Epidemiological analysis of the diagnostic results of COVID-19 infection in Bishkek by real-time PCR method

Abstract

The aim of the research is to analyze the diagnostic results of infected people with the COVID-19 in Bishkek. Therefore, in this study, with the aim of epidemiologically analyzing the COVID-19 infection in the city, diagnostic PCR research was carried out at the Center for Quarantine and Highly Dangerous Infections of the Ministry of Health of the Kyrgyz Republic in the Laboratory of Virology and Rare Infections. During the period of operation from 01st May 2020 to 31st March 2021, 28,863 samples from patients of the Sverdlovsky and Pervomaisky districts of Bishkek were examined by the real-time PCR. For laboratory diagnostics of COVID-19, according to the recommendations of the WHO and Russian regulatory documents, obtained biomaterials from the upper respiratory tract like swabs from the nasopharynx and oropharynx were used. Biological materials were collected from patients with symptoms of upper respiratory tract infections and from patients who contacted with COVID-19 infected people applied to Family Medicine Centers in Bishkek city for the purpose of preventive examination and treatment. The samples were identified by real-time PCR, in accordance with the “biological safety” rules. From 28,863 samples 3,255 positive and 25,575 negative results were found. In the study, the largest number of samples from patients with symptoms of COVID-19 infection was recorded in June, and July, 2021, as 7,604, and 4,074, respectively. In June, 738 positive results were detected from 7,604 samples, while in July, 540 positive results were detected from 4,074 samples. This period is estimated to be the acute dynamic period of the epidemiological situation in Bishkek (Pervomaisky and Sverdlovsky districts). The epidemiological situation was stable in August and September, with the number of infected in August was 17 cases, and in September 2 cases. However, since September 2020, the number of infected people with COVID-19 has been growing. Besides, the results of PCR diagnostics from 01st January to 31st March 2021 indicated that the epidemiological situation was assessed as “relatively stable”.

Keywords

• Amplification
• cDNA
• COVID-19
• RdRP
• RT-PCR
• SARS-CoV-2

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