The In Vitro Impacts of Some Plant Extracts on Carbonic Anhydrase I, II and Paraoxonase-1

Yıl 2019, Cilt: 47 Sayı: 1, 51 - 59, 01.02.2019

Elif Duygu Kaya Bülent Erğun Yeliz Demir Zuhal Alım Şükrü Beydemir

Öz

The presented article focuses on the in vitro inhibition of plant extracts on the human carbonic anhydrase isoforms (hCA I and hCAII), and paraoxonase-1 (PON1) activities. Five different plants (Alcea rosea, Foeniculum vulgare, Elettaria cardamomum, Laurus azorica and Lavandula stoechas) were selected in this study. Methanol, ethanol, and water extracts of plants were prepared and the concentration-dependent inhibition degrees were found for hCA I and hCA II isozymes and hPON1 spectrophotometrically. Thus, IC50 (mg/mL) values were obtained for each extract. Methanolic extract of Elettaria cardamomum has the highest inhibitory effects (0.032 mg/mL). The water extracts of plants showed lower inhibitory impacts compared to the methanol and ethanol extracts.

Anahtar Kelimeler

Carbonic anhydrase, inhibition, paraoxonase, plant extract

Kaynakça

• C.H. Chan, G.C. Ngoh, R. Yusoff, A brief review on anti diabetic plants: global distribution, active ingredients, extraction techniques and acting mechanisms, Pharmacogn. Rev., 6 (2012) 22-28.
• 2. N.J. Sucher, M..C. Carles, A pharmacological basis of herbal medicines for epilepsy, Epilepsy Behav., 52 (2015) 308-318.
• 3. P.R. Bhandari, A comment on effect of plant extracts on alzheimer’s disease: an insight into therapeutic avenues, J. Neurosci. Rural Pract., 4 (2013) 236-237.
• 4. H.R. Vasanthi, N. Shrishrimal, D.K. Das, Phytochemicals from plants to combat cardiovascular disease, Curr. Med. Chem., 19 (2012) 2242-2251.
• 5. D. Krishnaiah, R. Sarbatly, R.Nithyanandam, A review of the antioxidant potential of medicinal plant species, Food Bioprod. Process, 89 (2011) 217-233.
• 6. A.E. Al-Snafi. The Pharmaceutical Importance of althaea officinalis and Althaea rosea: a review, Int. J. Pharm. Tech. Res., 5 (2013) 1385-1387.
• 7. P. Sadighara, S. Gharibi, A.M. Jafari, G.J. Khaniki, S. Salari, The antioxidant and flavonoids contents of althaea officinalis l. flowers based on their color, Avicenna J. Phytomed., 2 (2012) 113-117.
• 8. M. Kwiatkowska, D. Stepinski, K. Poplonska, A. Wojtczak, TJ. Polit, ‘Elaioplasts’ identified as lipotubuloids in Althaea rosea, funkia sieboldiana and vanilla planifolia contain lipid bodies connected with microtubules, Acta Soc. Bot. Pol., 80 (2011) 211-219.
• 9. R. Piccaglia, M. Marotti, Characterization of some Italian types of wild fennel (Foeniculum Vulgare Mill.), J. Agric. Food Chem., 49 (2001) 239-44.
• 10. S.B. Badgujar, V.V. Patel, A.H. Bandivdekar, Foeniculum Vulgare Mill: A review of its botany, phytochemistry, pharmacology, contemporary application, and toxicology, Biomed. Res. Int., (2014) Article ID 842674.
• 11. M.E. Leblebici, S. Machmudah, M. Sasaki, M. Goto, Antiradical efficiency of essential oils from plant seeds obtained by supercritical CO2, soxhlet extraction, and hydrodistillation, Sep. Sci. Technol., 48 (2012) 328-337.
• 12. H. Al-Zuhair, B. El-Sayeh, HA. Ameen, H. Al-Shoora. Pharmacological studies of cardamom oil in animals, Pharmacol. Res., 34, (1996) 79-82.
• 13. I. Kubo, M. Himejima, H. Muroi, Antimicrobial activity of flavor components of cardamom Elettaria cardamomum (zingiberaceae) seed, J. Agric Food Chem, 39, (1991) 1984- 1986.
• 14. O. Ouchikh, T. Chahed, R. Ksouri, M.B.Taarit, H. Faleh, C. Abdelly, M.E. Kchouk, B. Marzouk, The effects of extraction method on the measured tocopherol level and antioxidant activity of L. nobilis vegetative organs, J. Food Comp. Anal., 24 (2011) 103-110.
• 15. C. Da Porto, D. Decorti, Analysis of the volatile compounds of f lowers a nd e ssential o ils f rom Lavandula angustifolia cultivated in northeastern italy by headspace solid-phase microextraction coupled to gas chromatography-mass spectrometry, Planta Med., 74 (2008) 182-187.
• 16. A. Goren, G. Topçu, G. Bilsel, M. Bilsel, Z. Aydoğmuş, J.M. Pezzuto, The chemical constituents and biological activity of essential oil of Lavandula stoechas ssp. Stoechas, Z. Naturforsch C, 57 (2002) 797-800.
• 17. T. Benabdelkader, A. Zitouni, Y. Guitton, F. Jullien, D. Maitre, H. Casabianca, L. Legendre, A. Kameli, Essential oils from wild populations of algerian Lavandula stoechas l.: composition, chemical variability, and in vitro biological properties, Chem. Biodivers., 8 (2011) 937-953.
• 18. D. Kaya, MI. Alpaslan, E.S. Giray, S. Kirici, Diurnal, Ontogenetic and morphogenetic variability of Lavandula stoechas L. Ssp. Stoechas in East Mediterranean region, Rev. Chim. (Bucharest Rom), 63 (2012) 749-753.
• 19. M. Zuzarte, Gonçalves M.J, Cavaleiro C, Cruz M.T, Benzarti A, Marongiu B, Maxia A, Piras A, Salgueiro L. Antifungal and anti-inflammatory potential of Lavandula stoechas and thymus herba-barona essential oils, Ind. Crops Prod., 44 (2013) 97-103.
• 20. H.A. Aslan, Y. Demir, M.S. Özaslan, F. Türkan, Ş. Beydemir, O.I. Kufrevioglu, The Behaviour of some chalcones on acetycholinesterase and carbonic anhydrase activity, Drug Chem. Toxicol., 4 (2018) 1-7.
• 21. S. Mert, Z. Alim, MM. Isgor, S. Beydemir, R. Kasimogullari, The synthesis of novel pyrazole-3,4-dicarboxamides bearing 5-amino-1,3,4-thiadiazole-2-sulfonamide moiety with effective inhibitory activity against the isoforms of human cytosolic carbonic anhydrase I and II, Bioorg. Chem., 68, (2016) 64-71.
• 22. A. Topal, M. Atamanalp, E. Oruc, Y. Demir, S. Beydemir, A. Isik, In vivo changes in carbonic anhydrase activity and histopathology of gill and liver tissues after acute exposure to chlorpyrifos in rainbow trout, Arh. Hig. Rada. Toksikol., 65, (2014) 377-385.
• 23. Y. Demir, E. Oruç, A. Topal. Carbonic anhydrase activity responses and histopathological changes in gill and liver tissues after acute exposure to chromium in brown trout juveniles, Hacettepe J. Biol. & Chem., 44 (2016) 515-523.
• 24. A. Innocenti, I. Gulcin, A. Scozzafava, C.T. Supuran, Carbonic anhydrase inhibitors. antioxidant polyphenols effectively inhibit mammalian isoforms I-XV, Bioorg. Med. Chem. Lett., 20 (2010) 5050-5053.
• 25. H. Balseven, M.M. İşgor, S. Mert, Z. Alim, Ş. Beydemir, S. Ok, R. Kasimogullari, Facile synthesis and characterization of novel pyrazole-sulfonamides and their inhibition effects on human carbonic anhydrase isoenzymes, Bioorg. Med. Chem., 21 (2013) 21-27.
• 26. Z. Alim, N. Kilinc, M.M. Isgor, B. Sengul, S. Beydemir, Some anti-inflammatory agents inhibit esterase activities of human carbonic anhydrase isoforms I and II: an in vitro study, Chem. Biol. Drug. Des., 86 (2015) 857-863.
• 27.H.A. Alici, D. Ekinci, Ş. Beydemir, Intravenous anesthetics inhibit human paraoxonase-1 (PON1) activity in vitro and in vivo, Clin. Biochem., 41 (2008) 1384-1390.
• 28. S. Beydemir, Y. Demir, Antiepileptic drugs: Impacts on human serum paraoxonase-1, J. Biochem. Mol. Toxicol., 31 (2017) 1-6 .
• 29. Y. Demir, S. Beydemir, Purification, refolding, and characterization of recombinant human paraoxonase-1, Turk J. Chem., 39 (2015) 764-776.
• 30. M. Oktay, I. Gülçin, O.I. Küfrevioğlu, Determination of in vitro antioxidant activity of fennel (Foeniculum vulgare) seed extracts, LWT- Food Sci. Technol. 36 (2003) 263-271.
• 31. E.D. Kaya, H. Soyut, Ş. Beydemir, The toxicological impacts of some heavy metals on carbonic anhydrase from gilthead sea bream (Sparus aurata) gills, Environ. Toxicol. Pharmacol., 39 (2015) 825-832.
• 32. D. Ekinci, Ş. Beydemir, Z. Alim, Some drugs inhibit in vitro hydratase and esterase activities of human carbonic anhydrase-I and II, Pharmacol. Rep., 59 (2007) 580-587.
• 33. Z. Alim, B. Çamur, Ş. Beydemir, O.I. Küfrevioglu, The correlation between some metal concentrations and carbonic anhydrase activity in tuna (thunnus thynnus linnaeus, 1758) gill, Hacettepe J. Biol. & Chem., 42 (2014) 219 – 224.
• 34. J.A. Verpoorte, S. Mehta, J.T. Edsall, Esterase activities of human carbonic anhydrases B and C, J. Biol. Chem., 242 (1967) 4221-4229.
• 35. M.M. Bradford, Rapid and sensitive method for quantitation of microgram quantities of protein utilizing principle of protein-dye binding, Anal. Biochem., 72 (1976) 248-254.
• 36. Y. Demir, M. Isik, I. Gulcin, Ş. Beydemir, Phenolic compounds inhibit the aldose reductase enzyme from the sheep kidney, J. Biochem. Mol. Toxicol., 31 (2017) e21935.
• 37. Y. Demir, M. Şenol Kotan, N. Dikbaş, Ş. Beydemir, Phytase from Weissella halotolerans: purification, partial characterisation and the effect of some metals, Int. J. Food Prop., 20 (2017) 2127-2137.
• 38. M.S. Ozaslan, Y. Demir, O.I. Kufrevioglu, M. Ciftci, Some metals inhibit the glutathione S-transferase from Van Lake fish gills, J. Biochem. Mol. Toxicol., 31 (2017) e21967.
• 39. U.K. Laemmli, Cleavage of structural proteins during assembly of head of bacteriophage-T4, Nature, 227 (1970) 680–683.
• 40. M. Kırıcı, M. Kırıcı, Y. Demir, Ş. Beydemir, M. Atamanalp, The effect of Al3+ a nd H g2+ on glucose 6-phosphate dehydrogenase from capoetaumbla kidney, Appl. Ecol. Env. Res., 14 (2016) 253-264
• 41. Y. Demir, B. Şengül, B. Ergun, Ş. Beydemir, Alcohol dehydrogenase from sheep liver: Purification, characterization and impacts of some antibiotics, Iğdır Univ. J. Inst. Sci. & Tech., 7 (2017) 151-159.
• 42. M. Isik, Y. Demir, M. Kirici, R. Demir, F. Simsek, Ş. Beydemir, Changes in the anti-oxidant system in adult epilepsy patients receiving anti-epileptic drugs, Arch. Physiol. Biochem., 121 (2015) 97-102.
• 43. H. Sahin, Z. Can, O. Yildiz, S. Kolayli, A.G. Innocenti, Scozzafava, C.T. Supuran, Inhibition of carbonic anhydrase isozymes i and ii with natural products extracted from plants, mushrooms and honey, J. Enzyme. Inhib. Med. Chem., 27 (2012) 395-402.
• 44. M.F. Asaolu, Chemical compositions and phytochemical screening of the seeds of garcinia kola (bitter kola), Pak. J. Sci. Ind. Res., 46 (2003) 145-147.
• 45. M. Munir, A. Hussain, I. Ul-Haq, R. Qureshi, M. Munazir, M. Rshad, M. Khan, Callogenesis potential of cotyledonary explants of Althaea rosea from Pakistan, Pak. J. Bot., 44 (2012) 271-275.
• 46. T. Mert, T. Erdogan, B. Kivcak, T. Ozturk, Antimicrobial and cytotoxic activities of the extracts obtained from the flowers of Alcea rosea L., J, HUJPHARM, 30 (2010) 17-24.
• 47. F. Namjoyan, A. Jahangiri, M.E. Azemi, E. Arkian, H. Mousavi, Inhibitory effects of physalis alkekengi l., Alcea rosea l., Bunium persicum b. fedtsch. and Marrubium vulgare l. on mushroom tyrosinase, Jundishapur. J. Nat. Pharm. Prod., 20 (2015) 23356.
• 48. M. Faudale, F. Viladomat, J. Bastida, F. Poli, C. Codina, Antioxidant activity and phenolic composition of wild, edible, and medicinal fennel from different mediterranean countries, J. Agric. Food Chem., 56 (2008) 1912-1920.
• 49. S.F. Subehan Zaidi, S. Kadota, Y. Tezuka, Inhibition on human liver cytochrome p450 3a4 by constituents of fennel (Foeniculum vulgare): identification and characterization of a mechanism-based inactivator, J. Agric. Food Chem., 55 (2007) 10162-67.
• 50. B. Marongiu, A. Piras, S. Porcedda, Comparative analysis of the oil and supercritical CO2, extract of Elettaria Cardamomum (L.) Maton, J. Agric. Food Chem., 52 (2004) 6278-682.
• 51. A. Carrasco, O.R. Vanessa, M.G. Ramiro, T. Virginia, J. Tudela Lavandula stoechas essential oil from spain: aromatic profile determined by gas chromatography–mass spectrometry, antioxidant and lipoxygenase inhibitory bioactivities, Ind. Crops Prod., 73 (2015) 16-27.