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Investigation of Digestive Tract of Sphex flavipennis Fabrius, 1793 (Hymenoptera: Sphecidae): Morphology and Ultrastructure

Year 2011, Volume: 24 Issue: 3, 397 - 405, 25.11.2011

Abstract

 

In this study, the ultrastructure and histology of digestive tract of Sphex flavipennis was examined by light and electron microscopy. The digestive tract divided into foregut, midgut and hindgut. There are longitudinal and circular muscle in the haemocoel side and a cuticle in the luminal side of epithelium. The epithelium made of three kinds of cells; endocrine cells, regenerative cells and enterocytes. The cytoplasm of endocrine cells filled with secretory granules and lacked infoldings of basal plasma membranes. The regenerative cells are small, undifferentiating cells. The enterocytes have well developed infoldings of basal plasma membrane that are adjacent with mitochondria.

 

Key words: Sphex flavipennis, Digestive tract, Ultrastructure, Morphology

References

  • [1] Billen, J. and Buschinger, A., “Morphology and ultrastructure of a specialized bacterial pouch in the digestive tract of Tetraponera ants (Formicidae, Pseudomyrmecinae)”, Arthropod Structure and Development, 29: 259-266, (2000).
  • [2] Dow, J.A.T., “Insect midgut function”, Adv. Insect Physiol., 19: 187-328, (1986).
  • [3] Boonsriwong, W., Sukontason, K., Olson, J.K., Vogtsberger, R.C., Chaithong, U., Kuntalue, B., Ngern-klun, R., Upakut, S. and Sukontason, K.L., “Fine structure of the alimentary canal of the larval blow fly Chrysomya megacephala (Diptera: Calliphoridae)”, Parasitol. Res., 100: 561-574, (2007).
  • [4] Terra, W.R. and Ferrreira, C., “Insect digestive enzymes: Properties, compartmentalizasyon and function”, Com. Biochem. Physiol. B, 109: 1-62, (1994).
  • [5] Levy, S.M., Falleiros, Â.M.F., Moscardi, F., Gregório, E.A. and Toledo, L.A., “Morphological study of the hindgut in larvae of Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae)”, Neotropical Entomology, 33: 427-431, (2004).
  • [6] Uwo, M.F., Ui-Tei, K. and Takeda, P.P.M., “Replacement of midgut epithelium in the greater wax moth, Galleria mellonela, during larval-pupal moult”, Cell Tissue Res., 308: 319-331, (2002).
  • [7] Serrão, J.E., “A comparative study of the proventricular structure in corbiculate apinae (Hymenoptera, Apidae)”, Micron, 32: 379-385, (2001).
  • [8] Serrão, J.E., “Proventricular structure in the bee tribe Augochlorini (Hymenoptera: Halictidae)”, Organisms, Diversity and Evolution, 7: 175-180, (2007).
  • [9] Ameen, M. and Imam, F., “Alimentary canal of Sphaerodema rusticum (F.) (Hemiptera: Belostomatidae)”, J. Nat. History, 10: 545-568, (1976).
  • [10] Santos, C.D., Ribeiro, A.F., Ferreira, C. and Terra, W.R., “The larval midgut of cassava hornworrm (Erinnyyis ello); Ultrastructure, fluid fluxes and secretory activity in relation to the organization of digestion”, Tissue Res., 237: 565- 574, (1984).
  • [11] Anderson, E. and Harvey, W.R., “Active transport by Cecropia midgut. II. Fine structure of the midgut epithelium”, J. Cell Biol., 31: 107- 134, (1966).
  • [12] Rost, M.M., “Ultrastructural changes in the midgut epithelium in Podura aquatica L. (Insecta, Collembola, Arthropleona) during regeneration”, Arthropod Structure and Development, 35: 69-76, (2006).
  • [13] Billingsley, P.F., “The midgut ultrastructure of hematophagous insects”, Ann. Rev. Entomol., 35: 219-248, (1990).
  • [14] Garcia, J.J., Li, G., Wang, P., Zhong, J. and Granados, R.R., “Primary and continuous midgut cell cultures from Pseudaletia unipuncata (Lepidoptera, Noctuidae)”, In Vitro Cell. Develop. Biol., 37: 353-359, (2001).
  • [15] Sadrud-Din, S., Loeb, M. and Hakim, R., “In vitro differentiation of isolated stem cells from the midgut of Manduca sexta larvae”, J. Exp. Biol., 199, 319–325, (1996).
  • [16] Hakim, R.S., Baldwin, K.M. and Loeb, M., “The role of stem cells in midgut growth and regeneration”, In Vitro Cell. Develop. Biol., 37, 338–342 (2001).
  • [17] Brown, M.R., Raikhel, A.S. and Lea, A.O., “Ultrastructure of midgut endocrine cells in the adult mosquito, Aedes aegypti”, Tissue Cell, 17:709-721, (1985).
  • [18] Serrão, J.E. and Cruz-Landim, C., “Ultrastructure of midgut endocrine cells in workers of stingless bee (Hymenoptera, Apidae, Meliponinae)”. Iheringia, Série Zoologia, 81:151-156, (1996).
  • [19] Neves, C.A., Bhering, J.E., Serrão, J.E. and Gitirana, L.B., “FMRFamide-like midgut endocrine cells during the metamorphosis in Melipona quadrifasciata anthidioides Hymenoptera, Apidae)”, Micron, 33:453-460 (2002).
  • [20] Peacock, A.J., “Ultrastructure of ilrum of Locusta migratoria”, J. Morphol., 188: 191-201 (1986).
  • [21] Bution, M.L., Caetano, F.H., Britto, F.B., Tomaino Gomes, G.A. and Zara, F.J., “Histology and histochemistry of ventriculus of Dolichoderus (=Monacis) bispinosus (Oliver, 1792) (Hymenoptera: Formicidae)”, Micron: 249-254, (2006).
  • [22] Baker, J.E., Woo, S.M. and Byrd, R.V., “Ultrastructural features of the gut Sitophilus granarius (L.) (Coleoptera: Curculionidae) with notes on distribution of proteinases and amylases in crop and midgut”. Can. J. Zool., 62: 1251-1259, (1984).
  • [23] Roche, R.K. and Wheeler, D.E., “Morphological specializations of the digestive tract of Zacryptocerus rohweri (Hymenoptera: Formicidae)”, J. Morphol., 234: 253-262, (1997).
  • [24] Serrão, J.E. and Cruz-Landim, C., “Scanning electronic microscopy of proventriculus in stingless bees (Apidae: Meliponinae) with a comparision of necrophagus and feeding pollen workers”, Naturalia, 20: 207-212, (1995).
  • [25] Serrão, J.E., “A comparative study of the proventricular structure in corbiculate Apinae (Hymenoptera, Apidae)”, Micron, 32: 379-386 (2000).
  • [26] Hung, C., Lin, T. and Lee, W., “Morphology and ultrastructure of the alimentary canal of the oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae) (2): The structure of the midgut”, Zoological Studies, 39: 387-394, (2000).
  • [27] Raes, H. and Verbeke, M., “Light and electron microscopical study of two types of endocrine cell in the midgut of the adult worker honeybee (Apis mellifera)”, Tissue Cell, 26: 223-230, (1994).
  • [28] Pigino, G., Migliorini, M., Paccagnini, E., Bernini, F. and Leonzio, C., “Fine structure of the midgut Malpighian papillae in Campodea (Monocampa) quilisi Silvestri, 1932 (Hexapoda, Diplura) with special reference to the metal composition and physiological significance of midgut intracellular electron-dense granules”, Tissue Cell, 37: 223-232, (2005).
  • [29] Andrade-Coêlho, C.A., Santos-Mallet, J., Souza, N.A., Lins, U., Meirelles, M.N.L. and Rangel, E.F., “Ultrastructural features of the midgut epithelium of females Lutzomyia intermedia (Lutz and Nevia, 1912) (Diptera: Psychodidae: Phlebotominae)”, Mem. Inst. Oswaldo Cruz, Rio de Janeiro, 96: 1141-1151, (2001).
  • [30] Xue, L., Dallai, R. and Yin, W.-Y., “Fine structure of the midgut and hindgut in Lepidocampa weberi (Insecta, Diplura)”, Acta Zoologica (Stackholm), 71: 201-209, (1990).
  • [31] Martins, G.F., Neves, C.A., Campos, L.A.O. and Serrão, J.E., “The regenerative cells during the metamorphosis in the midgut of bees”, Micron, 37: 161-168, (2006).
  • [32] Ferreira, C., Ribeiro, A.F. and Terra, W.R., “Fine structure of the larval midgut of the fly Rhynchosciara and its physiological implications”, J. Insect. Physiol., 27: 559-570, (1981).
  • [33] Billingsley, P.F. and Downe, A.E.R., “Nondigestive cell types in the midgut epithelium of Rhodnius prolixus (Hemiptera: Reduviidae)”, J. Med. Entomol., 23: 212-216, (1986).
  • [34] Raes, H., Verbeke, M., Meulemans, W. and de Costers, W., “Organisation and ultrastructure of the regenerative crypts in the midgut of the adult worker honeybee (L. Apis mellifera)”, Tissue Cell, 26: 231-238, (1994).
  • [35] Neves, C.A., Gitirana, L.B. and Serrão, J.E., “Ultrastructure of the midgut endocrine cells in Melipona quadrifasciata anthidioides (Hymenoptera: Apidae)”, Braz. J. Biol., 63: 683- 690, (2003).
  • [36] Cruz-Landim, C., “Ultrastructural features of the regenerative cells of the bees (Hymenoptera, Apidae) midguts”, Sociobiology, 34: 597-603, (1999).
  • [37] Diaz, E., Cisneros, R., Zuniga, G. and Uria-Galicia, E., “Comparative anatomical and histological study of the alimentary canal of Dendroctonus parallelocolis, D. rhizophagus, and D. valens (Coleoptera: Scolytidae)”, Ann. Entomol. Soc. America, 91: 479–487, (1998).
  • [38] Billingsley, P.F. and Downe, A.E.R., “Ultrastructural changes in posterior midgut cells associated with blood feeding in adult female Rhodnius prolixus Stal (Hemiptera: Reduviidae)”, Can. J Zool., 61: 2574-2586, (1983).
  • [39] Anton-Erxleben, F., Miquel, J. and Philpott, D.E., “Fine-structural changes in the midgut of old Drosophila melanogaster”, Mech. Age Develop., 23: 265-276, (1983).
  • [40] Bution, M.L. and Caetano, F.H., “Ileum of the Cephalotes ants: A specialized structure to harbor symbionts microorganisms”, Micron, 39: 897-909, (2008)

Fabrius, 1793 (Hymenoptera: Sphecidae): Morphology

Year 2011, Volume: 24 Issue: 3, 397 - 405, 25.11.2011

Abstract

References

  • [1] Billen, J. and Buschinger, A., “Morphology and ultrastructure of a specialized bacterial pouch in the digestive tract of Tetraponera ants (Formicidae, Pseudomyrmecinae)”, Arthropod Structure and Development, 29: 259-266, (2000).
  • [2] Dow, J.A.T., “Insect midgut function”, Adv. Insect Physiol., 19: 187-328, (1986).
  • [3] Boonsriwong, W., Sukontason, K., Olson, J.K., Vogtsberger, R.C., Chaithong, U., Kuntalue, B., Ngern-klun, R., Upakut, S. and Sukontason, K.L., “Fine structure of the alimentary canal of the larval blow fly Chrysomya megacephala (Diptera: Calliphoridae)”, Parasitol. Res., 100: 561-574, (2007).
  • [4] Terra, W.R. and Ferrreira, C., “Insect digestive enzymes: Properties, compartmentalizasyon and function”, Com. Biochem. Physiol. B, 109: 1-62, (1994).
  • [5] Levy, S.M., Falleiros, Â.M.F., Moscardi, F., Gregório, E.A. and Toledo, L.A., “Morphological study of the hindgut in larvae of Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae)”, Neotropical Entomology, 33: 427-431, (2004).
  • [6] Uwo, M.F., Ui-Tei, K. and Takeda, P.P.M., “Replacement of midgut epithelium in the greater wax moth, Galleria mellonela, during larval-pupal moult”, Cell Tissue Res., 308: 319-331, (2002).
  • [7] Serrão, J.E., “A comparative study of the proventricular structure in corbiculate apinae (Hymenoptera, Apidae)”, Micron, 32: 379-385, (2001).
  • [8] Serrão, J.E., “Proventricular structure in the bee tribe Augochlorini (Hymenoptera: Halictidae)”, Organisms, Diversity and Evolution, 7: 175-180, (2007).
  • [9] Ameen, M. and Imam, F., “Alimentary canal of Sphaerodema rusticum (F.) (Hemiptera: Belostomatidae)”, J. Nat. History, 10: 545-568, (1976).
  • [10] Santos, C.D., Ribeiro, A.F., Ferreira, C. and Terra, W.R., “The larval midgut of cassava hornworrm (Erinnyyis ello); Ultrastructure, fluid fluxes and secretory activity in relation to the organization of digestion”, Tissue Res., 237: 565- 574, (1984).
  • [11] Anderson, E. and Harvey, W.R., “Active transport by Cecropia midgut. II. Fine structure of the midgut epithelium”, J. Cell Biol., 31: 107- 134, (1966).
  • [12] Rost, M.M., “Ultrastructural changes in the midgut epithelium in Podura aquatica L. (Insecta, Collembola, Arthropleona) during regeneration”, Arthropod Structure and Development, 35: 69-76, (2006).
  • [13] Billingsley, P.F., “The midgut ultrastructure of hematophagous insects”, Ann. Rev. Entomol., 35: 219-248, (1990).
  • [14] Garcia, J.J., Li, G., Wang, P., Zhong, J. and Granados, R.R., “Primary and continuous midgut cell cultures from Pseudaletia unipuncata (Lepidoptera, Noctuidae)”, In Vitro Cell. Develop. Biol., 37: 353-359, (2001).
  • [15] Sadrud-Din, S., Loeb, M. and Hakim, R., “In vitro differentiation of isolated stem cells from the midgut of Manduca sexta larvae”, J. Exp. Biol., 199, 319–325, (1996).
  • [16] Hakim, R.S., Baldwin, K.M. and Loeb, M., “The role of stem cells in midgut growth and regeneration”, In Vitro Cell. Develop. Biol., 37, 338–342 (2001).
  • [17] Brown, M.R., Raikhel, A.S. and Lea, A.O., “Ultrastructure of midgut endocrine cells in the adult mosquito, Aedes aegypti”, Tissue Cell, 17:709-721, (1985).
  • [18] Serrão, J.E. and Cruz-Landim, C., “Ultrastructure of midgut endocrine cells in workers of stingless bee (Hymenoptera, Apidae, Meliponinae)”. Iheringia, Série Zoologia, 81:151-156, (1996).
  • [19] Neves, C.A., Bhering, J.E., Serrão, J.E. and Gitirana, L.B., “FMRFamide-like midgut endocrine cells during the metamorphosis in Melipona quadrifasciata anthidioides Hymenoptera, Apidae)”, Micron, 33:453-460 (2002).
  • [20] Peacock, A.J., “Ultrastructure of ilrum of Locusta migratoria”, J. Morphol., 188: 191-201 (1986).
  • [21] Bution, M.L., Caetano, F.H., Britto, F.B., Tomaino Gomes, G.A. and Zara, F.J., “Histology and histochemistry of ventriculus of Dolichoderus (=Monacis) bispinosus (Oliver, 1792) (Hymenoptera: Formicidae)”, Micron: 249-254, (2006).
  • [22] Baker, J.E., Woo, S.M. and Byrd, R.V., “Ultrastructural features of the gut Sitophilus granarius (L.) (Coleoptera: Curculionidae) with notes on distribution of proteinases and amylases in crop and midgut”. Can. J. Zool., 62: 1251-1259, (1984).
  • [23] Roche, R.K. and Wheeler, D.E., “Morphological specializations of the digestive tract of Zacryptocerus rohweri (Hymenoptera: Formicidae)”, J. Morphol., 234: 253-262, (1997).
  • [24] Serrão, J.E. and Cruz-Landim, C., “Scanning electronic microscopy of proventriculus in stingless bees (Apidae: Meliponinae) with a comparision of necrophagus and feeding pollen workers”, Naturalia, 20: 207-212, (1995).
  • [25] Serrão, J.E., “A comparative study of the proventricular structure in corbiculate Apinae (Hymenoptera, Apidae)”, Micron, 32: 379-386 (2000).
  • [26] Hung, C., Lin, T. and Lee, W., “Morphology and ultrastructure of the alimentary canal of the oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae) (2): The structure of the midgut”, Zoological Studies, 39: 387-394, (2000).
  • [27] Raes, H. and Verbeke, M., “Light and electron microscopical study of two types of endocrine cell in the midgut of the adult worker honeybee (Apis mellifera)”, Tissue Cell, 26: 223-230, (1994).
  • [28] Pigino, G., Migliorini, M., Paccagnini, E., Bernini, F. and Leonzio, C., “Fine structure of the midgut Malpighian papillae in Campodea (Monocampa) quilisi Silvestri, 1932 (Hexapoda, Diplura) with special reference to the metal composition and physiological significance of midgut intracellular electron-dense granules”, Tissue Cell, 37: 223-232, (2005).
  • [29] Andrade-Coêlho, C.A., Santos-Mallet, J., Souza, N.A., Lins, U., Meirelles, M.N.L. and Rangel, E.F., “Ultrastructural features of the midgut epithelium of females Lutzomyia intermedia (Lutz and Nevia, 1912) (Diptera: Psychodidae: Phlebotominae)”, Mem. Inst. Oswaldo Cruz, Rio de Janeiro, 96: 1141-1151, (2001).
  • [30] Xue, L., Dallai, R. and Yin, W.-Y., “Fine structure of the midgut and hindgut in Lepidocampa weberi (Insecta, Diplura)”, Acta Zoologica (Stackholm), 71: 201-209, (1990).
  • [31] Martins, G.F., Neves, C.A., Campos, L.A.O. and Serrão, J.E., “The regenerative cells during the metamorphosis in the midgut of bees”, Micron, 37: 161-168, (2006).
  • [32] Ferreira, C., Ribeiro, A.F. and Terra, W.R., “Fine structure of the larval midgut of the fly Rhynchosciara and its physiological implications”, J. Insect. Physiol., 27: 559-570, (1981).
  • [33] Billingsley, P.F. and Downe, A.E.R., “Nondigestive cell types in the midgut epithelium of Rhodnius prolixus (Hemiptera: Reduviidae)”, J. Med. Entomol., 23: 212-216, (1986).
  • [34] Raes, H., Verbeke, M., Meulemans, W. and de Costers, W., “Organisation and ultrastructure of the regenerative crypts in the midgut of the adult worker honeybee (L. Apis mellifera)”, Tissue Cell, 26: 231-238, (1994).
  • [35] Neves, C.A., Gitirana, L.B. and Serrão, J.E., “Ultrastructure of the midgut endocrine cells in Melipona quadrifasciata anthidioides (Hymenoptera: Apidae)”, Braz. J. Biol., 63: 683- 690, (2003).
  • [36] Cruz-Landim, C., “Ultrastructural features of the regenerative cells of the bees (Hymenoptera, Apidae) midguts”, Sociobiology, 34: 597-603, (1999).
  • [37] Diaz, E., Cisneros, R., Zuniga, G. and Uria-Galicia, E., “Comparative anatomical and histological study of the alimentary canal of Dendroctonus parallelocolis, D. rhizophagus, and D. valens (Coleoptera: Scolytidae)”, Ann. Entomol. Soc. America, 91: 479–487, (1998).
  • [38] Billingsley, P.F. and Downe, A.E.R., “Ultrastructural changes in posterior midgut cells associated with blood feeding in adult female Rhodnius prolixus Stal (Hemiptera: Reduviidae)”, Can. J Zool., 61: 2574-2586, (1983).
  • [39] Anton-Erxleben, F., Miquel, J. and Philpott, D.E., “Fine-structural changes in the midgut of old Drosophila melanogaster”, Mech. Age Develop., 23: 265-276, (1983).
  • [40] Bution, M.L. and Caetano, F.H., “Ileum of the Cephalotes ants: A specialized structure to harbor symbionts microorganisms”, Micron, 39: 897-909, (2008)
There are 40 citations in total.

Details

Primary Language English
Journal Section Biology
Authors

Filiz Demir

Menderes Suicmez This is me

Publication Date November 25, 2011
Published in Issue Year 2011 Volume: 24 Issue: 3

Cite

APA Demir, F., & Suicmez, M. (2011). Investigation of Digestive Tract of Sphex flavipennis Fabrius, 1793 (Hymenoptera: Sphecidae): Morphology and Ultrastructure. Gazi University Journal of Science, 24(3), 397-405.
AMA Demir F, Suicmez M. Investigation of Digestive Tract of Sphex flavipennis Fabrius, 1793 (Hymenoptera: Sphecidae): Morphology and Ultrastructure. Gazi University Journal of Science. November 2011;24(3):397-405.
Chicago Demir, Filiz, and Menderes Suicmez. “Investigation of Digestive Tract of Sphex Flavipennis Fabrius, 1793 (Hymenoptera: Sphecidae): Morphology and Ultrastructure”. Gazi University Journal of Science 24, no. 3 (November 2011): 397-405.
EndNote Demir F, Suicmez M (November 1, 2011) Investigation of Digestive Tract of Sphex flavipennis Fabrius, 1793 (Hymenoptera: Sphecidae): Morphology and Ultrastructure. Gazi University Journal of Science 24 3 397–405.
IEEE F. Demir and M. Suicmez, “Investigation of Digestive Tract of Sphex flavipennis Fabrius, 1793 (Hymenoptera: Sphecidae): Morphology and Ultrastructure”, Gazi University Journal of Science, vol. 24, no. 3, pp. 397–405, 2011.
ISNAD Demir, Filiz - Suicmez, Menderes. “Investigation of Digestive Tract of Sphex Flavipennis Fabrius, 1793 (Hymenoptera: Sphecidae): Morphology and Ultrastructure”. Gazi University Journal of Science 24/3 (November 2011), 397-405.
JAMA Demir F, Suicmez M. Investigation of Digestive Tract of Sphex flavipennis Fabrius, 1793 (Hymenoptera: Sphecidae): Morphology and Ultrastructure. Gazi University Journal of Science. 2011;24:397–405.
MLA Demir, Filiz and Menderes Suicmez. “Investigation of Digestive Tract of Sphex Flavipennis Fabrius, 1793 (Hymenoptera: Sphecidae): Morphology and Ultrastructure”. Gazi University Journal of Science, vol. 24, no. 3, 2011, pp. 397-05.
Vancouver Demir F, Suicmez M. Investigation of Digestive Tract of Sphex flavipennis Fabrius, 1793 (Hymenoptera: Sphecidae): Morphology and Ultrastructure. Gazi University Journal of Science. 2011;24(3):397-405.