Design and Characterization of a Green-Synthesized Magnetic Hybrid 1 Material for Local Antibiotic Delivery in Periodontal Therapy

Abstract

Bu çalışma, periodontitis tedavisinde Tetrasiklin HCl'nin lokal iletimi için manyetik bir nanotaşıyıcı sistem geliştirmeye odaklanmaktadır. pH-15'e duyarlı özelliklere sahip manyetik nanopartiküller, hem kaplama hem de indirgeyici ajan olarak Camellia sinensis (yeşil çay) kullanılarak çevre dostu bir yöntemle sentezlendi. FTIR, SEM ve Mössbauer spektroskopisi yöntemleri kullanılarak yapılan karakterizasyon çalışması, hibrit biyokompozitin başarılı bir şekilde sentezlendiğini ve ilacı taşımada etkili olduğunu ortaya koydu. Manyetik C. sinensis nanopartikülleri (MCs NPs), %95,43'lük olağanüstü bir ilaç yükleme kapasitesiyle 41,157 m2/g'lık yüksek bir yüzey alanına sahiptir. İn vitro salım çalışmaları, ilacın fizyolojik pH'da (7,4) gün boyunca kontrollü ve sürekli bir şekilde salınabileceğini gösterdi. Enfekte periodontal bölgeyi temsil eden pH 6,25'te, MC'ler yaklaşık 8,6 saat içinde hızlı, pH-24'e duyarlı bir terapötik salınım profili sergiledi. Bu bulgular, MC sisteminin periodontitisin lokalize ve etkili tedavisi için umut vadeden bir aday olduğunu göstermektedir.

Keywords

• Yeşil sentez
• Camellia sinensis
• Manyetik nanopartiküller
• Periodontal ilaç dağıtımı
• pH'a duyarlı.

Supporting Institution

TÜBİTAK

Project Number

TÜBİTAK 2209-A Undergraduate Research Projects Support Program-Project No: 1919B012306483

Ethical Statement

Bu çalışma insan katılımcı veya hayvan içermediği için etik onay gerekmemiştir.

Thanks

Bu çalışma, Türkiye Bilim ve Teknolojik Araştırma Konseyi (TÜBİTAK) tarafından yürütülen TÜBİTAK 2209-A Lisans Araştırma Projeleri Destek Programı kapsamında, 1919B012306483 proje numarasıyla desteklenmiştir. Yazarlar, bu araştırmayı yürütme fırsatı sağladığı için TÜBİTAK'a minnettardır. Yazarlar ayrıca, bu çalışmada gerçekleştirilen Mössbauer analizine ilişkin değerli yardımları ve uzmanlığı için Hitit Üniversitesi'nden Prof. Dr. Hakan GÜNGÜNEŞ'e içten teşekkürlerini sunarlar.

Design and Characterization of a Green-Synthesized Magnetic Hybrid Material for Local Antibiotic Delivery in Periodontal Therapy

Abstract

This study focuses on developing a magnetic nanocarrier system for local delivery 14 of Tetracycline HCl to treat periodontitis. Magnetic nanoparticles with pH-15 responsive properties were synthesized using an environmentally friendly method 16 with Camellia sinensis (green tea) as both a capping and reducing agent. The 17 characterization study using FTIR, SEM, and Mössbauer spectroscopy methods 18 revealed that the hybrid biocomposite was successfully synthesized and effective at 19 carrying the drug. The Magnetic C. sinensis nanoparticles (MCs NPs) have a high 20 surface area of 41.157 m2/g with an exceptional drug loading capacity of 95.43%. 21 In vitro release studies showed that the drug could be released in a controlled and 22 continuous manner for 23 days at physiological pH (7.4). At pH 6,25, which 23 represents the infected periodontal region, the MCs demonstrated a rapid, pH-24 responsive therapeutic release profile in nearly 8,6 hours. These findings suggest 25 that the MCs system is a promising candidate for localized and effective treatment 26 of periodontitis.

Keywords

• Green synthesis
• Camellia sinensis
• Magnetic nanoparticles
• 29 Tetracycline HCl
• Periodontal drug delivery
• pH-responsive

Supporting Institution

TUBITAK

Project Number

TÜBİTAK 2209-A Undergraduate Research Projects Support Program-Project No: 1919B012306483

Ethical Statement

Ethical approval was not required for this study as it did not involve human participants or animals.

Thanks

This study was supported by the TÜBİTAK 2209-A Undergraduate Research 468 Projects Support Program carried out by The Scientific and Technological Research Council of Türkiye (TÜBİTAK) under Project Number: 1919B012306483. The authors are grateful to TÜBİTAK for providing the opportunity to conduct this research. The authors also express their sincere gratitude to Professor Dr. Hakan GÜNGÜNEŞ of Hitit University for his invaluable assistance and expertise regarding the Mössbauer analysis conducted in this study.

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