Myrtus communis (Mersin) ve Üzüm Çekirdeği Özütü Kullanılarak Gümüş Nanoparçacık Biyosentezi ve Karakterizasyonu

Abstract

Bitkisel malzemeler ve özütlerin, metal nanoparçacıkların biyosentezinde önemleri giderek artmaktadır. Biyosentez, nanoparçacık sentezinde hem ekonomik, hem de çevreye zararsız bir yolak sunmaktadır. Bitki özütleri ile yapıldığında, faydalı fitokimyasallar nanoparçacık yüzeyine tutunmakta ve nanoparçacığa işlevsellik kazandırmaktadır. Bu çalışmada, mersin yaprağı ve üzüm çekirdeğinin basit sulu özütleri kullanılarak gümüş nanoparçacıkları sentezlenmiştir. Nanoparçacıklar UV-Vis spektrofotometri, XRD, SEM ve Raman spektrofotometrisi ile karakterize edilmiştir. Ortalama boyu 10-30 nm olan küre parçacıklar elde edilmiştir. Nanoparçacıkların antibakteriyel etkisi Gram-pozitif ve Gram-negatif model organizmalarda test edilmiştir. Biyosentezle üretilen nanoparçacıkların, gelenksel yöntemle sentezlenen nanoparçacıklara veya bitki özütlerinin sulu çözeltilerine göre çok daha yüksek antibakteriyel aktivite gösterdikleri belirlenmiştir.

Keywords

• Biyosentez
• Gümüş
• Nanoparçacık
• Myrtus communis
• Üzüm çekirdeği
• Antibakteriyel

Biosynthesis and Characterization of Silver Nanoparticles Using Myrtus Communis (Myrtle) and Grape Seed Extract

Abstract

Plant based materials and extracts have gained an increased importance for biosynthesis of metallic nanoparticles. Biosynthesis offers an economical and environmentally friendly pathway for nanoparticle synthesis. When done with plant extracts, beneficial phytochemicals get absorbed on the nanoparticles and provide functionality. In this study, we have used simple water extracts of myrtle and grape seed to facilitate the synthesis of silver nanoparticles. The nanoparticles were characterized via UV-Vis spectroscopy, XRD, SEM and Raman spectroscopy. Average size of 10-30 nm spherical particles were obtained. The antibacterial activity of the nanoparticles were tested on Gram-positive and Gram-negative model organisms. Biosynthesized nanoparticles showed superior antibacterial activity compared to conventionally synthesized nanoparticles or soluble plant extracts.

Keywords

• Biosynthesis
• Silver
• Nanoparticles
• Myrtus communis
• Grapeseed
• Antibacterial

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