Testis Mikrobiyotasının Erkek İnfertilitesi ile İlişkisi

Yıl 2021, Cilt 11, Sayı 2, 140 - 142, 25.08.2021

Ayşe ALTUN Sibel BULGURCUOGLU KURAN İlknur KESKİN Ateş KADIOĞLU

https://doi.org/10.26650/experimed.2021.955321

Öz

Son yıllarda mikrobiyotanın insan sağlığı üzerindeki etkileri hak-kında önemli bulgular rapor edilmiştir. Çalışmamızda erkek inferti-litesi olan 2 hastadan testiküler sperm ekstraksiyonu (TESE) ile elde edilen testis dokusunun mikrobiyota analizinin sonuçlarını karşı-laştırmayı amaçladık. Azospermik olan ilk hastada, toplanan doku homojenatlarında Illumina dizilemesi ile şu bakteri florasına sap-tandı: Firmicutes (%53), Bacteroidetes (%12), Actinobacteria (%12), Proteobacteria (%8), Fusobacteria (%8), SR1 (%7), TM7-3 (%3) ve OD-1 (%1). Oligozoospermi olan ikinci hastanın ise testis dokusun-da Firmicutes (%13), Bacteroidetes (%15), Actinobacteria (%7) ve Proteobacteria (%64) bakterileri saptandı. Ön çalışmamızda, infer-til erkeklerin testis dokusunda belirli bakteri türlerinin bulunduğu doğrulandı. Erkek infertilitesinin patofizyolojisinde bu mikroorga-nizmaların önemini anlamak için infertil ve sağlıklı erkeklerle yapı-lacak ileri çalışmalara ihtiyaç vardır.

Anahtar Kelimeler

Testiküler doku, mikrobiyota, erkek infertilitesi

The Association Between the Testis Microbiota and Male Infertility

Öz

In recent years, substantial findings have been reported about the effects of microbiota on human health. In our study, we aimed to compare the outcomes of microbiota assays of testicular tissue ob-tained by testicular sperm extraction (TESE) from 2 patients with male infertility. In the first patient who had azoospermia, Illumina sequencing detected the following bacterial flora in the collected tissue homogenates: Firmicutes (53%), Bacteroidetes (12%), Ac-tinobacteria (12%), Proteobacteria (8%), Fusobacteria (8%), SR1 (7%), TM7-3 (3%) and OD-1 (1%). In the second patient who had oligozoospermia, Firmicutes (13%), Bacteroidetes (15%), Actino-bacteria (7%) and Proteobacteria (64%) were detected in the tes-ticular tissue. Our preliminary study confirmed that certain types of bacteria exist in testicular tissues of infertile men. Future studies with more infertile and healthy men are required to understand the importance of these microorganisms in the pathophysiology of male infertility.

Anahtar Kelimeler

Testicular tissue, microbiota, male infertility

Kaynakça

• 1. Gevers D, Knight R, Petrosino JF, Huang K, McGuire AL, Birren BW, et al. The Human Microbiome Project: A community resource for the healthy human microbiome. PLoS Biol 2012; 10(8): e1001377. google scholar
• 2. Blaser M, Bork P, Fraser C, Knight R, Wang J. The microbiome ex-plored: recent insights and future challenges. Nat Rev Microbiol 2013; 11: 213-7. [CrossRef] google scholar
• 3. Alfano M, Ferrarese R, Locatelli I, Ventimiglia E, Ippolito S, Gallina P, et al. Testicular microbiome in azoospermic men: first evidence of the impact of an altered microenvironment. Hum Reprod 2018; 33: 1212-7. [CrossRef] google scholar
• 4. Markle JG, Frank DN, Mortin-Toth S, Robertson CE, Feazel LM, Rolle-Kampczyk U, et al. Sex differences in the gut microbiome drive hormone-dependent regulation of autoimmunity. Science 2013; 339: 1084-8. [CrossRef] google scholar
• 5. Magoc T, Salzberg SL. FLASH: fast length adjustment of short reads to improve genome assemblies. Bioinformatics 2011; 27: 2957-63. [CrossRef] google scholar
• 6. Caporaso JG, Lauber CL, Walters WA, Berg-Lyons D, Huntley J, Fi-erer N, et al. Ultra-high-throughput microbial community analy-sis on the Illumina HiSeq and MiSeq platforms. ISME J 2012; 6(8): 1621-4. [CrossRef] google scholar
• 7. Barazani Y, Katz BF, Nagler HM, Stember DS. Lifestyle, environ-ment, and male reproductive health. Urol Clin North Am 2014; 41: 55-66. [CrossRef] google scholar
• 8. Vogl AW, Soucy LJ, Foo V. Ultrastructure of Sertoli-cell penetrat-ing processes found in germ cells of the golden-mantled ground squirrel (spermophilus lateralis). Am J Anat 1985; 172: 75-86. [CrossRef] google scholar
• 9. Hai Y, Hou J, Liu Y, Liu Y, Yang H, Li Z, et al. The roles and regulation of Sertoli cells in fate determinations of spermatogonial stem cells and spermatogenesis. Semin Cell Dev Biol 2014; 29: 66-75. [CrossRef] google scholar
• 10. Ryu BY, Orwig KE, Oatley JM, Avarbock MR, Brinster RL. Effects of aging and niche microenvironment on spermatogonial stem cell self-renewal. Stem Cells 2006; 24: 1505-11. [CrossRef] google scholar
• 11. Hou D, Zhou X, Zhong X, Settles ML, Herring J, Wang L, et al. Mi-crobiota of the seminal fluid from healthy and infertile men. Fertil Steril 2013; 100: 1261-69. [CrossRef] google scholar
• 12. Weng SL, Chiu CM, Lin FM, Huang WC, Liang C, Yang T, et al. Bacte-rial communities in semen from men of infertile couples: metage-nomic sequencing reveals relationships of seminal microbiota to semen quality. PLoS One 2014; 9: e110152. google scholar