The genetic diversity and certain disease assessments of wild tomato genetic resources and integration into breeding

Yıl 2025, Sayı: Advanced Online Publication, 154 - 163

Hasan Can

https://doi.org/10.51753/flsrt.1711383

Öz

Changing climate and pandemic-level disease affect tomato production as with all agricultural products. Moreover, the emergence of new diseases such as tomato brown rugose fruit virus has increased in recent years, and the cultivars are insufficient in production. To breed new cultivars that can respond to today's demands, the characteristics of genetic resources, including wild forms, need to be determined. In this context, the study attempted to screen some important tomato diseases, such as Tomato Spotted Wilt Virus (TSWV), Tomato yellow leaf curl virus (TYLCV), and root-knot nematode at the molecular level in tomato wild collections, and to reveal the genetic relationship between the wild forms available. For genetic diversity, 7 InDel and 8 SRAP markers (combination of 8 markers) and their combinations were included in the study, and a total of 60 polymorphic bands were scored from the given primer sets. The genetic relationship between wild forms was determined to be within 0.40 according to the simple matching similarity coefficient, and the result was also supported by principal coordinate analysis. The linked loci were amplified in wild forms and scored as resistant and susceptible using relevant primer sets. By evaluating all of these, the research indicated that the PI 128643 (Peru), PI 127830 (Peru), PI 212409 (Venezuela), PI 246586(Peru), PI 479211 (Colombia), and PI 487625 (Costa Rica) accessions possess considerable potential to enhance the breeding program.

Anahtar Kelimeler

Breeding , diseases , genetic resources , molecular markers , wild tomatoes

Proje Numarası

yok

Kaynakça

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