Journal of Agricultural Faculty of Gaziosmanpaşa University

1300-2910 2147-8848

Tokat Gaziosmanpaşa Üniversitesi

10.13002/jafag4451

Multiple Shoot and Callus Formation in Different Explants of The Medicinally Important Peganum harmala L.

Çetin

Burcu

KÜTAHYA DUMLUPINAR ÜNİVERSİTESİ Koçak

Halil İbrahim

KÜTAHYA DUMLUPINAR ÜNİVERSİTESİ

08 31 2020

37 2 77 83 04 05 2018 04 14 2020

1985

Gaziosmanpaşa Üniversitesi Ziraat Fakültesi Dergisi

In this study, the micropropagation potential of the medicinally important Peganum harmala L. plant containing valuable metabolites has been examined. For this purpose, hypocotyl and shoot tips isolated from P. harmala plants were applied different concentrations and combinations of plant growth regulators of benzyl amino purine (BAP), naphthalene acetic acid (NAA), and indole acetic acid (IAA). Best callus growth were obtained in hypocotyl explants in Murashige and Skoog (MS) medium that contained 1 mg L-1 NAA and 1 mg L-1 BAP. Best results concerning shoot count per explant (5.42±0.313 count) and shoot length (2.95±0.06 cm) were determined in MS medium which contained 1.5 mg L-1 BAP. IBA applications were performed in order to root the obtained shoots. The rooted plants were transferred to the soil with a success rate of 72%.

Peganum harmala L. plant growth regulator plant tissue culture micropropagation

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