Effect of Fibroblast Growth Factor (FGF) on Some Serum Oxidative Parameters in Hyperglycemic Rats

Abstract

Wound healing is a complex and dynamic process that includes multiple biological pathways and has some successive healing periods. Most growth factor is responsible for wound healing. Fibroblast growth factor has a positive effect on wound healing problems that can be caused by diabetes. In the present study, we aimed to investigate exogenous effect bFGF supplementation on serum TBARS, RSH and NOx levels in hyperglysemic rats. Experiments were performed on 30 male Wistar albino rats (weight range: 200-250 g ). Rats were hyperglycemic with STZ (ip, 60 mg/kg). The experimental groups were divided into untreated and bFGF-treated subgroups. bGF was applied locally to the dorsalateral wounds of rats (10 ng/ml). After these administrations, on the 3th and 7th days of wound healing, the animals were sacrificed. Serum TBARS, RSH and NOx levels were recorded spectrophotometrically. The results were expressed as mean ± Standard deviation and the mean differences were compared by Anova Variance Analysis (p<0,05). When compared with the treatment group, on the 7th day and the 3rd day, it was found that the serum TBARS levels increased statistically in hyperglycemic rats(p<0,05). Both in the 3rd day of the untreated and 3rd day of the rats treated with bFGF may significant decrease in the serum RSH levels. bFGF application was found both enhancing and reducing effects on oxidative stres. In subsequent studies, the effect of bFGF, which has positive effects on diabetic wound healing, on oxidative events can be investigated in detail using different doses and different treatment periods.

Keywords

• bFGF
• diabetic wound healing
• free radicals
• oxidative stress
• diabetes mellitus

References

1. Reference1. Yüceyar, S. (2008). Cerrahi Yaraların Takip ve Tedavisi, İ. Ü. Cerrahpaşa Tıp Fakültesi Sürekli Tıp Eğitimi Etkinlikleri Sempozyum Dizisi, İstanbul. 67, 17-24.
2. Reference2. Gerald, M.C., Diane, R.K., David, J.M., David, E.P., Roger, R. and Martin, C. (1994). Definitions and guidelines for assessment of wounds and evaluation of healing. Wound Repair and Regeneration, 2(2), 165–170.
3. Reference3. Barrientos, S., Brem, H., Canic, M.C., Golinko., M.S. and Stojadinovic, O. (2008). Growth factors and cytokines in wound healing. Wound Repair and Regeneration, 16(5), 585–601.
4. Reference4. Karasu, A., Bakır, B. (2008). Yara ve Yara İyileşmesi. Veteriner Cerrahi Dergisi, 14(1), 36-43.
5. Reference5. Taşır, S. (2007). Ekzojen epidermal büyüme faktörü’nün tükürük bezi oksidan olaylarına zaman bağımlı etkisi, Yüksek Lisans Tezi, Gazi Üniversitesi Fen Bilimleri Enstitüsü, Ankara.
6. Reference6. Takayama, Y. and Aoki, R. (2012). Roles of lactoferrin on skin wound healing. Biochemistry and Cell Biology, 90(3), 497-503. Reference7. Anusakthien, O. and Giannobile, W.V. (2002) Growth factor delivery to reengineer periodontal tissues. Current Pharmaceutical Biotechnology, 3, 129-139.
7. Reference8. Jin Lee, S. (2000). Cytokine delivery and tissue engineering. Yonsei Medical Journal, 41(6), 704-719.
8. Reference9. Ornitz, D. M., Itoh, N. (2001). Fibroblast growth factors. Genome Biology, 2(3), 3005-1.

9. Reference10. Casey-Sawicki, K., Zhang, M., Kim, S., Zhang, A., Zhang, S., Swarts, S., Vidyasagar, S., Zhang, L., Zhang, A., Okunieff, P. (2014). A Basic Fibroblast Growth Factor Analog for Protection and Mitigation against Acute Radiation Syndromes. Health Physics, 106(6), 704-712.
10. Reference11. Teven, C. M., Farina, E. M., Rivas, J., Reid, R. R. (2014). Fibroblast growth factor (FGF) signaling in development and skeletal diseases. Genes & Diseases, 1(2), 199–213.
11. Reference12. Yıldırım, A., Tunik, S., Çetin, Ç. ve Akkuş, M. (2009). Osteogenezde Fibroblast Büyüme Faktörleri (FGF) ve Kemik Morfogenetik Proteinlerin (Kmp) rolü. İnönü Üniversitesi Tıp Fakültesi Dergisi, 16(2), 135-140.
12. Reference13. Kobayashi, F., Matsuzaka, K. and Inoue, T. (2015). The effect of basic fibroblast growth factor on regeneration in a surgical wound model of rat submandibular glands. International Journal of Oral Science, 8(1), 16-23.
13. Reference14. Özler, M., Şimşek, K., Topal, T., Öter, Ş. ve Korkmaz, A. (2010). Pinealektomili Ratlarda Yara İyileşmesi. Gülhane Tıp Dergisi, 52, 181-184. Reference15. Luft, R. and Minkowski, O. (1989). Discovery of the pancreatic origin of diyabetes. Diyabetologia, 32(7), 399-401.
14. Reference16. Baynes, J. W., Thorpe, S. R. (1999). Role of oxidative stress in diabetic complications: a new perspective on an old paradigm. Diabetes, 48(1), 1–9.
15. Reference17. Dandona, P., Thusu, K., Cook, S., Snyder, B., Makowski, J., Armstrong, D. and Nicotera, T. (1996). Oxidative damage to DNA in diabetes mellitus. The Lancet, 347(8999), 444-445.
16. Reference18. Aslan, D., Onat, T., Emerk, K. ve Sözmen, E. Y., (2006). İnsan Biyokimyası (İkinci Baskı), Ankara: Palme Yayıncılık. 691-699, 714.
17. Reference19. Pitocco, D., Zaccardi, F., Di Stasio, E., Romitelli, F., Santini, S. A., Zuppi, C., Ghirlanda, G. (2010). Oxidative stress, nitric oxide and diabetes. The review of Diabetic Studies, 7(1), 15-25
18. Reference20. Kurtel, H., Granger, D. N., Tso, P., Grisham, M. B. (1992). Vulnerability of intestinal interstitial fluid to oxidant stress. American Journal of Physiology-Gastrointestinal and Liver Physiology, 263(4), 573-578.
19. Reference21. Miranda, K. M., Espey, M. G., Wink, D. A. (2001). A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric oxide, 5(1), 62-71.
20. Reference22. Aydemir, B., Sarı, E.K. (2009). Antioksidanlar ve Büyüme Faktörleri ile İlişkisi, Kocatepe Veteriner Dergisi, 2(2), 56-60.
21. Reference23. Altan, N., Dinçel, A. S., and Koca, C. (2006). Diabetes Mellitus ve Oksidatif Stres, Türkiye Biyokimya Dergisi., 31(2), 51-56.
22. Reference24. Çetin, M. ve Çapan, Y. (2004). Bazik Fibroblast Büyüme Faktörü ve Formülasyonlarında Yeni Yaklaşımlar, Hacettepe Üniversitesi Eczacılık Fakültesi Dergisi, 24(2), 107-124.
23. Reference25. Yazıcı, A. E., Paşaoğlu, H., Yücel, D., Çelebi, N., Bakır, F., Özkaya, M. (2002). Tip II Diabetes Mellituslu Hastalarda Plazma Total Tiyol ve Eritrosit Redükte Glutatyon Düzeyleri. Turkiye Klinikleri Journal of Medical Sciences, 22(5), 487-492.
24. Reference26. Hu, M. L. (1994). Measurement of protein thiol groups and glutathione in plasma. In Methods in enzymology. 233, 380-385
25. Reference27. Karabağ, F. (2010). Deneysel Olarak Hipertiroidizm Olusturulan Ratlarda Kafeik Asit Fenetil Ester’in Plazma Homosistein, Asimetrik Dimetil Arjinin, Nitrik Oksit ve Lipid Profili Üzerine Etkilerinin Araştırılması, Doktora Tezi, Afyon Kocatepe Üniversitesi, Veteriner Fakültesi, Afyonkarahisar, 16-17.
26. Reference28. Chin, G. A., Diegelmann, R. F., Schultz, G. S. (2005). Cellular and molecular regulation of wound healing. Basic and Clinical Dermatology, 17-37.
27. Reference29. Memişoğulları, R., (2006). Diyabette serbest radikallerin rolü ve antioksidanların etkisi, Düzce Tıp Fakültesi Dergisi, 3, 30-39.