Infuse herbal oils: a comparative study of wheat germ and tomato seed oils

Abstract

The use of traditional herbal medicine products has recently been revived, with special emphasis on the use of herbal oils in non-invasive wound treatment. Extraction conditions are very important in seed oils and especially suitable temperatures at which the extraction process is carried out. is vital for the preservation of primary and secondary metabolites. In this study, wheat germ (WG) was collected from the Central Anatolia Region and tomato seed (TS) was collected from the Aegean Region. Oils were obtained from these two seeds by cold pressing and their infused forms (WGO-TSO) were prepared. To investigate the in vitro activities of these study groups, cytotoxicity, wound healing, and antioxidant capacity tests were performed on HaCaT (Human keratinocyte) and Vero (Monkey kidney fibroblast-like) cell lines. According to the results of the intracellular cytotoxicity analysis, a common dose of 25 µg/mL was determined to be non-toxic for all groups, and this dose was then used as the working dose. Antioxidant capacity studies, in cells under oxidative stress with hydrogen peroxide, yielded positive results for all working groups and the WGO/TSO group showed statistically superior outcomes. In addition, similar results were obtained in wound healing experiments. As a result, using oils in the form of infusion may be more effective in combating oxidative stress and promoting wound healing than using oil alone.

Keywords

• Antioxidant capacity
• cell culture
• infuse oils
• wound healing

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