Yıl 2021, Cilt 11 , Sayı 1, Sayfalar 12 - 20 2021-05-03

A Brief Reconnoitre about Effects of MMP9 on Aortic Dissection
MMP9 Geninin Aort Diseksiyonundaki Olası Etkilerinin Araştırılması

Burcu SALMAN YAYLAZ [1] , Melda SARIMAN [2] , Ahmet EKMEKÇİ [3] , Emel ERGÜL [4] , Mahmut ULUGANYAN [5] , Fulya COŞAN [6] , Özgün Melike TOTUK GEDAR [7] , Neslihan ABACI [8]


Objective: Matrix metalloproteinases (MMPs) are the extracellular ma-trix regulators that frequently investigate cardiovascular diseases and cancer metastasis. Our study aimed to examine specific polymorphisms in the MMP9 gene in our patients with aortic dissection and compare the effect of MMP9 on aortic dissection with expression datasets.

Materials and Methods: Q279R and P574R polymorphisms were analyzed in 44 aortic dissection patients and 40 healthy donors via polymerase chain reaction-restriction fragment length polymorphism. (PCR-RFLP) methods. Q279R and P574R prevalence was statistically compared with the medical data of the patients. Additionally, we col-lected datasets of aortic dissection from NCBI GEO to reanalyze GEO2R and RStudio to see metalloproteinase activity on samples. Later, enrich-ment analysis was processed on widely used databases.

Results: Genotypic distribution of alleles was similar in the two study groups. In addition to this, female CG carriers had a higher risk of de-veloping aortic dissection than those of males. As the results of the protein-protein interaction analysis of MMP9 and patients’ clinical data, hypertension was found to be the significant outcome of P574R varia-tion in the patients. In array analysis, MMP9 expression did not change critically, but TIMPs had been downregulated in many samples. Also, MMP9 targeted miRNA expression levels were detected as low in aortic tissue and blood.

Conclusion: Q279R and P574R are two polymorphisms that do not di-rectly affect MMP9 protein structure. Consequently, studied polymor-phisms and performed meta-analysis show that MMP9 does not spark off the phenotype but sets the stage for aortic dissection development as seen in the statistical results. Furthermore, enrichment analysis on datasets shows MMP9 was not a primary reason for vascular remodeling.

Amaç: Kardiyovasküler hastalıklar ve kanser metastazında sıklıkla araştı-rılan matriks metalloproteinazlar (MMPs) ekstraselüler matriks düzenle-yicileridir. Çalışmamız, aort diseksiyonu olan hastalarda MMP9 genindeki spesifik polimorfizmleri incelemeyi ve MMP9'un aort diseksiyonu üzerin-deki etkisini ekspresyon veri setleri ile karşılaştırmayı amaçlamaktadır.

Gereç ve Yöntem: Q279R ve P574R polimorfizmleri 44 aort diseksiyon tanısı almış ve 40 sağlıklı bireyde polimeraz zincir reaksiyonu - restrik-siyon parça uzunluğu polimorfizmi (PCR-RFLP) yöntemiyle çalışıldı. Q279R ve P574R prevalansı istatistiksel olarak hastaların tıbbi verileriyle karşılaştırıldı. Buna ek olarak, NCBI GEO veri tabanından aort diseksiyon veri setleri toplandı ve MMP9 ifadesindeki farklılıkları görmek amacıyla bu veri setleri GEO2R ve RStudio ile yeniden analiz edildi. Elde edilen sonuçların informatik analizi için çevrimiçi veri tabanları kullanıldı.

Bulgular: CG alleli taşıyıcısı kadınların aort diseksiyonu geliştirme riski erkeklerden daha yüksek bulunmasına rağmen her iki çalışma grubun-da da allellerin genotipik dağılımı benzer bulunmuştur. MMP9'un pro-tein-protein etkileşim analizinin ve hastaların tıbbi verilerinin incelen-mesinin sonucu olarak, P574R hipertansiyonu olan hastalarda önemli bir bulgu olarak değerlendirilmiştir. Array verisi analizinde ise MMP9 ifa-desinde kritik bir değişim gözlemlenmemiş olup, birçok örnekte TIMPifade seviyelerinde azalma tespit edilmiştir. Ayrıca MMP9’u hedefleyen miRNA ekspresyon seviyelerinin aort dokusu ve kanda düşük olduğu saptanmıştır.

Sonuç: Q279R ve P574R, MMP9 protein yapısını doğrudan etkilemeyen iki polimorfizmdir. İncelenen polimorfizmler ve gerçekleştirilen meta-a-nalizler, MMP9'un fenotipi doğrudan etkilemediği, ancak istatistiksel sonuçlarda görüldüğü gibi aort diseksiyonu gelişimi için zemin hazır-ladığını göstermektedir.

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Birincil Dil en
Konular Tıp
Bölüm Araştırma Makalesi
Yazarlar

Orcid: 0000-0002-9144-3899
Yazar: Burcu SALMAN YAYLAZ
Kurum: İSTANBUL ÜNİVERSİTESİ, AZİZ SANCAR DENEYSEL TIP ARAŞTIRMA ENSTİTÜSÜ
Ülke: Turkey


Orcid: 0000-0003-0898-529X
Yazar: Melda SARIMAN
Kurum: İSTİNYE ÜNİVERSİTESİ
Ülke: Turkey


Orcid: 0000-0001-5424-149X
Yazar: Ahmet EKMEKÇİ
Kurum: İSTANBUL MEDİCAL PARK HASTANELERİ
Ülke: Turkey


Orcid: 0000-0003-0473-4020
Yazar: Emel ERGÜL
Kurum: KOCAELİ ÜNİVERSİTESİ
Ülke: Turkey


Orcid: 0000-0002-4578-4537
Yazar: Mahmut ULUGANYAN
Kurum: BEZM-İ ÂLEM VAKIF ÜNİVERSİTESİ
Ülke: Turkey


Orcid: 0000-0002-5630-8640
Yazar: Fulya COŞAN
Kurum: BAHÇEŞEHİR ÜNİVERSİTESİ
Ülke: Turkey


Orcid: 0000-0003-1863-6501
Yazar: Özgün Melike TOTUK GEDAR
Kurum: BAHÇEŞEHİR ÜNİVERSİTESİ
Ülke: Turkey


Orcid: 0000-0002-9962-4010
Yazar: Neslihan ABACI (Sorumlu Yazar)
Kurum: İSTANBUL ÜNİVERSİTESİ, AZİZ SANCAR DENEYSEL TIP ARAŞTIRMA ENSTİTÜSÜ
Ülke: Turkey


Destekleyen Kurum İstanbul Üniversitesi Bilimsel Araştırma Projeleri Birimi
Proje Numarası 23468
Tarihler

Başvuru Tarihi : 9 Şubat 2021
Kabul Tarihi : 11 Mart 2021
Yayımlanma Tarihi : 3 Mayıs 2021

Bibtex @araştırma makalesi { experimed874159, journal = {Experimed}, issn = {}, eissn = {2667-5846}, address = {}, publisher = {İstanbul Üniversitesi}, year = {2021}, volume = {11}, pages = {12 - 20}, doi = {10.26650/experimed.2021.874159}, title = {A Brief Reconnoitre about Effects of MMP9 on Aortic Dissection}, key = {cite}, author = {Salman Yaylaz, Burcu and Sarıman, Melda and Ekmekçi, Ahmet and Ergül, Emel and Uluganyan, Mahmut and Coşan, Fulya and Totuk Gedar, Özgün Melike and Abacı, Neslihan} }
APA Salman Yaylaz, B , Sarıman, M , Ekmekçi, A , Ergül, E , Uluganyan, M , Coşan, F , Totuk Gedar, Ö , Abacı, N . (2021). A Brief Reconnoitre about Effects of MMP9 on Aortic Dissection . Experimed , 11 (1) , 12-20 . DOI: 10.26650/experimed.2021.874159
MLA Salman Yaylaz, B , Sarıman, M , Ekmekçi, A , Ergül, E , Uluganyan, M , Coşan, F , Totuk Gedar, Ö , Abacı, N . "A Brief Reconnoitre about Effects of MMP9 on Aortic Dissection" . Experimed 11 (2021 ): 12-20 <https://dergipark.org.tr/tr/pub/experimed/issue/62231/874159>
Chicago Salman Yaylaz, B , Sarıman, M , Ekmekçi, A , Ergül, E , Uluganyan, M , Coşan, F , Totuk Gedar, Ö , Abacı, N . "A Brief Reconnoitre about Effects of MMP9 on Aortic Dissection". Experimed 11 (2021 ): 12-20
RIS TY - JOUR T1 - A Brief Reconnoitre about Effects of MMP9 on Aortic Dissection AU - Burcu Salman Yaylaz , Melda Sarıman , Ahmet Ekmekçi , Emel Ergül , Mahmut Uluganyan , Fulya Coşan , Özgün Melike Totuk Gedar , Neslihan Abacı Y1 - 2021 PY - 2021 N1 - doi: 10.26650/experimed.2021.874159 DO - 10.26650/experimed.2021.874159 T2 - Experimed JF - Journal JO - JOR SP - 12 EP - 20 VL - 11 IS - 1 SN - -2667-5846 M3 - doi: 10.26650/experimed.2021.874159 UR - https://doi.org/10.26650/experimed.2021.874159 Y2 - 2021 ER -
EndNote %0 Experimed A Brief Reconnoitre about Effects of MMP9 on Aortic Dissection %A Burcu Salman Yaylaz , Melda Sarıman , Ahmet Ekmekçi , Emel Ergül , Mahmut Uluganyan , Fulya Coşan , Özgün Melike Totuk Gedar , Neslihan Abacı %T A Brief Reconnoitre about Effects of MMP9 on Aortic Dissection %D 2021 %J Experimed %P -2667-5846 %V 11 %N 1 %R doi: 10.26650/experimed.2021.874159 %U 10.26650/experimed.2021.874159
ISNAD Salman Yaylaz, Burcu , Sarıman, Melda , Ekmekçi, Ahmet , Ergül, Emel , Uluganyan, Mahmut , Coşan, Fulya , Totuk Gedar, Özgün Melike , Abacı, Neslihan . "A Brief Reconnoitre about Effects of MMP9 on Aortic Dissection". Experimed 11 / 1 (Mayıs 2021): 12-20 . https://doi.org/10.26650/experimed.2021.874159
AMA Salman Yaylaz B , Sarıman M , Ekmekçi A , Ergül E , Uluganyan M , Coşan F , Totuk Gedar Ö , Abacı N . A Brief Reconnoitre about Effects of MMP9 on Aortic Dissection. Experimed. 2021; 11(1): 12-20.
Vancouver Salman Yaylaz B , Sarıman M , Ekmekçi A , Ergül E , Uluganyan M , Coşan F , Totuk Gedar Ö , Abacı N . A Brief Reconnoitre about Effects of MMP9 on Aortic Dissection. Experimed. 2021; 11(1): 12-20.
IEEE B. Salman Yaylaz , M. Sarıman , A. Ekmekçi , E. Ergül , M. Uluganyan , F. Coşan , Ö. Totuk Gedar ve N. Abacı , "A Brief Reconnoitre about Effects of MMP9 on Aortic Dissection", Experimed, c. 11, sayı. 1, ss. 12-20, May. 2021, doi:10.26650/experimed.2021.874159