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Antibacterial Effectiveness of Prophylactic Ozone Application with Full-Mouth-Tray in Orthodontic Patients with High Caries Activity

Yıl 2019, Cilt: 1 Sayı: 1, 10 - 20, 15.12.2019

Öz

The objective of this study was to investigate the
effectiveness of ozone and mouth rinse on Streptococcus mutans (S.mutans)
in pediatric patients with fixed orthodontic appliances. The study was
conducted on 13-18 years old patients with fulfilled the inclusion criteria of
high levels of S.mutans in saliva. Number of S.mutans in plaque and saliva was
determined CFU/ml terms for baseline bacteriological counts. Patients were
randomly divided into three groups: Group 1: Gaseous ozone, Group 2: Mouth
Rinse, Group 3: Daily oral hygiene regime. Immediately after applications,
stimulated saliva samples were collected again. After 1 week, plaque and saliva
samples were recorded. Mann-Whitney U, Kruskal-Wallis and Wilcoxon T tests were
used for statistical analysis. The reduced levels of S.mutans in saliva
immediately after the application in the groups of Ozone and Listerine were
found to be statistically significant (p<0,05). The Listerine group
exhibited a significantly lower S.mutans counts in saliva compared with
baseline and other groups after one week (p<0,05). After one week, the
reduced level of S.mutans in plaque as compared to baseline values in
all groups were found to be statistically significant (p<0,05). It
can be concluded that the ozone treatment may have an instantaneous lethal
effect on S.mutans; however, a long-term preventive effect could not be
observed. Listerine showed better effects on decreasing the level of S.mutans
in saliva and plaque.

Destekleyen Kurum

Gazi University Scientific Research Projects Unit

Proje Numarası

03/2012-10

Kaynakça

  • Al Shamsi, A.H., Cunningham, J.L., Lamey, P.J., & Lynch, E. (2008). The effects of ozone gas application on shear bond strength of orthodontic brackets to enamel. American Journal of Dentistry, 21(1), 35–38.
  • Alves, K.M., Goursand, D., Zenobio, E.G., & Cruz, R.A. (2010). Effectiveness of procedures for the chemical-mechanical control of dental biofilm in orthodontic patients. Journal of Contemporary Dental Practice, 11(2), 41-48.
  • Attin, R., Ilse, A., Werner, C., Wiegand, A., & Attin, T. (2006). Antimicrobial effectiveness of a highly concentrated chlorhexidine varnish treatment in teenagers with fixed orthodontic appliances. Angle Orthodontist, 76(6), 1022-1027.
  • Aykut-Yetkiner, A.A., Eden, E., Ertuğrul, F., Ergin, E., & Ateş, M. (2013). Antibacterial efficacy of prophylactic ozone treatment on patients with fixed orthodontic appliances. Acta Odontologica Scandinavica, 71(6), 1620-1624.
  • Azarpazhooh, A., & Limeback, H. (2008). The application of ozone in dentistry: a systematic review of literature., Journal of Dentistry, 36(2), 104-116.
  • Baysan, A., & Lynch, E. (2004). Effect of ozone on the oral microbiota and clinical severity of primary root caries. American Journal of Dentistry, 17(1), 56-60.
  • Castillo, A., Galindo, Moreno, P., Gustavo, A., Valderrama, M., Liebana, J., & Baca, P. (2008). In vitro reduction of mutans streptococci by means of ozone gas application. Quintessence International, 39(10), 827-831.
  • Cehreli, S.B., Guzey, A., Arhun, N., Cetinsahin, A., & Unver, B. (2010). The effects of prophylactic ozone pretreatment of enamel on shear bond strength of orthodontic brackets bonded with total or self-etch adhesive systems., European Journal of Dentistry, 4(4), 367-373.
  • Chen, Y., Wong, R.W., Seneviratne, C.J., Hagg, U., McGrath, C., & Samaranayake, L.P. (2013). The effects of natural compounds-containing mouthrinses on patients with fixed orthodontic appliance treatment: clinical and microbiological outcomes. International Journal of Paediatric Dentistry, 23(6), 452-459.
  • Derks, A., Frencken, J., Bronkhorst, E., Kuijpers-Jagtman, A.M., & Katsaros, C. (2008). Effect of chlorhexidine varnish application on mutans streptococci counts in orthodontic patients. American Journal of Orthodontics and Dentofacial Orthopedics, 133(3), 435-439.
  • Dhingra, K., & Vandana, K.L. (2011). Management of gingival inflammation in orthodontic patients with ozonated water irrigation-a pilot study. International Journal of Dental Hygiene, 9(4), 296-302.
  • Dogan, A.A., Cetin, E.S., Hüssein, E., & Adiloglu, A.K. (2009). Microbiological evaluation of octenidine dihydrochloride mouth rinse after 5 days’ use in orthodontic patients. Angle Orthodontist, 79(4), 766-777.
  • Fagrell, T., Dietz, W., Lingström, P., Steiniger, F., & Noren, J. (2008). Effect of ozone treatment on different cariogenic microorganisms in vitro. Swedish Dental Journal, 32(3), 139-147.
  • Fard, B.K., Ghasemi, M., Rastgariyan, H., Sajjadi, S.H., Emami, H., Amani, M., & Motamedi, M.H. (2011). Effectiveness of mouth washes on streptococci in plaque around orthodontic appliances. ISRN Dentistry, 2011, 1-4.
  • Fine, D.H., Furgang, D., Barnett, M.L., Drew, C., Steinberg, L., Charles, C.H., & Vincent, J.W. (2000). Effect of an essential oil-containing antiseptic mouthrinse on plaque and salivary Streptococcus mutans levels. Journal of Clinical Periodontology, 27(5), 157-161.
  • Freitas, A.O., Marquezan, M., Nojim, Mda, C., Alviano, D.S., & Maia, L.C. (2014). The influence of orthodontic fixed appliances on the oral microbiota: a systematic review. Dental Press Journal of Orthodontics, 19(2), 46-55.
  • Haas, A.N., Pannuti, C.M., Andrade, A.K., Escobar, E.C., Almeida, E.R., Costa, F.O., Cortelli, J.R., Cortelli, S.C., Rode, S.D., Pedrazzi, V., & Oppermann, R.V. (2014). Mouthwashes for the control of supragingival biofilm and gingivitis in orthodontic patients: evidence-based recommendations for clinicians. Brazilian Oral Research, 28, 1-8.
  • Jensen, B., & Brathall, D. (1989). A new method for the estimation of mutans streptococci human saliva. Journal of Dental Research, 68(3), 468-471.
  • Johanson, E., Claesson, R., & van Dijken, J.W.V. (2009). Antibacterial effect of ozone on cariogenic bacterial species. Journal of Dentistry, 37(6), 449-453.
  • Kapdan, A., Öztaş, N., & Sümer, Z. (2013). Comparing the antibacterial activity of gaseous ozone and chlorhexidine solution on a tooth cavity model. Journal of Clinical and Experimental Dentistry, 5(3), e133.
  • Koopman, J.E., van der Kaaij, N.C., Bujis, M.J., Elyassi, Y., van der Veen, M.H., Crielaard, W., Ten, Cate, J.M., & Zaura, E. (2015). The effect of fixed orthodontic appliances and fluoride mouthwash on the oral microbiome of adolescents- A randomized controlled clinical trial. Plus ONE, 10, e0137318.
  • Kronenberg, O., Lussi, A., & Ruf, S. (2009). Preventive effect of ozone on the development of white spot lesions during multibracket appliance therapy. Angle Orthodontist, 79(1), 64–69.
  • Marchetti, E., Mummolo, S., Di Mattia, J., Casalena, F., Di Martino, S., Mattei, A., & Marzo, G. (2011). Efficacy of essential oil mouthwash with and without alcohol: a 3-day plaque accumulation model. Trials, 12(1), 262.
  • Migliorati, M., Isaia, L., Cassaro, A., Rivetti, A., Silvestrini-Biavati, F., Gastaldo, L., Piccardo, I., Dalessandri, D., & Silvestrini-Biavati, A. (2015). Efficacy of professional hygiene and prophylaxis on preventing plaque increase in orthodontic patients with multibracket appliances: a systematic review. European Journal of Orthodontics, 37(3), 297-307.
  • Oltramari-Navarro, P.V.P., Titarelli, J.M., Marsicano, J.A., Henriques, J.F.C., Janson, G., Lauris, J.R.P., & Buzalaf, M.A.R. (2009). Effectiveness of 0.50% and 0.75% chlorhexidine dentifrices in orthodontic patients: a double-blind and randomized controlled trial. American Journal of Orthodontics and Dentofacial Orthopedics, 136(5), 651-656.
  • Perrini, F., Lombardo, L., Arreghini, A., Medori, S., & Siciliani, G. (2016). Caries prevention during orthodontic treatment: In-vivo assessment of high-fluoride varnish to prevent white spot lesions. American Journal of Orthodontics and Dentofacial Orthopedics, 149(2), 238-243.
  • Pithan, M.M., Sant’Anna, L.I., Baiâo, F.C., dos Santos, R.L., Coqueiro, Rda, S., & Maia, L.C. (2015). Assessment of the effectiveness of mouthwashes in reducing cariogenic biofilm in orthodontic patients: A systematic review. Journal of Dentistry, 43(3), 297-308.
  • Polydorou, O., Halili, A., Wittmer, A., Pelz, K., & Hahn, P. (2012). The antibacterial effect of gas ozone after 2 months of in vitro evaluation. Clinical oral investigations, 16(2), 545- 550.
  • Sari, E., & Birinci, I. (2007). Microbiological evaluation of 0.2% chlorhexidine gluconate mouth rinse in orthodontic patients. Angle Orthodontist, 77(5), 881-884.
  • Topaloglu-Ak, A., Ertugrul, F., Eden, E., Ates, M., & Bulut, H. (2011). Effect of orthodontic appliances on oral microbiota—6 month follow-up. Journal of Clinical Pediatric Dentistry, 35(4), 433-436.
  • Tufekci, E., Casagrande, Z. A., Lindauer, S. J., Fowler, C. E., & Williams, K. T. (2008). Effectiveness of an essential oil mouthrinse in improving oral health in orthodontic patients. The Angle Orthodontist, 78(2), 294-298.
  • Wikén Albertsson, K., Persson, A., & Van Dijken, J. W. (2013). Effect of essential oils containing and alcohol-free chlorhexidine mouthrinses on cariogenic micro-organisms in human saliva. Acta Odontologica Scandinavica, 71(3-4), 883-891.
Yıl 2019, Cilt: 1 Sayı: 1, 10 - 20, 15.12.2019

Öz

Proje Numarası

03/2012-10

Kaynakça

  • Al Shamsi, A.H., Cunningham, J.L., Lamey, P.J., & Lynch, E. (2008). The effects of ozone gas application on shear bond strength of orthodontic brackets to enamel. American Journal of Dentistry, 21(1), 35–38.
  • Alves, K.M., Goursand, D., Zenobio, E.G., & Cruz, R.A. (2010). Effectiveness of procedures for the chemical-mechanical control of dental biofilm in orthodontic patients. Journal of Contemporary Dental Practice, 11(2), 41-48.
  • Attin, R., Ilse, A., Werner, C., Wiegand, A., & Attin, T. (2006). Antimicrobial effectiveness of a highly concentrated chlorhexidine varnish treatment in teenagers with fixed orthodontic appliances. Angle Orthodontist, 76(6), 1022-1027.
  • Aykut-Yetkiner, A.A., Eden, E., Ertuğrul, F., Ergin, E., & Ateş, M. (2013). Antibacterial efficacy of prophylactic ozone treatment on patients with fixed orthodontic appliances. Acta Odontologica Scandinavica, 71(6), 1620-1624.
  • Azarpazhooh, A., & Limeback, H. (2008). The application of ozone in dentistry: a systematic review of literature., Journal of Dentistry, 36(2), 104-116.
  • Baysan, A., & Lynch, E. (2004). Effect of ozone on the oral microbiota and clinical severity of primary root caries. American Journal of Dentistry, 17(1), 56-60.
  • Castillo, A., Galindo, Moreno, P., Gustavo, A., Valderrama, M., Liebana, J., & Baca, P. (2008). In vitro reduction of mutans streptococci by means of ozone gas application. Quintessence International, 39(10), 827-831.
  • Cehreli, S.B., Guzey, A., Arhun, N., Cetinsahin, A., & Unver, B. (2010). The effects of prophylactic ozone pretreatment of enamel on shear bond strength of orthodontic brackets bonded with total or self-etch adhesive systems., European Journal of Dentistry, 4(4), 367-373.
  • Chen, Y., Wong, R.W., Seneviratne, C.J., Hagg, U., McGrath, C., & Samaranayake, L.P. (2013). The effects of natural compounds-containing mouthrinses on patients with fixed orthodontic appliance treatment: clinical and microbiological outcomes. International Journal of Paediatric Dentistry, 23(6), 452-459.
  • Derks, A., Frencken, J., Bronkhorst, E., Kuijpers-Jagtman, A.M., & Katsaros, C. (2008). Effect of chlorhexidine varnish application on mutans streptococci counts in orthodontic patients. American Journal of Orthodontics and Dentofacial Orthopedics, 133(3), 435-439.
  • Dhingra, K., & Vandana, K.L. (2011). Management of gingival inflammation in orthodontic patients with ozonated water irrigation-a pilot study. International Journal of Dental Hygiene, 9(4), 296-302.
  • Dogan, A.A., Cetin, E.S., Hüssein, E., & Adiloglu, A.K. (2009). Microbiological evaluation of octenidine dihydrochloride mouth rinse after 5 days’ use in orthodontic patients. Angle Orthodontist, 79(4), 766-777.
  • Fagrell, T., Dietz, W., Lingström, P., Steiniger, F., & Noren, J. (2008). Effect of ozone treatment on different cariogenic microorganisms in vitro. Swedish Dental Journal, 32(3), 139-147.
  • Fard, B.K., Ghasemi, M., Rastgariyan, H., Sajjadi, S.H., Emami, H., Amani, M., & Motamedi, M.H. (2011). Effectiveness of mouth washes on streptococci in plaque around orthodontic appliances. ISRN Dentistry, 2011, 1-4.
  • Fine, D.H., Furgang, D., Barnett, M.L., Drew, C., Steinberg, L., Charles, C.H., & Vincent, J.W. (2000). Effect of an essential oil-containing antiseptic mouthrinse on plaque and salivary Streptococcus mutans levels. Journal of Clinical Periodontology, 27(5), 157-161.
  • Freitas, A.O., Marquezan, M., Nojim, Mda, C., Alviano, D.S., & Maia, L.C. (2014). The influence of orthodontic fixed appliances on the oral microbiota: a systematic review. Dental Press Journal of Orthodontics, 19(2), 46-55.
  • Haas, A.N., Pannuti, C.M., Andrade, A.K., Escobar, E.C., Almeida, E.R., Costa, F.O., Cortelli, J.R., Cortelli, S.C., Rode, S.D., Pedrazzi, V., & Oppermann, R.V. (2014). Mouthwashes for the control of supragingival biofilm and gingivitis in orthodontic patients: evidence-based recommendations for clinicians. Brazilian Oral Research, 28, 1-8.
  • Jensen, B., & Brathall, D. (1989). A new method for the estimation of mutans streptococci human saliva. Journal of Dental Research, 68(3), 468-471.
  • Johanson, E., Claesson, R., & van Dijken, J.W.V. (2009). Antibacterial effect of ozone on cariogenic bacterial species. Journal of Dentistry, 37(6), 449-453.
  • Kapdan, A., Öztaş, N., & Sümer, Z. (2013). Comparing the antibacterial activity of gaseous ozone and chlorhexidine solution on a tooth cavity model. Journal of Clinical and Experimental Dentistry, 5(3), e133.
  • Koopman, J.E., van der Kaaij, N.C., Bujis, M.J., Elyassi, Y., van der Veen, M.H., Crielaard, W., Ten, Cate, J.M., & Zaura, E. (2015). The effect of fixed orthodontic appliances and fluoride mouthwash on the oral microbiome of adolescents- A randomized controlled clinical trial. Plus ONE, 10, e0137318.
  • Kronenberg, O., Lussi, A., & Ruf, S. (2009). Preventive effect of ozone on the development of white spot lesions during multibracket appliance therapy. Angle Orthodontist, 79(1), 64–69.
  • Marchetti, E., Mummolo, S., Di Mattia, J., Casalena, F., Di Martino, S., Mattei, A., & Marzo, G. (2011). Efficacy of essential oil mouthwash with and without alcohol: a 3-day plaque accumulation model. Trials, 12(1), 262.
  • Migliorati, M., Isaia, L., Cassaro, A., Rivetti, A., Silvestrini-Biavati, F., Gastaldo, L., Piccardo, I., Dalessandri, D., & Silvestrini-Biavati, A. (2015). Efficacy of professional hygiene and prophylaxis on preventing plaque increase in orthodontic patients with multibracket appliances: a systematic review. European Journal of Orthodontics, 37(3), 297-307.
  • Oltramari-Navarro, P.V.P., Titarelli, J.M., Marsicano, J.A., Henriques, J.F.C., Janson, G., Lauris, J.R.P., & Buzalaf, M.A.R. (2009). Effectiveness of 0.50% and 0.75% chlorhexidine dentifrices in orthodontic patients: a double-blind and randomized controlled trial. American Journal of Orthodontics and Dentofacial Orthopedics, 136(5), 651-656.
  • Perrini, F., Lombardo, L., Arreghini, A., Medori, S., & Siciliani, G. (2016). Caries prevention during orthodontic treatment: In-vivo assessment of high-fluoride varnish to prevent white spot lesions. American Journal of Orthodontics and Dentofacial Orthopedics, 149(2), 238-243.
  • Pithan, M.M., Sant’Anna, L.I., Baiâo, F.C., dos Santos, R.L., Coqueiro, Rda, S., & Maia, L.C. (2015). Assessment of the effectiveness of mouthwashes in reducing cariogenic biofilm in orthodontic patients: A systematic review. Journal of Dentistry, 43(3), 297-308.
  • Polydorou, O., Halili, A., Wittmer, A., Pelz, K., & Hahn, P. (2012). The antibacterial effect of gas ozone after 2 months of in vitro evaluation. Clinical oral investigations, 16(2), 545- 550.
  • Sari, E., & Birinci, I. (2007). Microbiological evaluation of 0.2% chlorhexidine gluconate mouth rinse in orthodontic patients. Angle Orthodontist, 77(5), 881-884.
  • Topaloglu-Ak, A., Ertugrul, F., Eden, E., Ates, M., & Bulut, H. (2011). Effect of orthodontic appliances on oral microbiota—6 month follow-up. Journal of Clinical Pediatric Dentistry, 35(4), 433-436.
  • Tufekci, E., Casagrande, Z. A., Lindauer, S. J., Fowler, C. E., & Williams, K. T. (2008). Effectiveness of an essential oil mouthrinse in improving oral health in orthodontic patients. The Angle Orthodontist, 78(2), 294-298.
  • Wikén Albertsson, K., Persson, A., & Van Dijken, J. W. (2013). Effect of essential oils containing and alcohol-free chlorhexidine mouthrinses on cariogenic micro-organisms in human saliva. Acta Odontologica Scandinavica, 71(3-4), 883-891.
Toplam 32 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Diş Hekimliği
Bölüm Makaleler
Yazarlar

Nagehan Aktaş

Didem Atabek

Gülçin Akça

Nurhan Öztaş

Proje Numarası 03/2012-10
Yayımlanma Tarihi 15 Aralık 2019
Yayımlandığı Sayı Yıl 2019 Cilt: 1 Sayı: 1

Kaynak Göster

APA Aktaş, N., Atabek, D., Akça, G., Öztaş, N. (2019). Antibacterial Effectiveness of Prophylactic Ozone Application with Full-Mouth-Tray in Orthodontic Patients with High Caries Activity. Journal of Gazi University Health Sciences Institute, 1(1), 10-20.