Antibiofilm Activity and Chemical Contents of Propolis Samples From Manisa-Turkey

Yıl 2016, Cilt: 44 Sayı: 4, 505 - 513, 01.11.2016

Gülümser Acar Doğanlı Gülçin Ülger Nazime Mercan Doğan

Öz

In this study, the inhibition of biofilm formation and the reduction of preformed or established biofilm by etha- nol extract of propolis samples EEP obtained from Manisa-Turkey was investigated and their chemical com- position was screened. The antibiofilm effect of the propolis extracts against biofilm forming bacteria Listeria monocytogenes ATCC 7644, Methicillin Sensitive Staphylococcus aureus clinical isolate MSSA M20, S. aureus ATCC 33862, S. aureus ATCC 29213, Enterococcus faecalis ATCC 19433, Pseudomonas fluorescens ATCC 55241, Micrococcus luteus NRRL-B1013 was tested on 96-well polystyrene plates using crystal violet assay. Also, the antibacterial activity of EEP was evaluated according to Agar Well Diffusion method. Chemical composition of extracts was detected by Gas Chromatography-Mass Spectrometry GC/MSD analyze. The EEP samples exhi- bited good antibiofilm activity against bacteria. The maximum biofilm inhibition activity percentage of MP-1 Manisa-Köprübaşı , MP-2 Manisa-Demirci and MP-3 Manisa-Kula were found as 89.4%, 80.0% and 89.0% for L. monocytogenes ATCC 7644 and 66.0%, 67.0% and 74.0% for MSSA M20, respectively. According to GC/ MSD analyze, triacontyl acetate was the major compound found in propolis extracts.

Anahtar Kelimeler

Antibiofilm, Biofilm reduction, Propolis, GC/MSD

Türkiye-Manisa’dan Propolis Örneklerinin Kimyasal İçeriği ve Antibiyofilm Aktivitesi

Öz

B u çalışmada, Manisa’dan elde edilen propolis örneklerinin etanol özütlerinin EEP biyofilm oluşumunun inhibisyonu ve oluşmuş biyofilm tabakasının giderimi üzerine etkileri ile kimyasal kompozisyonları araştırılmıştır. Propolis özütlerinin antibiyofilm etkisi biyofilm oluşturan bakterilere karşı Listeria monocytogenes ATCC 7644, Methicillin Sensitive Staphylococcus aureus clinical isolate MSSA M20, S. aureus ATCC 33862, S. aureus ATCC 29213, Enterococcus faecalis ATCC 19433, Pseudomonas fluorescens ATCC 55241, Micrococcus luteus NRRL-B1013 96 kuyucuklu polisitiren plakalarda kristal viole yöntemi kullanılarak test edilmiştir. Ayrıca, EEP’nin antibakteriyel aktivitesi Agar Kuyu Difüzyon yöntemine göre belirlenmiştir. Özütlerin kimyasal kompozisyonu Gaz Kromatografisi-Kütle Spektrometresi analizi GC/MSD ile tespit edilmiştir. EEP örnekleri bakterilere karşı iyi antibiyofilm aktivite sergilemişlerdir. MP-1 Manisa-Köprübaşı , MP-2 ManisaDemirci ve MP-3 Manisa-Kula ’ün maksimum biyofilm inhibisyon aktivitesi oranları L. monocytogenes ATCC 7644 için sırasıyla %89.4; %80.0 ve %89.0 olarak, MSSA M20 için de sırasıyla %66.0; %67.0 ve %74.0 olarak bulunmuştur. GC/MSD analiz sonucuna göre triakotinil asetat propolis özütlerinde bulunan başlıca bileşiktir

Anahtar Kelimeler

Antibiyofilm, Biyofilm indirgeme, Propolis, GC/MSD.

Kaynakça

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