Probiotic Properties of Some Lactobacillus spp. That Can Survive in the Presence of Viral Gastroenteritis

Year 2019, , 261 - 267, 31.12.2019

Elmas Ceren Ikiz Gulcin Avci Hande Alp

https://doi.org/10.17350/HJSE19030000156

Abstract

Viral acute gastroenteritis is a serious health problem worldwide with the high morbidity and mortality rates. In fact there is no available antiviral treatment for gastroenteritis alternatively, the use of probiotics on which have numerous benefits, has been on the increase recently. Therefore, to be able to identify the strains that can survive in viral acute gastroenteritis and their distinguishable features, seven strains of Lactobacillus spp. were isolated from the stool samples of 0-5 year old children with viral gastroenteritis in this study. Strains were identified by API 50 CH test. EPS production capacities, acid resistance, bile tolerance, antibiotic susceptibilities, and antimicrobial activities against Escherichia coli ATCC 25922 were determined in the order to investigate their probiotic features. Strains were characterized as Lactobacillus plantarum. All strains survived in De Man Rogosa and Sharpe MRS broth with adjusted pH values of 2 and 3, despite high inhibition rates 95.2-99.2% and 98.3-99.2%, respectively . Furthermore, all strains maintained their viability within MRS broth mediums that contain 0.15%, 0.2% and 0.3% bile viability rates as 81.4-92.5%, 80.9- 87.3% and 73.2-89.2%, respectively . Exopolysaccharide production 4.13-50.33 mg/mL was observed in all strains except for 182a. No antimicrobial activity was detected against E. coli ATCC 25922. All strains experienced high sensitivity to erythromycin while showing resistance to vancomycin. In conclusion, L. plantarum strains obtained in this study can be further investigated for describe other probiotic features and may be used for the production of new probiotic products to provide sufficient therapies in further studies.

Keywords

Acute Gastroenteritis, Probiotics, Lactobacillus spp.

References

• Collins MD, Ribson GR. Probiotics, prebiotics and Synbiotics: Approaches for modulating the microbial ecology of the gut. American Journal of Clinical Nutrition 69 (1999) 1052-1057.
• Arrieta MC, Stiemsma LT, Amenyogbe N, Brown EM, Finlay B. The intestinal microbiome in early life: health and disease. Frontiers in Immunology 5 (2014) 427.
• Annuk H, Shchepetova J, Kullisaar T, Songisepp E, Zilmer M, Mikelsaar M. Characterization of intestinal lactobacilli as putative probiotic candidates. Journal of Applied Microbiology 94 (2003) 403-412.
• Sabir F, Beyatli Y, Cokmus C, Onal-Darilmaz D. Assessment of potential probiotic properties of Lactobacillus spp., Lactococcus spp., and Pedicoccus spp. Strains isolated from Kefir. Journal of Food Science 75 (2010)
• Shokryazdan P, Sieo CC, Kalavathy R, Liang JB, Alitheen NB, Faseleh Jahromi M, Ho YW. Probiotic Potential of Lactobacillus Strains with Antimicrobial Activity against Some Human Pathogenic Strains. BioMed Research International (2014) 927268.
• Verdenelli MC, Ghelfi F, Silvi S, Orpianesi C, Cecchini C, Cresci A. Probiotic properties of Lactobacillus rhamnosus and Lactobacillus paracasei isolated from human faeces. European Journal of Nutrition 48 (2009) 355-363.
• Nawaz M, Wang J, Zhou A, Ma C, Wu X, Xu J. Screening and characterization o new potentially probiotic lactobacilli from breast-fed healthy babies in Pakistan. African Journal of Microbiology Research 5 (2011) 1428-1436.
• Papamanoli E, Tzanetakis N, Litopoulou-Tzanetaki E, Kotzekidou P. Characterization of lactic acid bacteria isolated from a Greek dry- fermented sausage in respect of their technological and probiotic properties. Meat Science (2003) 859–867.
• Kotsou MG, Mitsou EK, Oikonomou IG, Kyriacou AA. In vitro assessment of probiotic properties of Lactobacillus strains from infant gut microflora. Food Biotechnology 22 (2008) 1-17.
• Nikolic M, Lopez P, Strahinic I, Suarez A, Kojic M, Fernandez-Garcia M, Topisirovic L, Golic N, Ruas- Madiedo P. Characterization of the exopolysaccharide (EPS)- producing Lactobacillus paraplantarum BGCG11 and its non-EPS producing derivative strains as potential probiotics. International Journal of Food Microbiology 158 (2012) 155-162.
• Caggianiello G, Kleerebezem M, Spano G. Exopolysaccharides produced by lactic acid bacteria: from health-promoting benefits to stress tolerance mechanisms. Applied Microbiology Biotechnology 100 (2016) 3877-86.
• Aslim B, Yüksekdağ ZN, Beyatli Y, Mercan N. Exopolysaccharide production by Lactobacillus delbruckii subsp. bulgaricus and Streptococcus thermophilus strains under different growth conditions. World Journal of Microbiology & Biotechnology 21 (2005) 673–677.
• Ren D, Li C, Qin Y, Yin R, Du S, Ye F, Liu C, Liu H, Wang M, Li Y, Sun Y, Li X, Tian M, Jin N. In vitro evaluation of the probiotic and functional potential of Lactobacillus strains isolated from fermented food and human intestine. Anaerobe 30 (2014) 1-10.
• EUCAST Disk Diffusion Method for Antimicrobial Susceptibility Testing - Version 7.0 (January 2019) http://www.eucast. org/fileadmin/src/media/PDFs/EUCAST_files/Disk _test_ documents/2019_manuals/Manual_v_7.0_EUCAST_Disk_ Test_2019.pdf
• Temmerman R, Pot B, Huys G, Swings J. Identification and Antibiotic Susceptibility of Bacterial Isolates from Probiotic Products. International Journal of Food Microbiology 81 (2003) 1-10.
• Kirtzalidou E, Pramateftaki P, Kotsou M, Kyriacou A. Screening for lactobacilli with probiotic properties in the infant gut microbiota. Anaerobe 17 (2011) 440-443.
• Klopper KB, Deane SM, Dicks LMT. Aciduric Strains of Lactobacillus reuteri and Lactobacillus rhamnosus, Isolated from Human Feces, Have Strong Adhesion and Aggregation Properties. Probiotics and Antimicrobial Proteins 10 (2017) 89-97.
• Olivares M, Diaz-Ropeo MP, Martin R, Rodriguez JM, Xausi J. Antimicrobial potential of four Lactobacillus strains isolated from breast milk. Journal of Applied Microbiology 101 (2006) 72-9.