Yıl 2021, Cilt 11 , Sayı 1, Sayfalar 33 - 37 2021-05-03

B Hücre Reseptör (BHR) Sinyal Yolağı Faktörlerinin Ekspresyon Düzeyleri Klinik İzole Sendromdan Multipl Skleroza Dönüşümle İlişkilidir
Expression Levels of B Cell Receptor (BCR) Signaling Pathway Factors are Associated with Conversion from Clinically Isolated Syndrome to Multiple Sclerosis

Zerrin KARAASLAN [1] , Ece AKBAYIR [2] , Elif ŞANLI [3] , Melis ŞEN [4] , Recai TÜRKOĞLU [5] , Cem İsmail KÜÇÜKALİ [6] , Erdem TÜZÜN [7]


Amaç: Daha önceki çalışmalarımızda, multipl skleroza (MS) dö-nüşen klinik izole sendrom (KİS) olgularının ilk atakta alınan kan örneklerinde periferik kan B hücre düzeylerinin değiştiğini göste-rilmiştir. Bu çalışmada beş yıllık takipte MS’e dönüşen ve dönüşme-yen KİS olgularında B hücre reseptör (BHR) ilişkili genlerin ekspres-yon düzeylerinin araştırılması amaçlanmıştır.

Yöntem: Yedi MS’e dönüşen, 12 MS’e dönüşmeyen KİS olgusu ile yaş ve cinsiyet açısından eşleştirilmiş 10 sağlıklı donör çalışmaya dahil edildi. İlk atak döneminde elde edilen donmuş periferik kan mononükleer hücrelerinden (PKMH) RNA izolasyon kiti kullanılarak total RNAlar izole edildi. Örneklerden gerçek zamanlı polimeraz zincir reaksiyonu (RT-PCR) yöntemi ile ankrin tekrarlı B-hücre adap-tör proteini (BANK1), B lenfosit kinaz (BLK), Fc reseptör benzeri pro-tein 2 (FCRL2) genlerinin ekspresyonları değerlendirildi.

Bulgular: MS’e dönüşmeyen KİS olgularında BANK1, BLK ve FCRL2 gen ekspresyon düzeyleri MS’e dönüşen KİS olguları ve sağlıklı kontrollere kıyasla artmış olarak saptandı.

Sonuç: BHR sinyalizasyonunu inhibe eden gen ekspresyonlarının MS’e dönüşmeyen olgularda artması, abartılı B hücre yanıtının B hücre proliferasyonunun optimize edilmesi yoluyla baskılanması-nın KİS’ten MS’e ilerleyişi engelleyebileceğini düşündürmüştür.

Objective: We have previously shown altered blood B cells in the first attack blood samples of clinically isolated syndrome (CIS) pa-tients, who converted to multiple sclerosis (MS). Our aim was to investigate levels of B cell receptor (BCR)-associated genes expres-sion in converting CIS (CIS-c) and non-converting CIS patients (CIS-nc) with a 5-year follow-up.

Methods: Seven CIS-c patients, 12 CIS-nc patients and 10 age-sex matched healthy donors were enrolled in this study. RNA was extracted from frozen peripheral blood cells of subjects that were obtained in the first attack using a RNA isolation kit. Expression levels of B-cell adaptor protein with ankyrin repeats 1 (BANK1), B lymphocyte kinase (BLK) and Fc receptor-like protein 2 (FCRL2) genes were evaluated samples by real-time polymerase chain re-action (RT-PCR).

Results: CIS-nc patients showed elevated BANK1, BLK and FCRL2 gene expression levels as compared to CIS-c patients and healthy controls.

Conclusions: Expression levels of the genes that inhibit BCR signal-ing are increased in CIS-nc, suggesting that inhibiting exaggerated B cell response by optimization of B cell proliferation may prevent the progression of clinically isolated syndrome to MS.

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Birincil Dil en
Konular Tıp
Bölüm Araştırma Makalesi
Yazarlar

Orcid: 0000-0002-5784-1324
Yazar: Zerrin KARAASLAN (Sorumlu Yazar)
Kurum: İSTANBUL ÜNİVERSİTESİ, AZİZ SANCAR DENEYSEL TIP ARAŞTIRMA ENSTİTÜSÜ
Ülke: Turkey


Orcid: 0000-0002-8148-6940
Yazar: Ece AKBAYIR
Kurum: İSTANBUL ÜNİVERSİTESİ, AZİZ SANCAR DENEYSEL TIP ARAŞTIRMA ENSTİTÜSÜ
Ülke: Turkey


Orcid: 0000-0002-9593-7394
Yazar: Elif ŞANLI
Kurum: İSTANBUL ÜNİVERSİTESİ, AZİZ SANCAR DENEYSEL TIP ARAŞTIRMA ENSTİTÜSÜ
Ülke: Turkey


Orcid: 0000-0002-3557-8203
Yazar: Melis ŞEN
Kurum: İSTANBUL ÜNİVERSİTESİ, AZİZ SANCAR DENEYSEL TIP ARAŞTIRMA ENSTİTÜSÜ
Ülke: Turkey


Orcid: 0000-0001-7685-4633
Yazar: Recai TÜRKOĞLU
Kurum: İSTANBUL HAYDARPAŞA NUMUNE SAĞLIK UYGULAMA VE ARAŞTIRMA MERKEZİ
Ülke: Turkey


Orcid: 0000-0001-9851-8577
Yazar: Cem İsmail KÜÇÜKALİ
Kurum: İSTANBUL ÜNİVERSİTESİ, AZİZ SANCAR DENEYSEL TIP ARAŞTIRMA ENSTİTÜSÜ
Ülke: Turkey


Orcid: 0000-0002-4483-0394
Yazar: Erdem TÜZÜN
Kurum: İSTANBUL ÜNİVERSİTESİ, AZİZ SANCAR DENEYSEL TIP ARAŞTIRMA ENSTİTÜSÜ
Ülke: Turkey


Tarihler

Başvuru Tarihi : 8 Mart 2021
Kabul Tarihi : 29 Mart 2021
Yayımlanma Tarihi : 3 Mayıs 2021

Bibtex @araştırma makalesi { experimed888583, journal = {Experimed}, issn = {}, eissn = {2667-5846}, address = {}, publisher = {İstanbul Üniversitesi}, year = {2021}, volume = {11}, pages = {33 - 37}, doi = {10.26650/experimed.2021.888583}, title = {Expression Levels of B Cell Receptor (BCR) Signaling Pathway Factors are Associated with Conversion from Clinically Isolated Syndrome to Multiple Sclerosis}, key = {cite}, author = {Karaaslan, Zerrin and Akbayır, Ece and Şanlı, Elif and Şen, Melis and Türkoğlu, Recai and Küçükali, Cem İsmail and Tüzün, Erdem} }
APA Karaaslan, Z , Akbayır, E , Şanlı, E , Şen, M , Türkoğlu, R , Küçükali, C , Tüzün, E . (2021). Expression Levels of B Cell Receptor (BCR) Signaling Pathway Factors are Associated with Conversion from Clinically Isolated Syndrome to Multiple Sclerosis . Experimed , 11 (1) , 33-37 . DOI: 10.26650/experimed.2021.888583
MLA Karaaslan, Z , Akbayır, E , Şanlı, E , Şen, M , Türkoğlu, R , Küçükali, C , Tüzün, E . "Expression Levels of B Cell Receptor (BCR) Signaling Pathway Factors are Associated with Conversion from Clinically Isolated Syndrome to Multiple Sclerosis" . Experimed 11 (2021 ): 33-37 <https://dergipark.org.tr/tr/pub/experimed/issue/62231/888583>
Chicago Karaaslan, Z , Akbayır, E , Şanlı, E , Şen, M , Türkoğlu, R , Küçükali, C , Tüzün, E . "Expression Levels of B Cell Receptor (BCR) Signaling Pathway Factors are Associated with Conversion from Clinically Isolated Syndrome to Multiple Sclerosis". Experimed 11 (2021 ): 33-37
RIS TY - JOUR T1 - Expression Levels of B Cell Receptor (BCR) Signaling Pathway Factors are Associated with Conversion from Clinically Isolated Syndrome to Multiple Sclerosis AU - Zerrin Karaaslan , Ece Akbayır , Elif Şanlı , Melis Şen , Recai Türkoğlu , Cem İsmail Küçükali , Erdem Tüzün Y1 - 2021 PY - 2021 N1 - doi: 10.26650/experimed.2021.888583 DO - 10.26650/experimed.2021.888583 T2 - Experimed JF - Journal JO - JOR SP - 33 EP - 37 VL - 11 IS - 1 SN - -2667-5846 M3 - doi: 10.26650/experimed.2021.888583 UR - https://doi.org/10.26650/experimed.2021.888583 Y2 - 2021 ER -
EndNote %0 Experimed Expression Levels of B Cell Receptor (BCR) Signaling Pathway Factors are Associated with Conversion from Clinically Isolated Syndrome to Multiple Sclerosis %A Zerrin Karaaslan , Ece Akbayır , Elif Şanlı , Melis Şen , Recai Türkoğlu , Cem İsmail Küçükali , Erdem Tüzün %T Expression Levels of B Cell Receptor (BCR) Signaling Pathway Factors are Associated with Conversion from Clinically Isolated Syndrome to Multiple Sclerosis %D 2021 %J Experimed %P -2667-5846 %V 11 %N 1 %R doi: 10.26650/experimed.2021.888583 %U 10.26650/experimed.2021.888583
ISNAD Karaaslan, Zerrin , Akbayır, Ece , Şanlı, Elif , Şen, Melis , Türkoğlu, Recai , Küçükali, Cem İsmail , Tüzün, Erdem . "Expression Levels of B Cell Receptor (BCR) Signaling Pathway Factors are Associated with Conversion from Clinically Isolated Syndrome to Multiple Sclerosis". Experimed 11 / 1 (Mayıs 2021): 33-37 . https://doi.org/10.26650/experimed.2021.888583
AMA Karaaslan Z , Akbayır E , Şanlı E , Şen M , Türkoğlu R , Küçükali C , Tüzün E . Expression Levels of B Cell Receptor (BCR) Signaling Pathway Factors are Associated with Conversion from Clinically Isolated Syndrome to Multiple Sclerosis. Experimed. 2021; 11(1): 33-37.
Vancouver Karaaslan Z , Akbayır E , Şanlı E , Şen M , Türkoğlu R , Küçükali C , Tüzün E . Expression Levels of B Cell Receptor (BCR) Signaling Pathway Factors are Associated with Conversion from Clinically Isolated Syndrome to Multiple Sclerosis. Experimed. 2021; 11(1): 33-37.
IEEE Z. Karaaslan , E. Akbayır , E. Şanlı , M. Şen , R. Türkoğlu , C. Küçükali ve E. Tüzün , "Expression Levels of B Cell Receptor (BCR) Signaling Pathway Factors are Associated with Conversion from Clinically Isolated Syndrome to Multiple Sclerosis", Experimed, c. 11, sayı. 1, ss. 33-37, May. 2021, doi:10.26650/experimed.2021.888583