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Citric acid production using rotating biodisc reactor (RBR)

Yıl 2022, , 25 - 29, 30.04.2022
https://doi.org/10.51753/flsrt.1035228

Öz

Citric acid is one of the most remarkable industrial goods fabricated by industrial fermentation using filamentous fungus. When Aspergillus niger, a filamentous fungus, is produced under suitable conditions, high amounts of citric acid are obtained. This study aims to explain the citric acid synthesis studied in a biodisc reactor using A. niger. Various physiological parameters have been investigated in order to determine the optimum citric acid synthesis in biodisc reactor. Optimum incubation time was found to be as 168 hours in the study. The optimum value of the incubation temperature was determined to be 30°C. The optimum value of the initial pH was found to be 3.8. Optimum citric acid synthesis occurred when the disc rotation speed was 2 rpm. In addition, the optimum value of the initial sugar (sucrose) concentration was determined to be 20%. In a semi-continuous production study with the renewal of the medium after a certain incubation period, it was determined that the citric acid yield increased approximately 3 times compared to the batch system. As a result, the highest value of citric acid was determined as 67.65 g/L.

Kaynakça

  • Akpinar, O., Ucar, B. F., & Yalcin, H. T. (2011). Screening and regulation of alkaline extracellular protease and ribonuclease production of Yarrowia lipolytica strains isolated and identified from different cheeses in Turkey. Annals of Microbiology, 61(4), 907-915.
  • Amenaghawon, N. A., Areguamen, S. O., Agbroko, N. T., Ogbeide, S. E., & Okieimen, C. O. (2013). Modelling and statistical optimisation of acid production from solid state fermentation of sugar cane bagasse using Aspergillus niger. International Journal of Sciences, 2, 56-62.
  • Anastassiadis, S., Aivaidis, A., & Wandrey C. (2002). Citric acid production by Candida strains under intracellular nitrogen limitation. Applied Microbiology and Biotechnology, 60, 81-87.
  • Apelblat, A. (2016). Representation of volumetric and compressibility properties in binary and ternary system. Journal of Solution Chemistry, 45, 1530-1547.
  • Arzumanov, T. E., Shishkanova, N. V., & Finogenova, T. V. (2000). Biosynthesis of citric acid by Yarrowia lipolytica repeat-batch culture on ethanol. Applied Microbiology and Biotechnology, 53(5), 525-529.
  • Currie, J. N. (1917). The citric acid fermentation of Aspergillus niger. Journal of Biological Chemistry, 31,15-37.
  • Corbaci, C. (2008). The effect of sodium on the growth of Debaryomyces hansenii strains at high temperature and extreme pH, Master Thesis, (pp.1-186). Ege University, Institute of Science, Turkey.
  • Demirel, G. (2003). Citric acid production using immobilized Aspergillus niger and investigation of various effects on citric acid production, Master Thesis, (pp. 1-56). Gazi University Institute of Science and Technology, Turkey.
  • Dhillon, G. S., Lea Rosine, G. M., Kaur, S., Hedge, K., Brar, S. K., Drogui, P., & Verma, M. (2017). Novel biomaterials from citric acid fermentation as biosorbents for removal of metals from waste chromated copper arsenate wood leachate. International Biodeterioration and Biodegradation, 119,147-154.
  • FAO/WHO (2002) Food and Agriculture Organization of the United Nations and World Health Organization. Human Vitamin and Mineral Requirements, Rome. https://www.fao.org/3/y2809e/y2809e.pdf, Last Accessed on March 13, 2022.
  • Gonçalves, C., Lopes, M., Ferreira, P. J., & Belo, I. (2009). Biological treatment of olive mill wastewater by non-conventional yeasts. Bioresource Technology, 100, 3759-3763.
  • Guven, M. (1995). Citric acid production using Aspergillus niger (Fürsan) in Biodisc Reactor, Doctoral Dissertation, (pp.1-85). Hacettepe University, Institute of Science and Technology, Turkey.
  • Kamzolova, S. V., Lunina, J. N., & Morgunov, I. G. (2011). Biochemistry of citric acid production from rapeseed oil by Yarrowia lipolytica yeast,.Journal of American Oil Chemistry Society, 88, 1965-1976.
  • Kareem, S. O., Akpan, I., & Alebiowu, O. O. (2010). Production of citric acid by Aspergillus niger using pineapple waste. Malaysian Journal of Microbiology, 6(2), 161-165.
  • Kargi, F., & Eker, S. (2002). Comparison of performances of rotating perforated tubes and rotating biodiscs biofilm reactors for wastewater treatment. Process Biochemistry, 37, 1201-1206.
  • Kudzai, C. T., Ajay, K. & Ambika, P. (2016). Citric acid production by Aspergillus niger using different substrates. Malaysian Journal of Microbiology, 12(3), 199-204.
  • Lotfy, W. A., Ghanem, K. M., & El-Helow, E. R. (2007). Citric acid production by a novel Aspergillus niger isolate: II. Optimization of process parameters through statistical experimental designs. Bioresource Technology, 98, 3470-3477.
  • Marier, J. R., & Boulet M. (1958). Direct determination of citric acid in milk with an improved pyridine-acetic anhydride method. Jornal of Dairy Science, 4, 1683-1692.
  • Mattey, M. (1992). The production of organic acids. Critical Reviews Biotechnology, 12(1/2), 87-132.
  • Max, B., Salgado, J. M., Rodriguez, N., Cortes, S., Converti, A., & Dominguez, J. M. (2010). Biotechnological production of citric acid. Brazilian Journal of Microbiology, 41, 862-875.
  • Moeller, L., Strehlitz, B., Aurıch, A., Zehnsdorf, A. & Bley, T., (2007). Optimization of citric acid production from glucose by Yarrowia lipolytica. Engineering in Life Sciences, 7(5), 504-511.
  • Morgunov, I., Kamzolova, S., & Lunina, J. (2013). The citric acid production from raw glycerol by Yarrowia lipolytica yeast and its regulation. Applied Microbiology Biotechnology, 97, 7387-7397.
  • Padvi, M. A., & Pawar, S. V. (2011). Factors affecting production of citric acid. International Referred Research Journal, 3, 37-38.
  • Peksel, A., Tokatman, K. A., & Arısan Ataç, İ. (2003). Investigation of the effects of some polyols on yield during citric acid fermentation by Aspergıllus niger. Journal of Yıldız Technical University, 4, 1-6.
  • Pessoa, D. F., Dıasde, C., & Angela, C. (1982). Production of citric acid by Aspergillus niger. Revista de Microbiologia, 13(2), 225-229.
  • Prasad, M. P. D., Sridevi, V., Surendra N. V., Reddy, O. V. S., & Harsha, N. (2013). Studies on fermentative production of citric acid by Aspergillus niger isolate using sorghum malt and it’s optimization. International Journal of Innovative Research in Science, Engineering and Technology, 2, 2961-2968.
  • Rajoka, M. I., Ahmad, M. N., Shahıd, R., Latıf, F., & Parvez, S. (1998). Citric acid production from sugar-cane molasses by cultures of Aspergillus niger. Biologia, 44(1), 241-253.
  • Ramesh, T., & Kalaiselvam, M. (2011). An experimental study on citric acid production by Aspergillus niger using Gelidiella acerosa as a substrate. Indian Journal of Microbiology, 51(3), 289-293.
  • Sahasrabudhe, N. A., & Sankpal, N. V. (2001). Production of organic acids and metabolites of fungi for food industry. In: Arora D. K., Berka R. M., Singh (eds) Applied Mycology and Biotechnology (pp. 387-425). Elsevier Amsterdam.
  • Sidal, U., & Taskin-Ozkale, E. (2003). Rhamnolipid production from olive oil mill effluent (OOME) using the rotating biodisc reactor. Biologia, Bratislava, 58(6),1083-1088.
  • Soccol, C.R., Vandenberghe, L. P. S., Rodrigues, C., & Pandey, A. (2006). New perspectives for citric acid production and application, Food Technology and Biotechnology, 44(2), 141-149.
  • Souza, K., Schwan, R., & Dias, D. (2014). Lipid and citric acid production by wild yeasts grown in glycerol. Journal of Microbiology and Biotechnology, 24, 497-506.
  • Vergano, M. G., Femandez, N., Sorıa, M. A., & Kerber, M. S. (1996). Influence of inoculum preparation on citric acid preparation by Aspergillus niger. Journal of Biotechnology, 12(6), 655-656.
  • Verhoff, F. H. (2003). Citric acid. In: Elvers B., Hawkins S., Harrer R., Pikart-Müller M. (eds) Ullmann’s Encyclopedia of Industrial Chemistry (pp. 8:525-531). Germany.
  • Yalcin, S. K., Bozdemir, M. T., & Ozbas, Z. Y. (2010). Citric acid production by yeasts: Fermentation conditions, process optimization and strain improvement, Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology, 9, 1374-1382.
  • Wieczorek, S., & Brauer, H. (1998). Continuous production of citric acid with recirculation of the fermentation broth after product recovery. Bioprocess Engineering, 18(1), 1-6.
  • Wyrzykowski, D., Hebanowska, E., Nowak-Wiczk, G., Makowski, M., & Chmurzyński, L. (2011). Thermal behaviour of citric acid and isomeric aconitic acids. Journal of Thermal Analysis and Calorimetry, 104(2), 731-735.
Yıl 2022, , 25 - 29, 30.04.2022
https://doi.org/10.51753/flsrt.1035228

Öz

Kaynakça

  • Akpinar, O., Ucar, B. F., & Yalcin, H. T. (2011). Screening and regulation of alkaline extracellular protease and ribonuclease production of Yarrowia lipolytica strains isolated and identified from different cheeses in Turkey. Annals of Microbiology, 61(4), 907-915.
  • Amenaghawon, N. A., Areguamen, S. O., Agbroko, N. T., Ogbeide, S. E., & Okieimen, C. O. (2013). Modelling and statistical optimisation of acid production from solid state fermentation of sugar cane bagasse using Aspergillus niger. International Journal of Sciences, 2, 56-62.
  • Anastassiadis, S., Aivaidis, A., & Wandrey C. (2002). Citric acid production by Candida strains under intracellular nitrogen limitation. Applied Microbiology and Biotechnology, 60, 81-87.
  • Apelblat, A. (2016). Representation of volumetric and compressibility properties in binary and ternary system. Journal of Solution Chemistry, 45, 1530-1547.
  • Arzumanov, T. E., Shishkanova, N. V., & Finogenova, T. V. (2000). Biosynthesis of citric acid by Yarrowia lipolytica repeat-batch culture on ethanol. Applied Microbiology and Biotechnology, 53(5), 525-529.
  • Currie, J. N. (1917). The citric acid fermentation of Aspergillus niger. Journal of Biological Chemistry, 31,15-37.
  • Corbaci, C. (2008). The effect of sodium on the growth of Debaryomyces hansenii strains at high temperature and extreme pH, Master Thesis, (pp.1-186). Ege University, Institute of Science, Turkey.
  • Demirel, G. (2003). Citric acid production using immobilized Aspergillus niger and investigation of various effects on citric acid production, Master Thesis, (pp. 1-56). Gazi University Institute of Science and Technology, Turkey.
  • Dhillon, G. S., Lea Rosine, G. M., Kaur, S., Hedge, K., Brar, S. K., Drogui, P., & Verma, M. (2017). Novel biomaterials from citric acid fermentation as biosorbents for removal of metals from waste chromated copper arsenate wood leachate. International Biodeterioration and Biodegradation, 119,147-154.
  • FAO/WHO (2002) Food and Agriculture Organization of the United Nations and World Health Organization. Human Vitamin and Mineral Requirements, Rome. https://www.fao.org/3/y2809e/y2809e.pdf, Last Accessed on March 13, 2022.
  • Gonçalves, C., Lopes, M., Ferreira, P. J., & Belo, I. (2009). Biological treatment of olive mill wastewater by non-conventional yeasts. Bioresource Technology, 100, 3759-3763.
  • Guven, M. (1995). Citric acid production using Aspergillus niger (Fürsan) in Biodisc Reactor, Doctoral Dissertation, (pp.1-85). Hacettepe University, Institute of Science and Technology, Turkey.
  • Kamzolova, S. V., Lunina, J. N., & Morgunov, I. G. (2011). Biochemistry of citric acid production from rapeseed oil by Yarrowia lipolytica yeast,.Journal of American Oil Chemistry Society, 88, 1965-1976.
  • Kareem, S. O., Akpan, I., & Alebiowu, O. O. (2010). Production of citric acid by Aspergillus niger using pineapple waste. Malaysian Journal of Microbiology, 6(2), 161-165.
  • Kargi, F., & Eker, S. (2002). Comparison of performances of rotating perforated tubes and rotating biodiscs biofilm reactors for wastewater treatment. Process Biochemistry, 37, 1201-1206.
  • Kudzai, C. T., Ajay, K. & Ambika, P. (2016). Citric acid production by Aspergillus niger using different substrates. Malaysian Journal of Microbiology, 12(3), 199-204.
  • Lotfy, W. A., Ghanem, K. M., & El-Helow, E. R. (2007). Citric acid production by a novel Aspergillus niger isolate: II. Optimization of process parameters through statistical experimental designs. Bioresource Technology, 98, 3470-3477.
  • Marier, J. R., & Boulet M. (1958). Direct determination of citric acid in milk with an improved pyridine-acetic anhydride method. Jornal of Dairy Science, 4, 1683-1692.
  • Mattey, M. (1992). The production of organic acids. Critical Reviews Biotechnology, 12(1/2), 87-132.
  • Max, B., Salgado, J. M., Rodriguez, N., Cortes, S., Converti, A., & Dominguez, J. M. (2010). Biotechnological production of citric acid. Brazilian Journal of Microbiology, 41, 862-875.
  • Moeller, L., Strehlitz, B., Aurıch, A., Zehnsdorf, A. & Bley, T., (2007). Optimization of citric acid production from glucose by Yarrowia lipolytica. Engineering in Life Sciences, 7(5), 504-511.
  • Morgunov, I., Kamzolova, S., & Lunina, J. (2013). The citric acid production from raw glycerol by Yarrowia lipolytica yeast and its regulation. Applied Microbiology Biotechnology, 97, 7387-7397.
  • Padvi, M. A., & Pawar, S. V. (2011). Factors affecting production of citric acid. International Referred Research Journal, 3, 37-38.
  • Peksel, A., Tokatman, K. A., & Arısan Ataç, İ. (2003). Investigation of the effects of some polyols on yield during citric acid fermentation by Aspergıllus niger. Journal of Yıldız Technical University, 4, 1-6.
  • Pessoa, D. F., Dıasde, C., & Angela, C. (1982). Production of citric acid by Aspergillus niger. Revista de Microbiologia, 13(2), 225-229.
  • Prasad, M. P. D., Sridevi, V., Surendra N. V., Reddy, O. V. S., & Harsha, N. (2013). Studies on fermentative production of citric acid by Aspergillus niger isolate using sorghum malt and it’s optimization. International Journal of Innovative Research in Science, Engineering and Technology, 2, 2961-2968.
  • Rajoka, M. I., Ahmad, M. N., Shahıd, R., Latıf, F., & Parvez, S. (1998). Citric acid production from sugar-cane molasses by cultures of Aspergillus niger. Biologia, 44(1), 241-253.
  • Ramesh, T., & Kalaiselvam, M. (2011). An experimental study on citric acid production by Aspergillus niger using Gelidiella acerosa as a substrate. Indian Journal of Microbiology, 51(3), 289-293.
  • Sahasrabudhe, N. A., & Sankpal, N. V. (2001). Production of organic acids and metabolites of fungi for food industry. In: Arora D. K., Berka R. M., Singh (eds) Applied Mycology and Biotechnology (pp. 387-425). Elsevier Amsterdam.
  • Sidal, U., & Taskin-Ozkale, E. (2003). Rhamnolipid production from olive oil mill effluent (OOME) using the rotating biodisc reactor. Biologia, Bratislava, 58(6),1083-1088.
  • Soccol, C.R., Vandenberghe, L. P. S., Rodrigues, C., & Pandey, A. (2006). New perspectives for citric acid production and application, Food Technology and Biotechnology, 44(2), 141-149.
  • Souza, K., Schwan, R., & Dias, D. (2014). Lipid and citric acid production by wild yeasts grown in glycerol. Journal of Microbiology and Biotechnology, 24, 497-506.
  • Vergano, M. G., Femandez, N., Sorıa, M. A., & Kerber, M. S. (1996). Influence of inoculum preparation on citric acid preparation by Aspergillus niger. Journal of Biotechnology, 12(6), 655-656.
  • Verhoff, F. H. (2003). Citric acid. In: Elvers B., Hawkins S., Harrer R., Pikart-Müller M. (eds) Ullmann’s Encyclopedia of Industrial Chemistry (pp. 8:525-531). Germany.
  • Yalcin, S. K., Bozdemir, M. T., & Ozbas, Z. Y. (2010). Citric acid production by yeasts: Fermentation conditions, process optimization and strain improvement, Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology, 9, 1374-1382.
  • Wieczorek, S., & Brauer, H. (1998). Continuous production of citric acid with recirculation of the fermentation broth after product recovery. Bioprocess Engineering, 18(1), 1-6.
  • Wyrzykowski, D., Hebanowska, E., Nowak-Wiczk, G., Makowski, M., & Chmurzyński, L. (2011). Thermal behaviour of citric acid and isomeric aconitic acids. Journal of Thermal Analysis and Calorimetry, 104(2), 731-735.
Toplam 37 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Yapısal Biyoloji
Bölüm Araştırma Makaleleri
Yazarlar

Uğur Sıdal 0000-0002-1562-6239

Yayımlanma Tarihi 30 Nisan 2022
Gönderilme Tarihi 10 Aralık 2021
Yayımlandığı Sayı Yıl 2022

Kaynak Göster

APA Sıdal, U. (2022). Citric acid production using rotating biodisc reactor (RBR). Frontiers in Life Sciences and Related Technologies, 3(1), 25-29. https://doi.org/10.51753/flsrt.1035228

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Frontiers in Life Sciences and Related Technologies is licensed under a Creative Commons Attribution 4.0 International License.