Evaluation of Phytochemical Contents and Biological Activities of Salvia officinalis and Salvia triloba Grown with Organic Farming

Yıl 2023, Cilt: 48 Sayı: 1, 125 - 138, 01.03.2023

Burçin Özüpek , Sultan Pekacar , Didem Deliorman Orhan

https://doi.org/10.55262/fabadeczacilik.1175781

Cited By: 1

Öz

Salvia officinalis L., known as medicinal sage, and Salvia triloba L., known as Anatolian sage, belong to the Lamiaceae family and are species that usually grow in the Mediterranean region. In this study, it was aimed to evaluate the in vitro antidiabetic, antiobesity and antioxidant potentials of the extracts prepared by infusion technique from S. officinalis and S. triloba grown by organic farming methods. In addition, the effects of the extracts on the pancreatic cholesterol esterase enzyme were also investigated. Reverse Phase-HPLC technique was used to analyze the phytochemical contents of the extracts. At a concentration of 2 mg/mL, S. officinalis inhibited 64.69% ± 0.23, S. triloba 47.78 ± 2.11% on the α-glucosidase enzyme. Only S. triloba had an inhibitory effect on α-amylase and pancreatic lipase enzyme. On the pancreatic cholesterol esterase enzyme, inhibition values of S. triloba extract at all tested concentrations were found to be higher than S. officinalis extract. When the antioxidant potentials of the extracts were evaluated, the reducing power absorbance values were found to be the highest of the S. officinalis extract. The metal chelating capacity of both extracts at a concentration of 2 mg/mL was calculated as 100%. It was concluded that the ABTS radical scavenging activity of the extracts increased in a dose-dependent manner. With the Reverse Phase-YPSK technique, rosmarinic acid and hesperidin were found to be higher in S. officinalis extract. The presence of hesperidin in S. triloba was detected for the first time in this study. Considering all these findings, it was concluded that activity-guided isolation and in vivo activity studies should be performed because these two species grown by organic farming method have strong α-glucosidase enzyme inhibitory and antioxidant effects.

Anahtar Kelimeler

antioxidant, enzyme inhibition, phytochemistry, Reverse phase-HPLC, Salvia officinalis, Salvia triloba

Proje Numarası

TÜBİTAK 4004

Kaynakça

• Abubakari, A. R., & Bhopal, R. S. (2008). Systematic review on the prevalence of diabetes, overweight/obesity and physical inactivity in Ghanaians and Nigerians. Public health, 122(2), 173–182. https://doi.org/10.1016/j.puhe.2007.06.012
• Adımcılar, V., Kalaycıoğlu, Z., Aydoğdu, N., Dirmenci, T., Kahraman, A., & Erim, F. B. (2019). Rosmarinic and carnosic acid contents and correlated antioxidant and antidiabetic activities of 14 Salvia species from Anatolia. Journal of pharmaceutical and biomedical analysis, 175, 112763. https://doi.org/10.1016/j.jpba.2019.07.011
• American Diabetes Association (2005). Diagnosis and classification of diabetes mellitus. Diabetes care, 28 Suppl 1, S37–S42. https://doi.org/10.2337/diacare.28.suppl_1.s37
• Arıduru, R., & Arabacı, G. (2013). Ciğertaze otu (Salvia officinalis) bitkisinin antioksidan aktivitesinin belirlenmesi. Sakarya University Journal of Science, 17(2), 241-246.
• Asmat, U., Abad, K., & Ismail, K. (2016). Diabetes mellitus and oxidative stress-A concise review. Saudi pharmaceutical journal, 24(5), 547–553. https://doi.org/10.1016/j.jsps.2015.03.013
• Başyiğit, M., & Baydar, H. (2017). Tıbbi adaçayı (Salvia officinalis L.)’nda farklı hasat zamanlarının uçucu yağ ve fenolik bileşikler ile antioksidan aktivite üzerine etkisi. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 21(1), 131-137. DOI: 10.19113/sdufbed.55736
• Boukhary, R., Raafat, K., Ghoneim, A. I., Aboul-Ela, M., & El-Lakany, A. (2016). Anti-inflammatory and antioxidant activities of Salvia fruticosa: An HPLC determination of phenolic contents. Evidence-Based Complementary and Alternative Medicine, 2016. https://doi.org/10.1155/2016/7178105.
• Dincer, C., Topuz, A., Sahin-Nadeem, H., Ozdemir, K. S., Cam, I. B., Tontul, I., ... & Ay, S. T. (2012). A comparative study on phenolic composition, antioxidant activity and essential oil content of wild and cultivated sage (Salvia fruticosa Miller) as influenced by storage. Industrial Crops and Products, 39, 170-176. https://doi.org/10.1016/j.indcrop.2012.02.032
• Dinis, T. C., Maderia, V. M., & Almeida, L. M. (1994). Action of phenolic derivatives (acetaminophen, salicylate, and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Archives of biochemistry and biophysics, 315(1), 161–169. https://doi.org/10.1006/abbi.1994.1485
• Eidi A., & Eidi, M. (2009). Antidiabetic effects of sage (Salvia officinalis L.) leaves in normal and streptozotocin-induced diabetic rats. Diabetes & Metabolic Syndrome: Clinical Research & Reviews, 3(1), 40-44. https://doi.org/10.1016/j.dsx.2008.10.007
• Ercan, P., & El, S. N. (2018). Bioaccessibility and inhibitory effects on digestive enzymes of carnosic acid in sage and rosemary. International journal of biological macromolecules, 115, 933-939. https://doi.org/10.1016/j.ijbiomac.2018.04.139
• Gök, H. N., Deliorman Orhan, D., Gürbüz, İ., & Aslan, M. (2020). Activity‐guided isolation of α‐amylase, α‐glucosidase, and pancreatic lipase inhibitory compounds from Rhus coriaria L. Journal of Food Science, 85(10), 3220-3228. https://doi.org/10.1111/1750-3841.15438
• Hamrouni-Sellami, I., Rahali, F. Z., Rebey, I. B., Bourgou, S., Limam, F., & Marzouk, B. (2013). Total phenolics, flavonoids, and antioxidant activity of sage (Salvia officinalis L.) plants as affected by different drying methods. Food and Bioprocess Technology, 6(3), 806-817. https://doi.org/10.1007/s11947-012-0877-7
• Hussain, A., Hydrie, M. Z. I., Claussen, B., & Asghar, S. (2010). Type 2 Diabetes and obesity: A review. Journal of Diabetology, 2(1), 1-7.
• Jamous, R. M., Abu-Zaitoun, S. Y., Akkawi, R. J., & Ali-Shtayeh, M. S. (2018). Antiobesity and antioxidant potentials of selected palestinian medicinal plants. Evidence-Based Complementary and Alternative Medicine, 2018. https://doi.org/10.1155/2018/8426752
• Jung, H. A., Jin, S. E., Choi, R. J., Manh, H. T., Kim, Y. S., Min, B. S., … Choi, J. S. (2011). Anti-tumorigenic activity of sophoflavescenol against Lewis lung carcinoma in vitro and in vivo. Archives of pharmacal research, 34(12), 2087–2099. https://doi.org/10.1007/s12272-011-1212-y
• Kamatou, G. P., Makunga, N. P., Ramogola, W. P., & Viljoen, A. M. (2008). South African Salvia species: a review of biological activities and phytochemistry. Journal of ethnopharmacology, 119(3), 664–672. https://doi.org/10.1016/j.jep.2008.06.030
• Karık, Ü., & Sağlam, A. C. (2018). Marmara Bölgesi’ndeki Anadolu Adaçayı (Salvia fruticosa Mill.) populasyonlarının uçucu yağ bileşenleri, toplam antioksidan aktivite, toplam fenolik ve flavonoid madde miktarlarının belirlenmesi. Anadolu Ege Tarımsal Araştırma Enstitüsü Dergisi, 28(2), 37-47.
• Kosalec, I., Bakmaz, M., Pepeljnjak, S., & Vladimir-Knezević, S. (2004). Quantitative analysis of the flavonoids in raw propolis from northern Croatia. Acta pharmaceutica, 54(1), 65–72.
• Lee, Y. M., Kim, Y. S., Lee, Y., Kim, J., Sun, H., Kim, J. H., & Kim, J. S. (2012). Inhibitory activities of pancreatic lipase and phosphodiesterase from Korean medicinal plant extracts. Phytotherapy research, 26(5), 778–782. https://doi.org/10.1002/ptr.3644
• Lu, Y., & Foo, L. Y. (2002). Polyphenolics of Salvia--a review. Phytochemistry, 59(2), 117–140. https://doi.org/10.1016/s0031-9422(01)00415-0
• Mervić, M., Bival Štefan, M., Kindl, M., Blažeković, B., Marijan, M., & Vladimir-Knežević, S. (2022). Comparative Antioxidant, Anti-Acetylcholinesterase and Anti-α-Glucosidase Activities of Mediterranean Salvia Species. Plants, 11(5), 625. https://doi.org/10.3390/plants11050625
• Miliauskas, G., Venskutonis, P.R, Van Beek, T.A. (2004) Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chemistry, 85(2), 231-237. https://doi.org/10.1016/j.foodchem.2003.05.007
• Ngamukote, S., Mäkynen, K., Thilawech, T., & Adisakwattana, S. (2011). Cholesterol-lowering activity of the major polyphenols in grape seed. Molecules, 16(6), 5054–5061. https://doi.org/10.3390/molecules16065054
• Orhan, N., Deliorman Orhan, D., Gökbulut, A., Aslan, M., & Ergun, F. (2017). Comparative Analysis of Chemical Profile, Antioxidant, In-vitro and In-vivo Antidiabetic Activities of Juniperus foetidissima Willd. and Juniperus sabina L. Iranian journal of pharmaceutical research, 16(Suppl), 64–74.
• Pereira, O. R., Catarino, M. D., Afonso, A. F., Silva, A., & Cardoso, S. M. (2018). Salvia elegans, Salvia greggii and Salvia officinalis Decoctions: Antioxidant Activities and Inhibition of Carbohydrate and Lipid Metabolic Enzymes. Molecules, 23(12), 3169. https://doi.org/10.3390/molecules23123169
• Pizzale, L., Bortolomeazzi, R., Vichi, S., Überegger, E., & Conte, L. S. (2002). Antioxidant activity of sage (Salvia officinalis and S fruticosa) and oregano (Origanum onites and O indercedens) extracts related to their phenolic compound content. Journal of the Science of Food and Agriculture, 82(14), 1645-1651. https://doi.org/10.1002/jsfa.1240
• Sharma, Y., Fagan, J. B., & Schaefer, J. (2019). Ethnobotany, phytochemistry, cultivation and medicinal properties of Garden sage (Salvia officinalis L.) Journal of Pharmacognosy and Phytochemistry, 8(3), 3139-3148.
• Xu, J., Wei, K., Zhang, G., Lei, L., Yang, D., Wang, W., … Li, M. (2018). Ethnopharmacology, phytochemistry, and pharmacology of Chinese Salvia species: A review. Journal of ethnopharmacology, 225, 18–30. https://doi.org/10.1016/j.jep.2018.06.029
• Yildirim, A., Mavi, A., Oktay, M., Kara, A. A., Algur, O. F., & Bilaloglu, V. (2000). Comparison of antioxidant and antimicrobial activities of tilia (Tilia argentea Desf ex DC), sage (Salvia triloba l.), and black tea (Camellia sinensis) extracts. Journal of agricultural and food chemistry, 48(10), 5030–5034. https://doi.org/10.1021/jf000590k
• Zongo, C., Savadogo, A., Ouattara, L., Bassole, I. H. N., Ouattara, C. A. T., Ouattara, A. S., ... Traore, A. S. (2010). Polyphenols content, antioxidant and antimicrobial activities of Ampelocissus grantii (Baker) Planch.(Vitaceae): a medicinal plant from Burkina Faso. IJP-International Journal of Pharmacology, 6(6), 880-887. https://doi.org/10.3923/ijp.2010.880.887