EXPLORING EFFECTS OF INTERLEUKIN-33 ON JAK/STAT SIGNALLING PATHWAYS IN MACROPHAGES

Mehmet Zafer Kalayci; Zubeyir Elmazoglu; Vedat Bulut

EN

EXPLORING EFFECTS OF INTERLEUKIN-33 ON JAK/STAT SIGNALLING PATHWAYS IN MACROPHAGES

Abstract

It is known that IL-33 cytokine plays role in pathogenesis of some diseases. The dual role of macrophages within the tumor microenvironment is well-known and dependent on their plasticity. Macrophages mainly serve as the component of innate immune system, but they also act as a bridge between innate and acquired immune system with their antigen presentation capacity. Macrophages can change their functions by being polarized to classical M1 and alternative M2 macrophages via cytokine cocktails, such as interferon-, transforming growth factor- and interleukin-4, -10, -13. Tumor promoting effects are mainly associated with tumor-associated macrophages (TAMs) resembling the M2 types of macrophages. On the other hand, macrophages shifted to M1 type are capable of antitumour activities. Investigation of down-stream intracellular signaling pathways is very important in order to understand completely its role in pathogenesis of diseases mentioned above, as well as, to determine appropriate hypothesis in studies of therapeutic agents related to down-stream intracellular signaling pathways. This study is aimed to enlighten the effect of IL-33 on tyrosine kinase and JAK/STAT intracellular signaling pathways in macrophage cell line, J774.1. Therefore, mrIL-33 in several concentrations will be added into cell cultures of J774 stimulated by IFN-γ plus LPS or unstimulated. Then, tyrosine kinase (Tk) activities, levels and activation of Jak/STAT activities will be measured. In this study, it was observed that IL-33 additions in prior to mrIFN-γ 40 U/mL plus LPS 10 ng/mL stimulation decreased Tyk-2 levels in the group in which IL-33 levels were used at 10 ng/mL (p<0.05). In order to monitor the effects of IL-33 on tyk2, it was observed that tyrosine kinase tyk2 levels did not change when IL-33 was added to cell cultures at 10ng/mL and 100 ng/mL concentrations after 0, 4 and 18 hours. When IL-33 was applied to the J774.1 macrophage cell line at a concentration of 10 ng/mL at different times, an increase in Tyk-2 phosphorylation was observed. When IL-33 was applied to J774.1 cells at concentrations of 10 ng/mL and 100 ng/mL 4 and 18 hours before IFN-γ stimulation, statistically increased phosphorylated STAT levels were found in all IL-33 applied groups compared to the control group. These results show that high-dose IL-33 administration creates a synergistic effect on STAT activation with IFN-γ stimulation. This study will lead to observation of the effect of IL-33 in intracellular signaling pathways of macrophages. Then, it will be going through the proper channels to understand effective mechanisms of IL-33, as a therapeutic target in neoplasms.

Keywords

IL-33, intracellular signaling pathways, tyrosine kinase, JAK, STAT, INF-γ

References

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Details

Primary Language

English

Subjects

Clinical Sciences

Journal Section

Research Article

Authors

Mehmet Zafer Kalayci ^*
0000-0002-0415-6913
Türkiye

Zubeyir Elmazoglu
0000-0003-4527-8834
Türkiye

Vedat Bulut
0000-0002-0508-2127
Türkiye

Publication Date

April 30, 2022

Submission Date

April 18, 2022

Acceptance Date

April 29, 2022

Published in Issue

Year 2022 Volume: 4 Number: 1

IZ

https://izlik.org/JA89EF29DP

APA

Kalayci, M. Z., Elmazoglu, Z., & Bulut, V. (2022). EXPLORING EFFECTS OF INTERLEUKIN-33 ON JAK/STAT SIGNALLING PATHWAYS IN MACROPHAGES. Journal of Gazi University Health Sciences Institute, 4(1), 32-44. https://izlik.org/JA89EF29DP

AMA

1.Kalayci MZ, Elmazoglu Z, Bulut V. EXPLORING EFFECTS OF INTERLEUKIN-33 ON JAK/STAT SIGNALLING PATHWAYS IN MACROPHAGES. GUHES. 2022;4(1):32-44. https://izlik.org/JA89EF29DP

Chicago

Kalayci, Mehmet Zafer, Zubeyir Elmazoglu, and Vedat Bulut. 2022. “EXPLORING EFFECTS OF INTERLEUKIN-33 ON JAK STAT SIGNALLING PATHWAYS IN MACROPHAGES”. Journal of Gazi University Health Sciences Institute 4 (1): 32-44. https://izlik.org/JA89EF29DP.

EndNote

Kalayci MZ, Elmazoglu Z, Bulut V (April 1, 2022) EXPLORING EFFECTS OF INTERLEUKIN-33 ON JAK/STAT SIGNALLING PATHWAYS IN MACROPHAGES. Journal of Gazi University Health Sciences Institute 4 1 32–44.

IEEE

[1]M. Z. Kalayci, Z. Elmazoglu, and V. Bulut, “EXPLORING EFFECTS OF INTERLEUKIN-33 ON JAK/STAT SIGNALLING PATHWAYS IN MACROPHAGES”, GUHES, vol. 4, no. 1, pp. 32–44, Apr. 2022, [Online]. Available: https://izlik.org/JA89EF29DP

ISNAD

Kalayci, Mehmet Zafer - Elmazoglu, Zubeyir - Bulut, Vedat. “EXPLORING EFFECTS OF INTERLEUKIN-33 ON JAK STAT SIGNALLING PATHWAYS IN MACROPHAGES”. Journal of Gazi University Health Sciences Institute 4/1 (April 1, 2022): 32-44. https://izlik.org/JA89EF29DP.

JAMA

1.Kalayci MZ, Elmazoglu Z, Bulut V. EXPLORING EFFECTS OF INTERLEUKIN-33 ON JAK/STAT SIGNALLING PATHWAYS IN MACROPHAGES. GUHES. 2022;4:32–44.

MLA

Kalayci, Mehmet Zafer, et al. “EXPLORING EFFECTS OF INTERLEUKIN-33 ON JAK STAT SIGNALLING PATHWAYS IN MACROPHAGES”. Journal of Gazi University Health Sciences Institute, vol. 4, no. 1, Apr. 2022, pp. 32-44, https://izlik.org/JA89EF29DP.

Vancouver

1.Mehmet Zafer Kalayci, Zubeyir Elmazoglu, Vedat Bulut. EXPLORING EFFECTS OF INTERLEUKIN-33 ON JAK/STAT SIGNALLING PATHWAYS IN MACROPHAGES. GUHES [Internet]. 2022 Apr. 1;4(1):32-44. Available from: https://izlik.org/JA89EF29DP

EXPLORING EFFECTS OF INTERLEUKIN-33 ON JAK/STAT SIGNALLING PATHWAYS IN MACROPHAGES

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