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Dye Ligand Affinity Nanoparticles for the Depletion of Biomolecules in Proteomics

Yıl 2022, Cilt: 12 Sayı: 1, 18 - 23, 18.03.2022
https://doi.org/10.26650/experimed.2022.1058018

Öz

Objective: Serum proteins are indicators for certain diseases. However, detection of the biomarkers is difficult because the more abundant proteins mask the less abundant ones. The depletion of abundant serum proteins will help in the discovery and detection of less abundant proteins that may prove to be informative disease markers. Dye ligands have attracted great attention because of their cost-effectiveness, easy immobilization, stability, and high binding capacity. Due to these advantageous properties, dye ligands have also been chosen as an alternative for biological ligands such as antibodies, enzymes, etc.

Materials and Methods: Poly (ethylene glycol dimethacrylate) [p (EGDM)] nanoparticles were prepared using the surfactant-free emulsion polymerization technique. Then, Reactive Red 120 (RR 120) dye was immobilized to nanoparticles in a nucleophilic substitution reaction. The RR120 attached nanoparticles were characterized.

Results: The size/size distribution of p (EGDM) nanoparticles was measured with a Zeta sizer. The scanning electron microscope (SEM) images were found to support a measurement of around 100 nm. The maximum adsorbed amount of albumin was observed at pH 6.0. The maximum depleted albumin concentration was found to be 453.9 mg/g nanoparticles according to the experimental results. Desorption studies were carried out by addition of 0.5 M of KSCN to the albumin solutions. The desorption results showed that the binding of albumin to the nanoparticle was reversible.

Conclusion: Our results demonstrated that dye attached nanoparticles have the potential for depleting albumin from serum in proteomics

Destekleyen Kurum

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Proje Numarası

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Teşekkür

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Kaynakça

  • 1. Andaç M, Denizli A. Affinity-recognition-based polymeric cryogels for protein depletion studies. Rsc Advances 2014; 4(59): 31130-41. [CrossRef] google scholar
  • 2. Bellei E, Bergamini S, Monari E, Fantoni LI, Cuoghi A, Ozben T, et al. High-abundance proteins depletion for serum proteomic anal-ysis: concomitant removal of non-targeted proteins. Amino Acids 2011; 40(1): 145-56. [CrossRef] google scholar
  • 3. Göktürk I, Tamahkar E, Yılmaz F, Denizli A. Protein depletion with bacterial cellulose nanofibers. J Chromatogr B 2018; 1099: 1-9. [CrossRef] google scholar
  • 4. Borberg E, Pashko S, Koren V, Burstein L, Patolsky F. Depletion of Highly Abundant Protein Species from Biosamples by the Use of a Branched Silicon Nanopillar On-Chip Platform. Anal Chem 2021; 93(43): 14527-36. [CrossRef] google scholar
  • 5. Siegmund R, Kiehntopf M, Deufel T. (2009). Evaluation of two dif-ferent albumin depletion strategies for improved analysis of hu-man CSF by SELDI-TOF-MS. Clin Biochem 2009; 42(10-11): 113643. [CrossRef] google scholar
  • 6. da Costa JP, Santos PS, Vitorino R, Rocha-Santos T, Duarte AC. How google scholar
  • low can you go? A current perspective on low-abundance pro-teomics. TrAC Trends Anal Chem 2017; 93: 171-82. [CrossRef] google scholar
  • 7. Uygun DA, Akduman B, Uygun M, Akgöl S, Denizli A. Purification of papain using reactive green 5 attached supermacroporous monolithic cryogel. Appl Biochem Biotechnol 2012; 167(3): 55263. [CrossRef] google scholar
  • 8. Akduman B, Uygun M, Çoban EP, Uygun DA, Bıyık H, Akgöl S. Reversible immobilization of urease by using bacterial cellulose nanofibers. Appl Biochem Biotechnol 2013; 171(8): 2285-94. [CrossRef] google scholar
  • 9. Uygun M, Uygun DA, Altunbaş C, Akgöl S, Denizli A. Dye attached nanoparticles for lysozyme adsorption. Sep Sci Technol 2014; 49(8): 1270-8. [CrossRef] google scholar
  • 10. Poddar S, Sharmeen S, Hage DS. Affinity monolith chromatog-raphy: a review of general principles and recent developments. Electrophoresis 2021; 42(24): 2577-98. [CrossRef] google scholar
  • 11. Andac M, Galaev I, Denizli A. Dye attached poly (hydroxyethyl methacrylate) cryogel for albumin depletion from human serum. J Sep Sci 2012; 35(9): 1173-82. [CrossRef] google scholar
  • 12. Wongchuphan R, Tey BT, Tan WS, Taip FS, Kamal SMM, Ling TC. Ap-plication of dye-ligands affinity adsorbent in capturing of rabbit immunoglobulin G. Biochem Eng J 2009; 45(3): 232-8. [CrossRef] google scholar
  • 13. Öztürk N, Tabak A, Akgöl S, Denizli A. Newly synthesized ben-tonite-histidine (Bent-His) micro-composite affinity sorbents for IgG adsorption. Colloids Surf A. Physicochem and Eng Asp 2007; 301(1-3): 490-7. [CrossRef] google scholar
  • 14. Alpay P, Uygun DA. Usage of immobilized papain for enzymatic hydrolysis of proteins. J Mol Catal B: Enzymatic 2015; 111: 56-63. [CrossRef] google scholar
  • 15. Akgöl S, Öztürk N, Denizli A. New generation polymeric nano-spheres for lysozyme adsorption. J Aappl PolymSci 2010;115(3): 1608-15. [CrossRef] google scholar
  • 16. Türkmen D, Öztürk N, Akgöl S, Elkak A, Denizli A. Phenylalanine containing hydrophobic nanospheres for antibody purification. Biotechnol Prog 2008; 24(6): 1297-1303. [CrossRef] google scholar
  • 17. Liao MH, Chen DH. Fast and efficient adsorption/desorption of protein by a novel magnetic nano-adsorbent. Biotechnol Lett 2002; 24(22): 1913-7. [CrossRef] google scholar
  • 18. Hegedûs I, Nagy E. Improvement of chymotrypsin enzyme sta-bility as single enzyme nanoparticles. Chem Eng Sci 2009; 64(5): 1053-60. [CrossRef] google scholar
  • 19. Uygun DA, Çorman ME, Öztürk N, Akgöl S, Denizli A. Poly (hy-droxyethyl methacrylate-co-methacryloylamidotryptophane) nanospheres and their utilization as affinity adsorbents for por-cine pancreas lipase adsorption. Mater Sci and Eng: C 2010; 30(8): 1285-90. [CrossRef] google scholar
  • 20. Yavuz H, Denizli A. Dye affinity hollow fibers for albumin purifica-tion. Macromol Biosci 2004; 4(2): 84-91. [CrossRef] google scholar
  • 21. Altıntaş EB, Denizli A. Efficient removal of albumin from human serum by monosize dye-affinity beads. J Chromatog B 2006; 832(2): 216-23. [CrossRef] google scholar
  • 22. Zhang J, Zhang Z, Song Y, Cai H. Bovine serum albumin (BSA) adsorption with Cibacron Blue F3GA attached chitosan micro-spheres. React Funct Polym 2006; 66(9): 916-23. [CrossRef] google scholar
  • 23. Tuzmen N, Kalburcu T, Uygun DA, Akgol S, Denizli A. A novel af-finity disks for bovine serum albumin purification. Appl Biochem Biotechnol 2015; 175(1): 454-68. [CrossRef] google scholar
  • 24. Orhan H, Evli S, Dabanca MB, Başbülbül G, Uygun M, Uygun DA. Bacteria killer enzyme attached magnetic nanoparticles. Mater Sci Eng C Mater Biol Appl 2019; 94: 558-64. [CrossRef] google scholar
  • 25. Kruger NJ. The Bradford method for protein quantitation. The protein protocols handbook, 2009; 17-24. [CrossRef] google scholar
  • 26. Hilliard LR, Zhao X, Tan W. Immobilization of oligonucleotides onto silica nanoparticles for DNA hybridization studies. Anal Chim Acta, 2002; 470(1): 51-6. [CrossRef] google scholar
  • 27. Betancor L, Fuentes M, Dellamora-Ortiz G, Lopez-Gallego F, Hidal-go A, Alonso-Morales N, et al. Dextran aldehyde coating of glu-cose oxidase immobilized on magnetic nanoparticles prevents its inactivation by gas bubbles. J Mol Catal B: Enzym 2005; 32(3): 97-101. [CrossRef] google scholar
Yıl 2022, Cilt: 12 Sayı: 1, 18 - 23, 18.03.2022
https://doi.org/10.26650/experimed.2022.1058018

Öz

Proje Numarası

-

Kaynakça

  • 1. Andaç M, Denizli A. Affinity-recognition-based polymeric cryogels for protein depletion studies. Rsc Advances 2014; 4(59): 31130-41. [CrossRef] google scholar
  • 2. Bellei E, Bergamini S, Monari E, Fantoni LI, Cuoghi A, Ozben T, et al. High-abundance proteins depletion for serum proteomic anal-ysis: concomitant removal of non-targeted proteins. Amino Acids 2011; 40(1): 145-56. [CrossRef] google scholar
  • 3. Göktürk I, Tamahkar E, Yılmaz F, Denizli A. Protein depletion with bacterial cellulose nanofibers. J Chromatogr B 2018; 1099: 1-9. [CrossRef] google scholar
  • 4. Borberg E, Pashko S, Koren V, Burstein L, Patolsky F. Depletion of Highly Abundant Protein Species from Biosamples by the Use of a Branched Silicon Nanopillar On-Chip Platform. Anal Chem 2021; 93(43): 14527-36. [CrossRef] google scholar
  • 5. Siegmund R, Kiehntopf M, Deufel T. (2009). Evaluation of two dif-ferent albumin depletion strategies for improved analysis of hu-man CSF by SELDI-TOF-MS. Clin Biochem 2009; 42(10-11): 113643. [CrossRef] google scholar
  • 6. da Costa JP, Santos PS, Vitorino R, Rocha-Santos T, Duarte AC. How google scholar
  • low can you go? A current perspective on low-abundance pro-teomics. TrAC Trends Anal Chem 2017; 93: 171-82. [CrossRef] google scholar
  • 7. Uygun DA, Akduman B, Uygun M, Akgöl S, Denizli A. Purification of papain using reactive green 5 attached supermacroporous monolithic cryogel. Appl Biochem Biotechnol 2012; 167(3): 55263. [CrossRef] google scholar
  • 8. Akduman B, Uygun M, Çoban EP, Uygun DA, Bıyık H, Akgöl S. Reversible immobilization of urease by using bacterial cellulose nanofibers. Appl Biochem Biotechnol 2013; 171(8): 2285-94. [CrossRef] google scholar
  • 9. Uygun M, Uygun DA, Altunbaş C, Akgöl S, Denizli A. Dye attached nanoparticles for lysozyme adsorption. Sep Sci Technol 2014; 49(8): 1270-8. [CrossRef] google scholar
  • 10. Poddar S, Sharmeen S, Hage DS. Affinity monolith chromatog-raphy: a review of general principles and recent developments. Electrophoresis 2021; 42(24): 2577-98. [CrossRef] google scholar
  • 11. Andac M, Galaev I, Denizli A. Dye attached poly (hydroxyethyl methacrylate) cryogel for albumin depletion from human serum. J Sep Sci 2012; 35(9): 1173-82. [CrossRef] google scholar
  • 12. Wongchuphan R, Tey BT, Tan WS, Taip FS, Kamal SMM, Ling TC. Ap-plication of dye-ligands affinity adsorbent in capturing of rabbit immunoglobulin G. Biochem Eng J 2009; 45(3): 232-8. [CrossRef] google scholar
  • 13. Öztürk N, Tabak A, Akgöl S, Denizli A. Newly synthesized ben-tonite-histidine (Bent-His) micro-composite affinity sorbents for IgG adsorption. Colloids Surf A. Physicochem and Eng Asp 2007; 301(1-3): 490-7. [CrossRef] google scholar
  • 14. Alpay P, Uygun DA. Usage of immobilized papain for enzymatic hydrolysis of proteins. J Mol Catal B: Enzymatic 2015; 111: 56-63. [CrossRef] google scholar
  • 15. Akgöl S, Öztürk N, Denizli A. New generation polymeric nano-spheres for lysozyme adsorption. J Aappl PolymSci 2010;115(3): 1608-15. [CrossRef] google scholar
  • 16. Türkmen D, Öztürk N, Akgöl S, Elkak A, Denizli A. Phenylalanine containing hydrophobic nanospheres for antibody purification. Biotechnol Prog 2008; 24(6): 1297-1303. [CrossRef] google scholar
  • 17. Liao MH, Chen DH. Fast and efficient adsorption/desorption of protein by a novel magnetic nano-adsorbent. Biotechnol Lett 2002; 24(22): 1913-7. [CrossRef] google scholar
  • 18. Hegedûs I, Nagy E. Improvement of chymotrypsin enzyme sta-bility as single enzyme nanoparticles. Chem Eng Sci 2009; 64(5): 1053-60. [CrossRef] google scholar
  • 19. Uygun DA, Çorman ME, Öztürk N, Akgöl S, Denizli A. Poly (hy-droxyethyl methacrylate-co-methacryloylamidotryptophane) nanospheres and their utilization as affinity adsorbents for por-cine pancreas lipase adsorption. Mater Sci and Eng: C 2010; 30(8): 1285-90. [CrossRef] google scholar
  • 20. Yavuz H, Denizli A. Dye affinity hollow fibers for albumin purifica-tion. Macromol Biosci 2004; 4(2): 84-91. [CrossRef] google scholar
  • 21. Altıntaş EB, Denizli A. Efficient removal of albumin from human serum by monosize dye-affinity beads. J Chromatog B 2006; 832(2): 216-23. [CrossRef] google scholar
  • 22. Zhang J, Zhang Z, Song Y, Cai H. Bovine serum albumin (BSA) adsorption with Cibacron Blue F3GA attached chitosan micro-spheres. React Funct Polym 2006; 66(9): 916-23. [CrossRef] google scholar
  • 23. Tuzmen N, Kalburcu T, Uygun DA, Akgol S, Denizli A. A novel af-finity disks for bovine serum albumin purification. Appl Biochem Biotechnol 2015; 175(1): 454-68. [CrossRef] google scholar
  • 24. Orhan H, Evli S, Dabanca MB, Başbülbül G, Uygun M, Uygun DA. Bacteria killer enzyme attached magnetic nanoparticles. Mater Sci Eng C Mater Biol Appl 2019; 94: 558-64. [CrossRef] google scholar
  • 25. Kruger NJ. The Bradford method for protein quantitation. The protein protocols handbook, 2009; 17-24. [CrossRef] google scholar
  • 26. Hilliard LR, Zhao X, Tan W. Immobilization of oligonucleotides onto silica nanoparticles for DNA hybridization studies. Anal Chim Acta, 2002; 470(1): 51-6. [CrossRef] google scholar
  • 27. Betancor L, Fuentes M, Dellamora-Ortiz G, Lopez-Gallego F, Hidal-go A, Alonso-Morales N, et al. Dextran aldehyde coating of glu-cose oxidase immobilized on magnetic nanoparticles prevents its inactivation by gas bubbles. J Mol Catal B: Enzym 2005; 32(3): 97-101. [CrossRef] google scholar
Toplam 28 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Klinik Tıp Bilimleri
Bölüm Araştırma Makalesi
Yazarlar

Kevser Kuşat 0000-0003-4700-7835

Proje Numarası -
Yayımlanma Tarihi 18 Mart 2022
Gönderilme Tarihi 14 Ocak 2022
Yayımlandığı Sayı Yıl 2022 Cilt: 12 Sayı: 1

Kaynak Göster

Vancouver Kuşat K. Dye Ligand Affinity Nanoparticles for the Depletion of Biomolecules in Proteomics. Experimed. 2022;12(1):18-23.