Research Article

Microbiological Hygiene Assessment of a Commercial Bakery: From Raw Materials and Processing Environment to Final Products

Volume: 1 Number: 2 October 29, 2026

Microbiological Hygiene Assessment of a Commercial Bakery: From Raw Materials and Processing Environment to Final Products

Abstract

This study evaluated the microbiological quality of raw materials, final products, processing water, air, and production surfaces in a commercial bakery producing lavash and baklava phyllo. Sampling was conducted during three independent visits. Total viable counts (TVC), yeasts and molds, coliform bacteria, Escherichia coli, and presumptive Staphylococcus aureus were determined as appropriate, and water activity (aw) of the final products was measured. Airborne microbial counts varied among production areas and visits, with TVC ranging from 3 to 27 CFU/plate/15 min in the lavash line and from 3 to 34 CFU/plate/15 min in the baklava phyllo line. Among raw materials, whole-wheat flour showed the highest microbial loads, with TVC of 2.0 × 10⁴–4.3 × 10⁴ CFU/g and coliform counts of 4.3 to >110 MPN/g. In contrast, both lavash and baklava phyllo consistently showed TVC and yeast and mold counts below 10 CFU/g, while coliforms and E. coli were not detected. Microbial contamination of production surfaces varied considerably among sampling visits, with localized high counts observed particularly on dough-handling areas and the flour sack. Processing water showed low microbial loads, and coliforms and E. coli were not detected in any of the water samples. Water activity ranged from 0.878 to 0.885 in lavash and from 0.771 to 0.891 in baklava phyllo. Overall, the findings indicate satisfactory microbiological quality of the final products but also demonstrate the importance of routine environmental monitoring to identify localized and temporally variable contamination within bakery production environments.

Keywords

Bakery hygiene, Lavash, Baklava phyllo, Microbial contamination, Environmental monitoring, Food-contact surfaces

Supporting Institution

This study was supported by the Scientific Research Projects Coordination Unit of Ankara University under the Graduate Thesis Project scheme.

Project Number

Project No. 16L0443005

References

  1. G. N. Charão et al., “Level of compliance with good manufacturing practices and microbiological profile of mixed fresh sausages,” Ciência Animal Brasileira, vol. 25, Art. no. 76582E, 2024, doi: 10.1590/1809-6891v25e-76582E.
  2. A. S. Quintanilla-Martínez, L. Aguirre-Güitrón, L. D. Espinosa-Chaurand, M. Diaz-Ramírez, and A. De Jesús Cortés-Sánchez, “Microbiological analysis of the air in a popular fish processing and marketing area,” Applied Biosciences, vol. 1, no. 3, pp. 299–314, 2022, doi: 10.3390/applbiosci1030019.
  3. R. V. C. Cardoso et al., “Physicochemical characterization and microbiology of wheat and rye flours,” Food Chemistry, vol. 280, pp. 123–129, 2019, doi: 10.1016/j.foodchem.2018.12.063.
  4. F. Forghani, M. den Bakker, J. Liao, A. S. Payton, A. N. Futral, and F. Diez‐Gonzalez, “Salmonella and enterohemorrhagic Escherichia coli serogroups O45, O121, O145 in wheat flour: Effects of long-term storage and thermal treatments,” Frontiers in Microbiology, vol. 10, Art. no. 323, 2019, doi: 10.3389/fmicb.2019.00323.
  5. I. S. Hines et al., “Persistence comparison of two Shiga-toxin producing Escherichia coli (STEC) serovars during long-term storage and thermal inactivation in various wheat flours,” PLOS ONE, vol. 19, no. 3, Art. no. e0299922, 2024, doi: 10.1371/journal.pone.0299922.
  6. H. Zhang, E. Yamamoto, J. Murphy, C. Carrillo, K. Hardie, and A. Locas, “Microbiological survey of wheat flour sold at retail in Canada, 2018 to 2019,” Journal of Food Protection, vol. 84, no. 4, pp. 647–654, 2021, doi: 10.4315/JFP-20-297.
  7. G. B. Olukotun, Z. Labbo, O. G. Abakpa, B. B. Adamu, and P. N. Ajulor, “The mycotoxin risks lurking in breads sold in Abuja Metropolis,” Asian Food Science Journal, vol. 20, no. 7, pp. 47–56, 2021, doi: 10.9734/AFSJ/2021/v20i730320.
  8. Ş. Arda and A. Aydın, “Hammadde kalitesi ile bazı hijyen parametrelerinin yufkanın mikrobiyolojik kalitesi arasındaki ilişki üzerine bir araştırma,” İstanbul Üniversitesi Veteriner Fakültesi Dergisi, vol. 37, no. 2, pp. 135–147, 2011.
  9. E. Dümen, Ö. Çetin, and F. H. Sezgin, “Unlu mamul işletmelerinde temas yüzeylerinin ve aletlerin mikrobiyolojik kirliliğinin araştırılması,” Türk Mikrobiyoloji Cemiyeti Dergisi, vol. 39, no. 3–4, pp. 108–114, 2009.
  10. S. Dagnas, B. Onno, and J.-M. Membré, “Modeling growth of three bakery product spoilage molds as a function of water activity, temperature and pH,” International Journal of Food Microbiology, vol. 186, pp. 95–104, 2014, doi: 10.1016/j.ijfoodmicro.2014.06.022.
APA
Koçaklı, M. H., & Ayhan, K. (2026). Microbiological Hygiene Assessment of a Commercial Bakery: From Raw Materials and Processing Environment to Final Products. Food Science Insights, 1(2), 74-85. https://izlik.org/JA93FF77KK
AMA
1.Koçaklı MH, Ayhan K. Microbiological Hygiene Assessment of a Commercial Bakery: From Raw Materials and Processing Environment to Final Products. Food Science Insights. 2026;1(2):74-85. https://izlik.org/JA93FF77KK
Chicago
Koçaklı, Müjde Havva, and Kamuran Ayhan. 2026. “Microbiological Hygiene Assessment of a Commercial Bakery: From Raw Materials and Processing Environment to Final Products”. Food Science Insights 1 (2): 74-85. https://izlik.org/JA93FF77KK.
EndNote
Koçaklı MH, Ayhan K (October 1, 2026) Microbiological Hygiene Assessment of a Commercial Bakery: From Raw Materials and Processing Environment to Final Products. Food Science Insights 1 2 74–85.
IEEE
[1]M. H. Koçaklı and K. Ayhan, “Microbiological Hygiene Assessment of a Commercial Bakery: From Raw Materials and Processing Environment to Final Products”, Food Science Insights, vol. 1, no. 2, pp. 74–85, Oct. 2026, [Online]. Available: https://izlik.org/JA93FF77KK
ISNAD
Koçaklı, Müjde Havva - Ayhan, Kamuran. “Microbiological Hygiene Assessment of a Commercial Bakery: From Raw Materials and Processing Environment to Final Products”. Food Science Insights 1/2 (October 1, 2026): 74-85. https://izlik.org/JA93FF77KK.
JAMA
1.Koçaklı MH, Ayhan K. Microbiological Hygiene Assessment of a Commercial Bakery: From Raw Materials and Processing Environment to Final Products. Food Science Insights. 2026;1:74–85.
MLA
Koçaklı, Müjde Havva, and Kamuran Ayhan. “Microbiological Hygiene Assessment of a Commercial Bakery: From Raw Materials and Processing Environment to Final Products”. Food Science Insights, vol. 1, no. 2, Oct. 2026, pp. 74-85, https://izlik.org/JA93FF77KK.
Vancouver
1.Müjde Havva Koçaklı, Kamuran Ayhan. Microbiological Hygiene Assessment of a Commercial Bakery: From Raw Materials and Processing Environment to Final Products. Food Science Insights [Internet]. 2026 Oct. 1;1(2):74-85. Available from: https://izlik.org/JA93FF77KK