The Effect of Nanostructured Titanium Surface on Protein Adsorption

Abstract

The amount and conformation of bovine serum albumin upon adsorption on titanium (Ti) surfaces containing nanotubes with different pore sizes were investigated. Nanotubes were created on the surfaces via anodization. Protein adsorption behavior on anodized surfaces were compared with the adsorption behavior on smooth and sanded Ti surfaces. The conformational changes in surface adsorbed proteins were evaluated using the second derivative and curve fitting methods applied to the Fourier transform infrared spectra of the surfaces. Results showed that the amount of protein adsorbed on the surfaces increased significantly with increasing surface roughness and a significant change in the conformation of the adsorbed protein occurred on every surface albeit in a different fashion. When anodized samples were considered, it was observed that the changes in the secondary structure seemed to be correlated with to the pore size of the nanotubes rather than the surface roughness.

Keywords

• Titanium Implant
• Nanotube
• Anodic Oxidation Protein
• Protein Adsorption

Supporting Institution

Ankara Hacı Bayram Veli University Scientific Research Projects Coordination Centre

Project Number

(Project No. 01/2020-24

Thanks

SEM imaging have been performed at Hacettepe University, Advanced Technologies Research and Applications Center (HUNITEK). AFM imaging was performed at National Nanotechnology Research Center Institute of Materials Science and Nanotechnology (UNAM). FTIR-ATR analyses were performed at Eastern Anatolia High Technology Application and Research Center (DAYTAM).

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