In this study, nanofibrous biohybrid scaffolds were developed by electrospinning using poly(ε-caprolactone) (PCL) and decellularized bovine tissue derived extracellular matrix (ECM). At the first part of the study, bovine ECM was decellularized by treatment with detergent for 24h and then combined with PCL. Following the evaluation of the decellularization efficiency via spectrophotometric DNA content analysis, the composite scaffolds were characterized by using SEM and FT-IR spectroscopy. Moreover, to assess the biocompatibility of the scaffolds an in vitro cell culture based cytotoxicity test was performed. The results indicated that, DNA content of the bovine tissue was reduced by ~80% compared to the native tissue after decellularization. While FT-IR results indicated the presence of ECM in the composite scaffolds, SEM findings showed that the porous nanofibrous structure of the scaffold changed depending on the incorporated ECM amount. Cell culture based studies also revealed that, the scaffolds containing different amounts of ECM did not have any toxic effect on cell viability during 48 hours of culture period.
biomaterials tissue engineering decellularization electrospinning
Birincil Dil | İngilizce |
---|---|
Konular | Mühendislik |
Bölüm | Research Article |
Yazarlar | |
Yayımlanma Tarihi | 30 Haziran 2021 |
Gönderilme Tarihi | 11 Mart 2021 |
Yayımlandığı Sayı | Yıl 2021 |
Hittite Journal of Science and Engineering Creative Commons Atıf-GayriTicari 4.0 Uluslararası Lisansı (CC BY NC) ile lisanslanmıştır.