Yıl 2021, Cilt 11 , Sayı 1, Sayfalar 38 - 44 2021-05-03

Choice of Implant Surface-Feature May Affect the Viability of the Adherent Cells
İmplant Yüzey Özelliği Seçimi Adherent Hücrelerin Canlılığını Etkileyebilir

Gül İpek GÜNDOĞAN [1] , Tayfun CIVAK [2] , Cenk KIĞ [3]


Objective:In vitro evaluation of implant materials' effects on cell adhesion and viability can provide useful information for predict-ing implant biocompatibility. Therefore by using a simple and in-expensive method, it was aimed to investigate whether different implant surface-features might have distinct effects on the viabili-ty and adherence of the cells.

Material and Methods: Different dental implant surfaces (anod-ized (AN), blasted wrinkled (BW), grit/acid etched (GA), and hy-droxylapatite sprayed (HB)) were tested for their possible effects on adhesion and viability of the adherent human osteoblast cells by using an agar-based in vitro technique. Viability of the cells was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and trypan blue staining.

Results: The rate of cell adhesion did not seem to be significantly affected by the differences in surface features of dental implants (AN:78.21±0.52; BW:78.22±0.48; GA:78.44±0.85; HB:77.26±0.96). The surface features of the dental implants had an impact on the viability of the attached cells on the implants. Viability of the at-tached cells was significantly higher on AN, BW, GA surfaces when compared to the HB surface (AN: 72.28±6.04, BW: 67.02±3.47, GA: 85.82±5.05, and HB: 27.98±10.47).

Conclusions:In vitro findings suggests that AN, BW, GA surfaces may provide a better platform than HB surfaces to maintain the viability of bound cells.

Amaç: İmplant materyallerinin hücre canlılığı ve hücre adezyo-nu üzerindeki in vitro etkilerinin değerlendirilmesi, implantların biyo-uyumluluklarının tahmin edilmesi yönünde değerli bilgiler sağlayabilir. Bu nedenle, basit ve maliyetli olmayan bir yöntem kul-lanılarak, farklı yüzey özelliklerine sahip implantların hücre canlılığı ve adezyonunu etkileyip etkilemeyeceğinin araştırılması amaçlanmıştır.

Gereç ve Yöntemler: Farklı diş implant yüzeylerinin (anotlanmış (AN), patlatılmış buruşuk (BW), kum/asitle aşındırılmış (GA), püs-kürtülmüş hidroksi apatit yüzey (HB)) yapışma ve canlılık üzerin-deki etkileri, adherent insan osteoblast hücre soyunda agar bazlı bir in vitro teknik kullanılarak karşılaştırılmıştır. Hücre canlılıklarının belirlenmesinde, 3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolium bromid (MTT) ve tripan mavisi boyama teknikleri uygulanmıştır.

Bulgular: Hücre adezyonunun, dental implantların yüzey özellik-lerindeki farklılıklardan önemli ölçüde etkilenmediği görülmüştür (AN: 78,21±0,52; BW: 78,22±0,48; GA: 78,44±0,85; HB: 77,26±0,96). Ancak, diş implantlarının yüzey özelliklerinin, implantlara tutunan hücrelerin canlılığı üzerinde etkisi olduğu tespit edilmiştir. Tutu-nan hücrelerin canlılığının, HB yüzeyine kıyasla AN, BW, GA yüzeylerinde anlamlı olarak daha yüksek olduğu bulunmuştur (AN: 72,28±6,04, BW: 67,02±3,47, GA: 85,82±5,05 ve HB: 27,98±10,47).

Sonuçlar: Çalışma kapsamında yapılan in vitro deneylerden elde edilen bulgular; AN, BW, GA yüzeylerinin adherent hücrelerin canlı-lığını korumaları için HB yüzeylerinden daha iyi bir platform sağla-yabileceğini göstermektedir.

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Birincil Dil en
Konular Tıp
Bölüm Araştırma Makalesi
Yazarlar

Orcid: 0000-0002-9438-6113
Yazar: Gül İpek GÜNDOĞAN (Sorumlu Yazar)
Kurum: İSTANBUL YENİ YÜZYIL ÜNİVERSİTESİ
Ülke: Turkey


Orcid: 0000-0002-8770-3731
Yazar: Tayfun CIVAK
Kurum: İSTANBUL YENİ YÜZYIL ÜNİVERSİTESİ
Ülke: Turkey


Orcid: 0000-0002-6318-5001
Yazar: Cenk KIĞ
Kurum: İSTANBUL YENİ YÜZYIL ÜNİVERSİTESİ
Ülke: Turkey


Tarihler

Başvuru Tarihi : 17 Mart 2021
Kabul Tarihi : 2 Nisan 2021
Yayımlanma Tarihi : 3 Mayıs 2021

Bibtex @araştırma makalesi { experimed898858, journal = {Experimed}, issn = {}, eissn = {2667-5846}, address = {}, publisher = {İstanbul Üniversitesi}, year = {2021}, volume = {11}, pages = {38 - 44}, doi = {10.26650/experimed.2021.898858}, title = {Choice of Implant Surface-Feature May Affect the Viability of the Adherent Cells}, key = {cite}, author = {Gündoğan, Gül İpek and Cıvak, Tayfun and Kığ, Cenk} }
APA Gündoğan, G , Cıvak, T , Kığ, C . (2021). Choice of Implant Surface-Feature May Affect the Viability of the Adherent Cells . Experimed , 11 (1) , 38-44 . DOI: 10.26650/experimed.2021.898858
MLA Gündoğan, G , Cıvak, T , Kığ, C . "Choice of Implant Surface-Feature May Affect the Viability of the Adherent Cells" . Experimed 11 (2021 ): 38-44 <https://dergipark.org.tr/tr/pub/experimed/issue/62231/898858>
Chicago Gündoğan, G , Cıvak, T , Kığ, C . "Choice of Implant Surface-Feature May Affect the Viability of the Adherent Cells". Experimed 11 (2021 ): 38-44
RIS TY - JOUR T1 - Choice of Implant Surface-Feature May Affect the Viability of the Adherent Cells AU - Gül İpek Gündoğan , Tayfun Cıvak , Cenk Kığ Y1 - 2021 PY - 2021 N1 - doi: 10.26650/experimed.2021.898858 DO - 10.26650/experimed.2021.898858 T2 - Experimed JF - Journal JO - JOR SP - 38 EP - 44 VL - 11 IS - 1 SN - -2667-5846 M3 - doi: 10.26650/experimed.2021.898858 UR - https://doi.org/10.26650/experimed.2021.898858 Y2 - 2021 ER -
EndNote %0 Experimed Choice of Implant Surface-Feature May Affect the Viability of the Adherent Cells %A Gül İpek Gündoğan , Tayfun Cıvak , Cenk Kığ %T Choice of Implant Surface-Feature May Affect the Viability of the Adherent Cells %D 2021 %J Experimed %P -2667-5846 %V 11 %N 1 %R doi: 10.26650/experimed.2021.898858 %U 10.26650/experimed.2021.898858
ISNAD Gündoğan, Gül İpek , Cıvak, Tayfun , Kığ, Cenk . "Choice of Implant Surface-Feature May Affect the Viability of the Adherent Cells". Experimed 11 / 1 (Mayıs 2021): 38-44 . https://doi.org/10.26650/experimed.2021.898858
AMA Gündoğan G , Cıvak T , Kığ C . Choice of Implant Surface-Feature May Affect the Viability of the Adherent Cells. Experimed. 2021; 11(1): 38-44.
Vancouver Gündoğan G , Cıvak T , Kığ C . Choice of Implant Surface-Feature May Affect the Viability of the Adherent Cells. Experimed. 2021; 11(1): 38-44.
IEEE G. Gündoğan , T. Cıvak ve C. Kığ , "Choice of Implant Surface-Feature May Affect the Viability of the Adherent Cells", Experimed, c. 11, sayı. 1, ss. 38-44, May. 2021, doi:10.26650/experimed.2021.898858