.913680

Abstract

Objective: Natural ingredients derived from plants (especially ed-ible ones) continue to be investigated for understanding whether they have therapeutic/preventive effects on certain ailments, such as cancer and gastrointestinal disorders. The main goal of the current work is to investigate the inhibitory potential of Eremurus (E) spectabilis M. Bieb. extracts on histone deacetylase (HDAC), xanthine oxidase (XO) and urease activities.

Material and Method: Fresh E. spectabilis leaves were obtained from a local market in Eyup/Istanbul. Inhibitory activities of ethanolic and ethyl acetate extracts on HDAC, XO and urease were examined.

Results: According to our findings, ethanolic and ethyl acetate extracts of E. spectabilis both have revealed apparent inhibitory effects against all examined enzymes. Ethanolic extract showed a better inhibitory effect on HDAC than ethyl acetate extract. On the other hand, the inhibitory effects of ethyl acetate extract against both XO and urease were higher than the ethanolic extract.

Conclusion: Consequently, due to the inhibitory effects of the plant being studied, it might be suggested that it can be used for medicinal purposes, in raw extract form, or as a source of bioactive compounds.

References

  • 1. Yuan H, Ma Q, Ye L, Piao G. The traditional medicine and modern medicine from natural products. Molecules 2016; 21(5): 559. [CrossRef] google scholar
  • 2. The genus Eremurus. (2021, May 03). Available from: http://www. theplantlist.org/tpl1.1/search?q=Eremurus google scholar
  • 3. Salehi B, Ayatollahi SA, Segura-Carretero A, Kobarfard F, Contreras MDM, Faizi M, et al. Bioactive chemical compounds in Eremurus persicus (Joub. & Spach) Boiss. essential oil and their health impli-cations. Cell Mol Biol 2017; 63(9): 1-7. [CrossRef] google scholar
  • 4. Cinar A, Ay ST, Ayas, F, Karabak S, Guzelsoy N, Ucurum O. Foxtail lilly (Eremurus spectabilis M. Bieb.) as priority species biodiversity for food and nutrition project of Turkey. Anadolu J Aegean Agric Res Inst 2017; 27(2): 69-73. google scholar
  • 5. Aysu T, Demirbaş A, Bengü AŞ, Küçük MM. Evaluation of Eremurus spectabilis for production of bio-oils with supercritical solvents. Process Saf Environ Prot 2015; 94: 339-49. [CrossRef] google scholar
  • 6. Tosun M, Ercisli S, Ozer H, Turan M, Polat T, Ozturk E, et al. Chem-ical composition and antioxidant activity of foxtail lily (Eremurus spectabilis). Acta Sci Pol Hortorum Cultus 2012; 11(3): 145-53. google scholar
  • 7. Gaggeri R, Rossi D, Mahmood K, Gozzini D, Mannucci B, Corana F, et al. Towards elucidating Eremurus root remedy: Chemical profil-ing and preliminary biological investigations of Eremurus persi-cus and Eremurus spectabilis root ethanolic extracts. J Med Plant Res 2015; 8(41): 1038-48. [CrossRef] google scholar
  • 8. Gaggeri R, Rossi D, Azzolina O, Ahmed KM, Avanzini A, Mantelli M, et al. Anti-inflammatory properties of ethanolic root extract of Er-emurus persicus (Jaub and Spach) Boiss, a Kurdish herbal remedy. J Med Plant Res 2013; 7(23): 1730-5. google scholar
  • 9. Dashti M, Tavakoli H, Zarif Ketabi H, Paryab A. Ecological require-ments of plant of Eremurus (Eremurus spectabilis MB) in Khorasan province. Iran J Range Desert Res 2005; 12(2): 153-65. google scholar
  • 10. Prakash V, Sagar A. Analysis of Achyranthes aspera L. extracts for their a-amylase and urease inhibitory activities. J Drug Deliv Ther 2021; 11(2): 108-12. [CrossRef] google scholar
  • 11. Phaniendra A, Jestadi DB, Periyasamy L. Free radicals: Properties, sources, targets, and their implication in various diseases. Indian J Clin Biochem 2015; 30(1): 11-26. [CrossRef] google scholar
  • 12. Ganai SA. Plant-derived flavone apigenin: The small-molecule with promising activity against therapeutically resistant prostate cancer. Biomed Pharmacother 2017; 85: 47-56. [CrossRef] google scholar
  • 13. Ganai SA, Sheikh FA, Baba ZA. Plant flavone Chrysin as an emerg-ing histone deacetylase inhibitor for prosperous epigenetic-based anticancer therapy. Phytother Res 2021; 35(2): 823-34. [CrossRef] google scholar
  • 14. Bortolotti M, Polito L, Battelli MG, Bolognesi A. Xanthine oxidore-ductase: One enzyme for multiple physiological tasks. Redox Biol 2021; 41: 101882. [CrossRef] google scholar
  • 15. Battelli MG, Bortolotti M, Polito L, Bolognesi A. Metabolic syn-drome and cancer risk: The role of xanthine oxidoreductase. Re-dox Biol 2019; 21: 101070. [CrossRef] google scholar
  • 16. Khan MA, Aslam M, Shahzad SA, Khan ZA, Khan NA, Ali M, et al. Design and synthesis of thiobarbituric acid analogues as potent urease inhibitors. J Mol Struct 2021; 1231: 129959. [CrossRef] google scholar
  • 17. Phull AR, Ali A, Rafiq M, Tahir T, Majid A, Seo SY, et al. Antioxidant potential, urease and acetylcholine esterase inhibitory activity and phytochemical analysis of selected medicinal plants from the Republic of Korea. Explor Res Hypothesis Med 2021; 6(2): 51-59. [CrossRef] google scholar
  • 18. Abdullahi A, Hamzah RU, Jigam AA, Yahya A, Kabiru AY, Muham-mad H, et al. Inhibitory activity of xanthine oxidase by fractions Crateva adansonii. J Acute Dis 2012; 1(2): 126-9. [CrossRef] google scholar
  • 19. Van Slyke DD, Archibald RM. Manometrie, titrimetric and colori-metric methods for measurements of urease activity. J Biol Chem 1944; 154: 623-42. [CrossRef] google scholar
  • 20. Ganai SA, Sheikh FA, Baba ZA, Mir MA, Mantoo MA, Yatoo MA. Anticancer activity of the plant flavonoid luteolin against preclin-ical models of various cancers and insights on different signalling mechanisms modulated. Phytother Res 2021 Feb 13. [Epub ahead of print]. [CrossRef] google scholar
  • 21. Karaman K, Polat B, Ozturk I, Sagdic O, Ozdemir C. Volatile compounds and bioactivity of Eremurus spectabilis (Ciris), a Turkish wild edible vegetable. J Med Food 2011; 14(10): 1238-43. [CrossRef] google scholar
  • 22. Ghasemi S, Xu S, Nabavi SM, Amirkhani MA, Sureda A, Tejada S, et al. Epigenetic targeting of cancer stem cells by polyphenols (can-cer stem cells targeting). Phytother Res 2021 Feb 22. [Epub ahead of print]. [CrossRef] google scholar
  • 23. Karaoglan ES, Gundogdu G, Secme M, Onur S, Miloglu FD, Dodur-ga Y, et al. Eremurus spectabilis, rich source of isoorientin: Isola-tion, quantification and anti-cancer activity on SH-SY5Y neuro-blastoma cells. Curr Pharm Anal 2018; 14(6): 578-85. [CrossRef] google scholar
  • 24. Abubaker SR, Hidayat HJ. Anti tumor potential of local aslerk (Er-emurus spectabilis) leaf extracts by HPLC and applying on cancer cell lines in vitro. Iraqi J Cancer Med Genet 2015; 8(2): 123-8. google scholar
  • 25. Burmaoglu S, Ozcan S, Balcioglu S, Gencel M, Noma SAA, Essiz S, et al. Synthesis, biological evaluation and molecular docking studies of bis-chalcone derivatives as xanthine oxidase inhibitors and an-ticancer agents. Bioorg Chem 2019; 91: 103149. [CrossRef] google scholar
  • 26. Xu H, Li C, Mozziconacci O, Zhu R, Xu Y, Tang Y, et al. Xanthine oxidase-mediated oxidative stress promotes cancer cell-specific apoptosis. Free Radic Biol Med 2019; 139: 70-9. [CrossRef] google scholar
  • 27. Bircan B, Kırbağ S. Determination of antioxidant and antimicrobial properties of Eremurus spectabilis Bieb. Artvin Çoruh Üniv Orman Fak Derg 2015; 16(2): 176-86. [CrossRef] google scholar
  • 28. Symonowicz M, Kolanek M. Flavonoids and their properties to form chelate complexes. Biotechnol Food Sci 2012; 76(1): 35-41. google scholar
  • 29. El Aanachi S, Gali L, Rammali S, Bensouici C, Aassila H, Dari K. In vitro study of the antioxidant, photoprotective, anti-tyrosinase, and anti-urease effects of methanolic extracts from leaves of six Moroccan Lamiaceae. J Food Meas Charact 2021; 15(2): 1785-95. [CrossRef] google scholar
  • 30. Karakaya L, Akgül Y, Nalbantsoy A. Chemical constituents and in vitro biological activities of Eremurus spectabilis leaves. Nat Prod Res 2017; 31(15): 1786-91. [CrossRef] google scholar
  • 31. Mushtaq A, Hussein Masoodi M, Farooq Wali A, Ganai A. Total phe-nolic content, total flavonoid content, in vitro antioxidant activity and antimicrobial activity against human pathogenic bacteria of Eremurus himalaicus-An edible herb of North Western Himalayas. Free Radic Antiox 2017; 7(1): 90-4. [CrossRef] google scholar
  • 32. Nile S, Keum YS, Nile AS, Jalde SS, Patel RV. Antioxidant, anti-in-flammatory, and enzyme inhibitory activity of natural plant fla-vonoids and their synthesized derivatives. J Biochem Mol Toxicol 2017; 32(1): e22002. [CrossRef] google scholar

Eremurus spectabilis Bieb. Ekstrelerinin Histon Deasetilaz, Ksantin Oksidaz ve Üreaz İnhibitör Aktiviteleri

Year 2021, , 156 - 161, 08.12.2021
https://doi.org/10.26650/experimed.2021.913680

Abstract

Amaç: Bitkilerden elde edilen doğal bileşiklerin (özellikle yenilebilir olanlar), kanser ve gastrointestinal bozukluklar gibi belirli rahatsız-lıklar üzerinde tedavi edici/önleyici etkilerinin olup olmadığı araştırılmaya devam etmektedir. Çalışmanın temel amacı, Eremurus (E) spectabilis M. Bieb. ekstrelerinin histon deasetilaz (HDAC), ksantin oksidaz (XO) ve üreaz aktiviteleri üzerindeki inhibitör etkilerini araştırmaktır.

Gereç ve Yöntem: E. spectabilis’in taze yaprakları, Eyüp/İstanbul’daki, yerel bir pazardan temin edilmiştir. Etanol ve etil asetat ekstrelerinin HDAC, XO ve üreaz üzerindeki inhibitör aktiviteleri incelenmiştir.

Bulgular: Elde edilen sonuçlara göre, E. spectabilis'in hem etanol hem de etil asetat ekstrelerinin incelenen tüm enzimler üzerinde belirgin inhibitör etkiye sahip olduğu bulunmuştur. Etanol ekstresi, HDAC üzerinde etil asetat ekstresinden daha iyi bir inhibisyon etkisi göstermiştir. Etil asetat ekstresinin ise, hem XO hem de üreaza karşı inhibisyon etkisinin, etanol ekstresinden daha yüksek olduğu görülmüştür.

Sonuç: Sonuç olarak, incelenen bitkinin gösterdiği inhibitör etkiler nedeni ile, bu bitkinin tıbbi amaçla, ham ekstre şeklinde veya biyoaktif bileşik kaynağı olarak kullanılabileceği ileri sürülebilir.

References

  • 1. Yuan H, Ma Q, Ye L, Piao G. The traditional medicine and modern medicine from natural products. Molecules 2016; 21(5): 559. [CrossRef] google scholar
  • 2. The genus Eremurus. (2021, May 03). Available from: http://www. theplantlist.org/tpl1.1/search?q=Eremurus google scholar
  • 3. Salehi B, Ayatollahi SA, Segura-Carretero A, Kobarfard F, Contreras MDM, Faizi M, et al. Bioactive chemical compounds in Eremurus persicus (Joub. & Spach) Boiss. essential oil and their health impli-cations. Cell Mol Biol 2017; 63(9): 1-7. [CrossRef] google scholar
  • 4. Cinar A, Ay ST, Ayas, F, Karabak S, Guzelsoy N, Ucurum O. Foxtail lilly (Eremurus spectabilis M. Bieb.) as priority species biodiversity for food and nutrition project of Turkey. Anadolu J Aegean Agric Res Inst 2017; 27(2): 69-73. google scholar
  • 5. Aysu T, Demirbaş A, Bengü AŞ, Küçük MM. Evaluation of Eremurus spectabilis for production of bio-oils with supercritical solvents. Process Saf Environ Prot 2015; 94: 339-49. [CrossRef] google scholar
  • 6. Tosun M, Ercisli S, Ozer H, Turan M, Polat T, Ozturk E, et al. Chem-ical composition and antioxidant activity of foxtail lily (Eremurus spectabilis). Acta Sci Pol Hortorum Cultus 2012; 11(3): 145-53. google scholar
  • 7. Gaggeri R, Rossi D, Mahmood K, Gozzini D, Mannucci B, Corana F, et al. Towards elucidating Eremurus root remedy: Chemical profil-ing and preliminary biological investigations of Eremurus persi-cus and Eremurus spectabilis root ethanolic extracts. J Med Plant Res 2015; 8(41): 1038-48. [CrossRef] google scholar
  • 8. Gaggeri R, Rossi D, Azzolina O, Ahmed KM, Avanzini A, Mantelli M, et al. Anti-inflammatory properties of ethanolic root extract of Er-emurus persicus (Jaub and Spach) Boiss, a Kurdish herbal remedy. J Med Plant Res 2013; 7(23): 1730-5. google scholar
  • 9. Dashti M, Tavakoli H, Zarif Ketabi H, Paryab A. Ecological require-ments of plant of Eremurus (Eremurus spectabilis MB) in Khorasan province. Iran J Range Desert Res 2005; 12(2): 153-65. google scholar
  • 10. Prakash V, Sagar A. Analysis of Achyranthes aspera L. extracts for their a-amylase and urease inhibitory activities. J Drug Deliv Ther 2021; 11(2): 108-12. [CrossRef] google scholar
  • 11. Phaniendra A, Jestadi DB, Periyasamy L. Free radicals: Properties, sources, targets, and their implication in various diseases. Indian J Clin Biochem 2015; 30(1): 11-26. [CrossRef] google scholar
  • 12. Ganai SA. Plant-derived flavone apigenin: The small-molecule with promising activity against therapeutically resistant prostate cancer. Biomed Pharmacother 2017; 85: 47-56. [CrossRef] google scholar
  • 13. Ganai SA, Sheikh FA, Baba ZA. Plant flavone Chrysin as an emerg-ing histone deacetylase inhibitor for prosperous epigenetic-based anticancer therapy. Phytother Res 2021; 35(2): 823-34. [CrossRef] google scholar
  • 14. Bortolotti M, Polito L, Battelli MG, Bolognesi A. Xanthine oxidore-ductase: One enzyme for multiple physiological tasks. Redox Biol 2021; 41: 101882. [CrossRef] google scholar
  • 15. Battelli MG, Bortolotti M, Polito L, Bolognesi A. Metabolic syn-drome and cancer risk: The role of xanthine oxidoreductase. Re-dox Biol 2019; 21: 101070. [CrossRef] google scholar
  • 16. Khan MA, Aslam M, Shahzad SA, Khan ZA, Khan NA, Ali M, et al. Design and synthesis of thiobarbituric acid analogues as potent urease inhibitors. J Mol Struct 2021; 1231: 129959. [CrossRef] google scholar
  • 17. Phull AR, Ali A, Rafiq M, Tahir T, Majid A, Seo SY, et al. Antioxidant potential, urease and acetylcholine esterase inhibitory activity and phytochemical analysis of selected medicinal plants from the Republic of Korea. Explor Res Hypothesis Med 2021; 6(2): 51-59. [CrossRef] google scholar
  • 18. Abdullahi A, Hamzah RU, Jigam AA, Yahya A, Kabiru AY, Muham-mad H, et al. Inhibitory activity of xanthine oxidase by fractions Crateva adansonii. J Acute Dis 2012; 1(2): 126-9. [CrossRef] google scholar
  • 19. Van Slyke DD, Archibald RM. Manometrie, titrimetric and colori-metric methods for measurements of urease activity. J Biol Chem 1944; 154: 623-42. [CrossRef] google scholar
  • 20. Ganai SA, Sheikh FA, Baba ZA, Mir MA, Mantoo MA, Yatoo MA. Anticancer activity of the plant flavonoid luteolin against preclin-ical models of various cancers and insights on different signalling mechanisms modulated. Phytother Res 2021 Feb 13. [Epub ahead of print]. [CrossRef] google scholar
  • 21. Karaman K, Polat B, Ozturk I, Sagdic O, Ozdemir C. Volatile compounds and bioactivity of Eremurus spectabilis (Ciris), a Turkish wild edible vegetable. J Med Food 2011; 14(10): 1238-43. [CrossRef] google scholar
  • 22. Ghasemi S, Xu S, Nabavi SM, Amirkhani MA, Sureda A, Tejada S, et al. Epigenetic targeting of cancer stem cells by polyphenols (can-cer stem cells targeting). Phytother Res 2021 Feb 22. [Epub ahead of print]. [CrossRef] google scholar
  • 23. Karaoglan ES, Gundogdu G, Secme M, Onur S, Miloglu FD, Dodur-ga Y, et al. Eremurus spectabilis, rich source of isoorientin: Isola-tion, quantification and anti-cancer activity on SH-SY5Y neuro-blastoma cells. Curr Pharm Anal 2018; 14(6): 578-85. [CrossRef] google scholar
  • 24. Abubaker SR, Hidayat HJ. Anti tumor potential of local aslerk (Er-emurus spectabilis) leaf extracts by HPLC and applying on cancer cell lines in vitro. Iraqi J Cancer Med Genet 2015; 8(2): 123-8. google scholar
  • 25. Burmaoglu S, Ozcan S, Balcioglu S, Gencel M, Noma SAA, Essiz S, et al. Synthesis, biological evaluation and molecular docking studies of bis-chalcone derivatives as xanthine oxidase inhibitors and an-ticancer agents. Bioorg Chem 2019; 91: 103149. [CrossRef] google scholar
  • 26. Xu H, Li C, Mozziconacci O, Zhu R, Xu Y, Tang Y, et al. Xanthine oxidase-mediated oxidative stress promotes cancer cell-specific apoptosis. Free Radic Biol Med 2019; 139: 70-9. [CrossRef] google scholar
  • 27. Bircan B, Kırbağ S. Determination of antioxidant and antimicrobial properties of Eremurus spectabilis Bieb. Artvin Çoruh Üniv Orman Fak Derg 2015; 16(2): 176-86. [CrossRef] google scholar
  • 28. Symonowicz M, Kolanek M. Flavonoids and their properties to form chelate complexes. Biotechnol Food Sci 2012; 76(1): 35-41. google scholar
  • 29. El Aanachi S, Gali L, Rammali S, Bensouici C, Aassila H, Dari K. In vitro study of the antioxidant, photoprotective, anti-tyrosinase, and anti-urease effects of methanolic extracts from leaves of six Moroccan Lamiaceae. J Food Meas Charact 2021; 15(2): 1785-95. [CrossRef] google scholar
  • 30. Karakaya L, Akgül Y, Nalbantsoy A. Chemical constituents and in vitro biological activities of Eremurus spectabilis leaves. Nat Prod Res 2017; 31(15): 1786-91. [CrossRef] google scholar
  • 31. Mushtaq A, Hussein Masoodi M, Farooq Wali A, Ganai A. Total phe-nolic content, total flavonoid content, in vitro antioxidant activity and antimicrobial activity against human pathogenic bacteria of Eremurus himalaicus-An edible herb of North Western Himalayas. Free Radic Antiox 2017; 7(1): 90-4. [CrossRef] google scholar
  • 32. Nile S, Keum YS, Nile AS, Jalde SS, Patel RV. Antioxidant, anti-in-flammatory, and enzyme inhibitory activity of natural plant fla-vonoids and their synthesized derivatives. J Biochem Mol Toxicol 2017; 32(1): e22002. [CrossRef] google scholar
There are 32 citations in total.

Details

Primary Language English
Subjects Clinical Sciences
Journal Section Research Article
Authors

Bertan Boran Bayrak 0000-0002-0700-5096

Refiye Yanardağ 0000-0003-4185-4363

Publication Date December 8, 2021
Submission Date April 12, 2021
Published in Issue Year 2021

Cite

Vancouver Bayrak BB, Yanardağ R. Histone Deacetylase, Xanthine Oxidase and Urease Inhibitory Activities of Eremurus spectabilisM. Bieb. Extracts. Experimed. 2021;11(3):156-61.