I n this study, a novel enzyme system based on ferritin nanocages was designed. α-Amylase was covalently crosslinked on surface of ferritin nanocages by EDC/NHS. The activity of immobilized enzyme was monitored by using UV-vis spectrophotometer. The optimum temperature of immobilized enzyme shifted from 50 oC to 70 oC due to the ferritin nanocages. In addition, The Km and Vmax values of immobilized enzymes were 5.19 mg mL-1 and 3.3x10-5 U mg-1, respectively. This novel enzyme system displayed higher catalytic activity and enhanced stability
Ferritin nanocages α-amylase Enzyme immobilization EDC/NHS Covalently crosslinked
Birincil Dil | İngilizce |
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Bölüm | Research Article |
Yazarlar | |
Yayımlanma Tarihi | 29 Haziran 2018 |
Yayımlandığı Sayı | Yıl 2018 Cilt: 5 Sayı: 2 |
Hittite Journal of Science and Engineering Creative Commons Atıf-GayriTicari 4.0 Uluslararası Lisansı (CC BY NC) ile lisanslanmıştır.