A Histochemical Study of Glycoconjugates in the Gills of the Sea Bass (Dicentrarchus labrax L. 1758)

Yıl 2009, Cilt: 22 Sayı: 4, 257 - 261, 25.03.2010

Dilek Diler Kenan Çınar

Öz

The characteristics of glycoconjugates in the mucous cells of the sea bass (Dicentrarchus labrax L. 1758) gill were investigated by histochemical staining procedures. Histochemical results showed that mucous cells in the primary filament (primary lamellae) epithelium contain acidic, neutral and sulphated glycoconjugates and also mucous cells were stained weakly with AB pH 0.5 were detected in the primary filament epithelium.

 

Key Words: Dicentrarchus labrax, Gill, Glycoconjugates, Histochemistry, Mucous cells.

Anahtar Kelimeler

-

Kaynakça

• [1] Fosket, K.J., Bern, H.A., Machen, T.E., Conner, M., “Chloride cells and the hormonal control of teleost fish osmoregulation”, Journal of Experimental Biology, 106: 255-281 (1983).
• [2] Laurent, P., “Gill Internal Morphology”, In: Hoar, W.S., Randall, D.J. (Eds.), Fish Physiology, Academic Press, New York, London, 73-183 (1984).
• [3] Laurent, P., Dunel-Erb, S., Chevalier, C., Lignon, J., “Gill epithelial cell kinetics in a freshwater teleost Oncorhynchus mykiss during adaptation to ion-poor water and hormonal treatment”, Fish Physiology and Biochemistry, 3: 353-370 (1994).
• [4] Goss, G.G., Perry, S.F., Fryer, J.N., Laurent, P., “Gill morphology and acid–base regulation in freshwater fishes- effects of environmental pH change and sodium uptake blockade”, Comparative Biochemistry and Physiology A, 119: 107-115 (1998).
• [5] Evans, D.H., Piermarini, P.M., Potts, W.T.W., “Ionic transport in the fish gill epithelium”, Journal of Experimental Zoology, 283: 641-652 (1999).
• [6] Carmona, R., Garcia-Gallego, M., Sanz, A., Domezain, A., Ostos-Garrido, M.V., “Chloride cells and pavement cells in gill epithelia of Acipenser naccarii: ultrastructural modifications in seawater-acclimated specimens”, Journal of Fish Biology, 64: 553-566 (2004).
• [7] Diaz, A.O., Garcia, A.M., Devincenti, C.V., Goldemberg, A.L., “Ultrastructure and histochemical study of glycoconjugates in the gills of the white croaker (Micropogonias furnieri)”, Anatomia Histologia Embryologia-Journal of Veterinary Medicine Series C, 34: 117-22 (2005).
• [8] Fletcher, T.C., “Defence mechanism in fish”, Journal of Histochemistry&Cytochemistry, 32: 681-689 (1978).
• [9] Shephard, K.L., “Functions for fish mucus”, Reviews in Fish Biology and Fisheries, 4: 401- 429 (1994).
• [10] Domeneghini, C., Ponnelli Straini, R., Veggetti, A., “Gut glycoconjugates in Sparus aurata L. (Pisces Teleostei). A comparative histochemical study in larval and adult ages”, Histology and Histopathology, 13: 359-72 (1998).
• [11] Diaz, A.O., Garcia, A.M., Devincenti, C.V., Goldemberg, A.L., “Mucous cells in Micropogonias furnieri gills: Histochemistry and ultrastructure”, Anatomia Histologia Embryologia-Journal of Veterinary Medicine Series C, 30: 135-139 (2001).
• [12] Strous, G.J., Dekker, J., “Mucin-type glycoproteins”, Critical Reviews in Biochemistry and Molecular Biology, 27: 57-92 (1992).
• [13] Sarasquete, C., Gonzáles de Canales, M.L., Arellano, J., Muñoz Cueto, J.A., Ribeiro, L., Dinis, M.T., “Histochemical study of skin and gills of Senegal sole, Solea senegalensis larvae and adults”, Histology and Histopathology, 13: 727- 735 (1998).
• [14] Calabró, C., Albanese, M.P., Lauriano, E.R., Licata, A., Martella, S., Concetta, C., “Morphological histochemical and immunohistochemical study of the gill epithelium in the abyssal teleost fish Coelorhynchus coelorhynchus”, Folia Histochemica Et Cytobiologica, 43: 51-56 (2005).
• [15] Çınar, K., Şenol, N., Özen, M.R., “Histochemical characterization of glycoproteins in the gills of the carp (Cyprinus carpio)”, Ankara Üniversitesi Veteriner Fakültesi Dergisi, 55: 61-64 (2008).
• [16] McManus, J.F.A., “Histological and histochemical uses of periodic acid”, Stain Technology, 23: 99- 108 (1948).
• [17] Mowry, R.W., “Alcian blue techniques for the histochemical study of acidic carbohydrates”, Journal of Histochemistry&Cytochemistry, 4: 407-408 (1956).
• [18] Lev, R., Spicer, S.S., “Specific staining of sulphate groups with alcian blue at low pH”, Journal of Histochemistry&Cytochemistry, 12: 309-309 (1964).
• [19] Gomori, G., “Gomori’ s Aldehyde Fuchsin Stain”, In: Culling, C.F.A., Allison, R.T., Barr, W.T. (Eds.), Cellular Pathology Technique. Butterworths, London, 238 (1952).
• [20] Spicer, S.S., Mayer, D.R. “Aldehyde Fuchsin/Alcian Blue”, In: Culling, C.F.A., Allison, R.T., Barr, W.T. (Eds.), Cellular Pathology Technique, Butterworths, London, 233 (1960).
• [21] Diaz, A.O., Garcia, A.M., Devincenti, C.V., Goldemberg, A.L., “Glycoconjugates in the branchial mucous cells of Cynoscion guatucupa (Cuvier, 1830) (Pisces: Sciaenidae)”, Scientia Marina, 69: 545-553 (2005).
• [22] Arellano, J.M., Storch, V., Sarasquete, C., “Ultrastructure and histochemical study on gills and skin of the Senegal sole Solea senegalensis”, Journal of Applied Ichthyology, 20: 452-460 (2004)