DNA Binding, Nuclease/Photonuclease, and Phototoxicty Properties of Water Soluble Silicon (IV) Phthalocyanine

Yıl 2024, Cilt: 3 Sayı: 4, 126 - 133

Gökçe Seyhan , Ceren Boguslu , Can Özgür Yalçın , Zekeriya Bıyıklıoğlu , Burak Barut

https://doi.org/10.59518/farabimedj.1579677

Öz

Photodynamic therapy (PDT) is known as a method in which photosensitizers produce reactive oxygen species in the presence of light and oxygen, leading to cell death. In this paper, DNA interaction properties of bis[4-({8)-[3-(trimethylamino)phenoxy]octyl}oxy)] substituted silicon (IV) phthalocyanine (GsB-SiPc) were examined using a UV-Vis spectrophometer and agarose gel electrophoresis techniques. Afterwards, cytotoxic/phototoxic effects of GsB-SiPc were examined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays on A549 cells. The results showed that GsB-SiPc bound to ct-DNA via a groove binding mode. In nuclease/photonuclease experiments, GsB-SiPc had low nuclease activity in the dark but it showed high photonuclease activity in the presence of light, depending on compound concentration and light dose. In addition, GsB-SiPc demonstrated remarkable phototoxicity toward human lung adenocarcinoma (A549) cell line at 50 and 100 µM in the presence of light. The in vitro data revealed the potential of GsB-SiPc as a photodynamic therapy agent for the treatment of lung cancer. These findings need to be supported by further studies.

Anahtar Kelimeler

DNA Binding, DNA Nuclease, Lung Cancer, Photodynamic Therapy, Silicon (IV) Phthalocyanine

Etik Beyan

Since we did not use human/animal or human/animal data in this study, our study does not require ethics committee approval.

Suda Çözünür Silisyum (IV) Ftalosiyaninin DNA Bağlanma, Nükleaz/Fotonükleaz ve Fototoksik Özellikleri

Öz

Fotodinamik terapi (PDT), fotosensitizörlerin ışık ve oksijen varlığında reaktif oksijen türleri ürettiği ve hücre ölümüne yol açtığı bilinen bir yöntemdir. Bu makalede, bis[4-({8)-[3-(trimetilamino)fenoksi]oktil}oksi)] yan grubu içeren silisyum (IV) ftalosiyaninin (GsB-SiPc) DNA etkileşim özellikleri bir UV-Vis spektrofotometresi ve agaroz jel elektroforezi teknikleri kullanılarak incelenmiştir. Daha sonra, GsB-SiPc'nin sitotoksik/fototoksik etkileri A549 hücreleri üzerinde 3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolium bromür (MTT) deneyleri kullanılarak incelenmiştir. Sonuçlar, GsB-SiPc'nin ct-DNA'ya bir oluk bağlama modu aracılığıyla bağlandığını göstermiştir. Nükleaz/fotonükleaz deneylerinde, GsB-SiPc karanlıkta düşük nükleaz aktivitesine sahipti ancak bileşik konsantrasyonuna ve ışık dozuna bağlı olarak ışık varlığında yüksek fotonükleaz aktivitesi gösterdi. Ek olarak, GsB-SiPc ışık varlığında 50 ve 100 µM'de insan akciğer adenokarsinomu (A549) hücre hattına karşı dikkate değer fototoksisite gösterdi. İn vitro veriler, GsB-SiPc'nin akciğer kanserinin tedavisi için bir fotodinamik terapi ajanı olarak potansiyelini ortaya koydu. Bu bulguların daha fazla çalışmayla desteklenmesi gerekiyor.

Anahtar Kelimeler

Akciğer Kanseri, DNA Bağlama, DNA Nükleaz, Fotodinamik Terapi, Silisyum (IV) Ftalosiyanin

Kaynakça

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