İmplant Yüzey Özelliği Seçimi Adherent Hücrelerin Canlılığını Etkileyebilir

Year 2021, Volume: 11 Issue: 1, 38 - 44, 03.05.2021

Gül İpek Gündoğan , Tayfun Cıvak , Cenk Kığ

https://doi.org/10.26650/experimed.2021.898858

Abstract

Amaç: İmplant materyallerinin hücre canlılığı ve hücre adezyo-nu üzerindeki in vitro etkilerinin değerlendirilmesi, implantların biyo-uyumluluklarının tahmin edilmesi yönünde değerli bilgiler sağlayabilir. Bu nedenle, basit ve maliyetli olmayan bir yöntem kul-lanılarak, farklı yüzey özelliklerine sahip implantların hücre canlılığı ve adezyonunu etkileyip etkilemeyeceğinin araştırılması amaçlanmıştır.

Gereç ve Yöntemler: Farklı diş implant yüzeylerinin (anotlanmış (AN), patlatılmış buruşuk (BW), kum/asitle aşındırılmış (GA), püs-kürtülmüş hidroksi apatit yüzey (HB)) yapışma ve canlılık üzerin-deki etkileri, adherent insan osteoblast hücre soyunda agar bazlı bir in vitro teknik kullanılarak karşılaştırılmıştır. Hücre canlılıklarının belirlenmesinde, 3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolium bromid (MTT) ve tripan mavisi boyama teknikleri uygulanmıştır.

Bulgular: Hücre adezyonunun, dental implantların yüzey özellik-lerindeki farklılıklardan önemli ölçüde etkilenmediği görülmüştür (AN: 78,21±0,52; BW: 78,22±0,48; GA: 78,44±0,85; HB: 77,26±0,96). Ancak, diş implantlarının yüzey özelliklerinin, implantlara tutunan hücrelerin canlılığı üzerinde etkisi olduğu tespit edilmiştir. Tutu-nan hücrelerin canlılığının, HB yüzeyine kıyasla AN, BW, GA yüzeylerinde anlamlı olarak daha yüksek olduğu bulunmuştur (AN: 72,28±6,04, BW: 67,02±3,47, GA: 85,82±5,05 ve HB: 27,98±10,47).

Sonuçlar: Çalışma kapsamında yapılan in vitro deneylerden elde edilen bulgular; AN, BW, GA yüzeylerinin adherent hücrelerin canlı-lığını korumaları için HB yüzeylerinden daha iyi bir platform sağla-yabileceğini göstermektedir.

Keywords

Biyouyumlu yüzeyler, düzensiz yüzeyler, hücre adezyon potansiyeli, hücre kültürü, dental implantlar

Choice of Implant Surface-Feature May Affect the Viability of the Adherent Cells

Abstract

Objective:In vitro evaluation of implant materials' effects on cell adhesion and viability can provide useful information for predict-ing implant biocompatibility. Therefore by using a simple and in-expensive method, it was aimed to investigate whether different implant surface-features might have distinct effects on the viabili-ty and adherence of the cells.

Material and Methods: Different dental implant surfaces (anod-ized (AN), blasted wrinkled (BW), grit/acid etched (GA), and hy-droxylapatite sprayed (HB)) were tested for their possible effects on adhesion and viability of the adherent human osteoblast cells by using an agar-based in vitro technique. Viability of the cells was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and trypan blue staining.

Results: The rate of cell adhesion did not seem to be significantly affected by the differences in surface features of dental implants (AN:78.21±0.52; BW:78.22±0.48; GA:78.44±0.85; HB:77.26±0.96). The surface features of the dental implants had an impact on the viability of the attached cells on the implants. Viability of the at-tached cells was significantly higher on AN, BW, GA surfaces when compared to the HB surface (AN: 72.28±6.04, BW: 67.02±3.47, GA: 85.82±5.05, and HB: 27.98±10.47).

Conclusions:In vitro findings suggests that AN, BW, GA surfaces may provide a better platform than HB surfaces to maintain the viability of bound cells.

Keywords

Biocompatible surfaces, irregular surfaces, cell adhesion potential, cell culture, dental implants

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