A TAXONOMIC STUDY ON ORTHRIAS INSIGNIS EUPHRATICUS (Banarescu and Nalbant, 1964) and CYPRINION MACROSTOMUS (Heckel, 1843) by SARCOPLASMIC PROTEIN ELECTROPHORESIS

Year 2005, Volume: 18 Issue: 1, 61 - 68, 17.08.2010

Muhittin Yılmaz , Yılmaz Çiğremiş Yusuf Türköz Muhammed Gaffaroğlu

Abstract

In this study, SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) was applied to the sarcoplasmic proteins of Orthrias insignis euphyraticus (BALITORIDAE) and Cyprinion macrostomus (CYPRINIDAE) fish taken from Karakaya Dam Lake. The electrophoregram showed that there were differences between the two species in both the number of bands and the molecular weight (MW) of the sarcoplasmic proteins. While Cyprinion macrostomus had 18 protein bands, there were 20 protein bands in Orthrias insignis euphyraticus. On the other hand, the bands obtained from Orthrias insignis euphyraticus and numbered as 9th protein band with molecular weight of 29.5 kD and 12th band with MW of 27.2 kD were not found in Cyprinion macrostomus. 9th protein band with MW of 27.5 kD was only found in Cyprinion macrostomus. We concluded that comparisons of sarcoplasmic proteins might have practical importance in taxonomic classification.

Keywords

Key Words: Fish, Sarcoplasmic Proteins, SDS-PAGE, Taxonomy

SARKOPLAZMİK PROTEİN ELEKTROFOREZİ İLE ORTHRIAS INSIGNIS EUPHRATICUS (Banarescu and Nalbant, 1964) ve CYPRINION MACROSTOMUS (Heckel, 1843) TAKSONOMİSİNİN İNCELENMESİ

Abstract

Bu çalışmada, SDS-PAGE (sodyum dodesil sülfat-poliakrilamid jel elektroforezi)’ne, Karakaya Baraj Gölü’nden yakalanan Orthrias insignis euphyraticus (BALITORIDAE) ve Cyprinion macrostomus (CYPRINIDAE) balıklarının sarkoplazmik proteinleri uygulandı. Elektroforegramda, sarkoplazmik proteinlerin band sayısı ve molekül ağırlıklarının iki tür arasında farklı olduğu görüldü. Cyprinion macrostomus 18 protein bandına sahipken Orthrias insignis euphyraticus’da 20 protein bandı vardı. Diğer taraftan, Orthrias insignis euphyraticus’dan elde edilen 29.5 kD moleküler ağırlığına sahip 9. protein bandı ve 27.2 kD moleküler ağırlığına sahip 12. protein bandı Cyprinion macrostomus’ta bulunmadı. 27.5 kD moleküler ağırlığına sahip 9. protein bandı yalnızca Cyprinion macrostomus’ta bulundu. Sarkoplazmik proteinlerin karşılaştırılmasının taksonomik sınıflandırmada pratik önemi olabileceğine karar verildi

Keywords

Balık, Sarkoplazmik proteinler, SDS-PAGE, Taksonomi

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