Fourier Transform Infrared (FT-IR) Spectroscopy for Biological Studies(REVIEW)

Year 2009, Volume: 22 Issue: 3, 117 - 121, 24.03.2010

Dilek Duygu Tülay Baykal İlkay Açıkgöz Kazım Yıldız

Abstract

In the recent years, vibrational spectroscopies (infrared spectroscopy) have been developed for all sorts of analysis in microbiology. Important features of these methods are the relative ease with which measurements can be performed. Fourier transform infrared spectroscopy (FT-IR) is a technique that has been used over the years in chemical analysis for the identification of substances and is one that may be applied to the characterization of microorganisms. Infrared spectroscopy is based on the measurement of the molecular bond vibration compounds, excited by radiation of a suitable frequency, when given the conditions for energy absorption by the molecules. Here, we review the potential application of vibrational spectroscopies for use in biology. It can be concluded that vibrational spectroscopy show high potential as novel methods.

Key Words: Fourier Transforms Infrared Spectroscopy, Microorganisms, Identification, Classification, Discrimination.

 

Keywords

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References

• Margarita P., Quinteiro R., “Fourier Transform Infrared (FT-IR) Technology for the Identification of Organisms”, Clinical Microbiology Newsletter, (22), No.8 (2000).
• Greensteet, J.E., Norris, K.P., “The existence of differences between the infrared absorption spectra of bacteria”, Spectrochim. Acta, 9: 177-182
• Goulden, J.D.S., Sharpe, M.E., “The infrared absorption J.Gen.Microbiol., 19: 76-86 (1958). of lactobacilli”,
• Jackson, M., Mantsch, H., “Biomedical infrared spectroscopy”, In: H.H. Mantsch and D. Chapman (eds.), Infrared spectroscopy of biomolecules, Wiley-Liss, NY (1996).
• Riddle, J.W. et al., “Bacterial identification by infrared spectrophotometry”, J.Bacteriol, 72: 593- 603 (1956).
• Maquelin, K., Kirschner, C., “Identification of medically relevant microorganisms by vibrational spectroscopy”, Methods, 51: 255-271 (2002). of Microbiological
• Naumann, D., Fijala, V., Labischinski, H., “The rapid pathogenic bacteria using Fourier Transform Infrared spectroscopic and multivariate statistical analysis”, J.Mol.Struct., 174: 165-170 (1988). of
• Gomez, M.A., Perez, M.A.B., Gil, F.J.M., “Identification of species of Brucella using Fourier transform infrared spectroscopy”, Journal of Microbiological Methods, 55: 121-131 (2003).
• Lipkus, A.H., Chittur, K.K., Vesper, S.J., “Evolution of infrared spectroscopy as a bacterial identification method”, J. Ind.Microbiol., 6: 71-75 (1990).
• Curk, M.C., Peledan, F., Hubert, J.C., “Fourier Transform infrared (FT-IR) spectroscopy for identifying Microbiol. Lett., 123: 241-248 (1994). species”, FEMS
• Siebert, F., “Infrared spectroscopy applied to biochemical and biological problems”, In: Sauer, K. (Ed), Biochemical Spectroscopy, Methods in Enzymology, 246: 501-526 (1995).
• Jackson, M., Sowa, M.G., “Infrared spectroscopy: a new frontier in medicine”, Biophys. Chem., 68: 109-125 (1997).
• Diem, M., Boydston-White, “Infrared spectroscopy of cells and tissues: shining light onto a novel subject”, Appl. Sepctrosc., 53: 148-161 (1999).
• Wenning, M., Seiler, H., Scherer, S., “Fourier- transform infrared microspectroscopy, a novel and rapid tool for identification of yeast”, Applied and Environmental Microbiology, 68(10): 4717-4721 (2002).
• Sacksteder, C., Barry, B.A., “Fourier Transform Infrared Spectroscopy: A Molecular Approach to an Organismal Question”, Journal of Phycology, 37: 197-199 (2001).
• Naumann, D., “Infrared and NIR Raman spectroscopy in medical microbiology”, In: Manch, H.H., Jackson, M. (Eds.), Infrared Spectroscopy: New Tool in Medicine, SPIE Proceeding Series (3257), SPIE, Bellingham, WA, 245-257 (1998).
• Naumann, D., et al., “The characterization of microorganisms by Fourier-transform infrared spectroscopy (FT-IR)”, In W.H. Nelson (ed.), Modern techniques for rapid microbilogical analysis, VCH, NY (1991).
• Montechaiaro, F., Hirschmugl, C.J., Raven, J.A., Giordano, M., “Homeostasis of cell composition during prolonged darkness”, Plant, Cell and Environment, 29: 2198-2204 (2006).
• Sekkal, M., Legrand, P., Huvenne, J.P., Verdus, M.C., “The use of FTIR microspectormetry as a tool for the identification in situ of polygalactanes in red seaweeds”, Journal of Molecular Structure, 294: 227-230 (1993).
• Benning, L.G., Phoenix, V.R., Yee, N., Tobin, M.J., cyanobacterial infrared micro-spectroscopy”, Geochimica et Cosmochimica Acta, 68(4): 729-741 (2004). of silification using synchrotron
• Giordano, M., Kansiz, M., Heraud, P., Beardall, J., Wood, B., McNaughton, D., “Fourier transform infrared spectroscopy as a novel tool to investigate changes in intracellular macromolecular pools in the marine microalgae Chaetoceros mullerii (Bacillariophyceae)”, J.Phycol., 37: 271-279 (2001).
• Dean, A.P., Sigee, D.C., “Molecular heterogeneity in Aphanizomenon flos-aquae and Anabaena flos- aquae (Cyanophyta): a synchrotron-based fourier- transform micropopulations”, Eur.J.Phycol., 41(2): 201-212 (2006). study of lake [23] Haag, H., et of and FTIR identification spectroscopy”, J. Microbiol. Methods, 27: 157- 163 (1996). by
• Kos, G., Lohninger, H., Krska, R., “Fourier transform attenuated total (FT-IR\ATR) as a tool for the detection Vibrational Spectroscopy, 29: 115-119 (2002). fungi on maize”,
• Fischer, G., et al, “FT-IR spectroscopy as a tool for characterization of airborne filamentous fungi”, Journal of Microbiological Methods, 64: 63-77
• Oust, A., et al., “FT-IR spectroscopy for identification of closely related lactobacilli”, Journal of Microbiological Methods, 59: 149-162 (2004).
• Naumann, D., et al., “What can infrared spectroscopy tell us about the structure and composition of intact bacterial cells?”, In H.H. Mantsch and D. Chapman (eds.), Infrared spectroscopy of biomolecules, Wiley-Liss, NY (1996).
• Schuster, K.C., Mertens, F., Gapes, J.R., “FT-IR spectroscopy applied to bacterial cells as a novel method for monitoring complex biotechnological processes”, Vibrational Spectroscopy, 19: 467-477 (1999).
• Irudayaraj, J., Yang, H., Sakhamuri, S., “Differentiation and detection of microorganisms using Fourier transform infrared photoacoustic spectroscopy”, Journal of Molecular Structure, 606: 181-188 (2002). [30] Naumann, D., Helm, D., “Microbiological
• characterizations by Fourier transform infrared
• spectroscopy”, Nature, 351: 81-82 (1991).
• Orsini, F., Ami, D., Villa, A.M., “FT-IR microspectroscopy for microbiological studies”, Journal of Microbiological Methods, 42: 17-24 (2000).
• Helm, D., Naumann D., “Identification of some bacterial cell components by FT-IR spectroscopy”, FEMS Microbiology Letters, 126: 75-80 (1995).