Synthesis and Characterisation of a New Hydrophilic Interaction/Reversed Phase Mixed-Mode Chromatographic Stationary Phase

Yıl 2018, Cilt: 46 Sayı: 1, 43 - 52, 01.03.2018

Hayriye Aral

Öz

A novel multifunctional stationary phase based the amide-silica was synthesised starting from L-isoleucine
and 2-aminoacetanilide. Hereby, compounds 1, 2, 3 and 4 were synthesised for the first time and 1, 2 and 4
were identified. The stationary phase was synthesised by a reliable and repeatable method and characterized
by elemental analysis, solid state 13C NMR, scanning electron microscope (SEM), and Brunauer, Emmett and
Teller (BET). This stationary phase possess four amide groups as polar cites, phenyl ring as aromatic non-polar
cite and isopropyl as an aliphatic non-polar cite. Therefore, it can act as both hydrophilic interaction (HILIC)
and reversed phase (RPLC) chromatographic stationary phase. The stationary phase has also chirality and can
be used in enantioseparation of racemic compounds in normal phase chromatography (NPLC).

Anahtar Kelimeler

Mixed-mode stationary phase, Hydrophilic interaction liquid chromatography (HILIC), Isoleucine, Chiral stationary phase

Kaynakça

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