The Effects of Oxygen Availability in the Seed Container during Storage on Seed Germination in Tomato, Onion, Cabbage, and Marrow Seeds

Abstract

This research was conducted to test the effect of oxygen content (low O2, high O2, air) during hermetic seed storage at 20±2°C over 8 and 12 months on seed germination and seedling root and shoot length in tomato, onion, cabbage, and marrow seeds. Samples with low oxygen storage had higher seed germination as well as longer root and shoot lengths than both control and high oxygen storage. When the storage period extended from 8 to 12 months, the germination percentages also reduced. However, these results varied among the species. The greatest advantage of low oxygen storage was obtained in tomatoes, which exhibited 15% and 9% higher germination compared to the control after 8 and 12 months of storage, respectively. The longest root and shoot lengths of 6.4 cm and 11.6 cm, respectively, were obtained from the low oxygen storage treatments. A similar positive effect of low oxygen storage was observed in onion and cabbage seeds but not in marrows. Results indicated that oxygen level in the packets during storage can be an effective component to maintain high seed germination and seedling growth potential (seed vigour). The difference in the effect on different species is a matter of further research.

Keywords

• Germination
• Longevity
• Oxygen
• Seedling
• Vegetable seeds

References

1. Barzali, M., Lohwasser, U., Niedzielski, M., & Börner, A. (2005). Effects of different temperatures and atmospheres on seed and seedling traits in a long-term storage experiment on rye (Secale cereale L.). Seed Science and Technology, 33:713–721.
2. Basak, O., Demir, I., Mavi, K., & Matthews, S. (2006). Controlled deterioration test for predicting seedling emergence and longevity of pepper (Capsicum annuum L.) seed lots. Seed Science and Technology, 34:723-734.
3. Buijs, G., Willems, L.A.J., Kodde, J., Groot, S.P.C., & Bentsink, L. (2020). Evaluating eppo method for seed longevity analyses in Arabidopsis. Plant Science, 301:110644.
4. Demir, I., Ermis, S., Mavi, K., & Matthews, S. (2008). Mean germination time of pepper seed lots (Capsicum annuum) predicts size and uniformity of seedlings in germination tests and transplant modules. Seed Science and Technology, 1:21-30.
5. De Vitis, M., Hay, F.R., Dickie, J.B., Trivedi, C., Choi, J., & Fiegener, R. (2020). Seed storage: maintaining seed viability and vigour for restoration use. Restoration Ecology, 28: S249–S255.
6. Ellis, R.H., & Roberts, E.H. (1980). Improved equations for the prediction of seed longevity. Annals of Botany, 45:13–30.
7. Ellis, R.H., & Hong, T.D. (2007). Seed longevity – moisture content relationships in hermetic and open storage. Seed Science and Technology, 35:423–431.
8. Gonzales-Benito, M.E., Perez-Garcia, F., Tejeda, G., & Gomez-Campo, C. (2011). Effect of the gaseous environment and water content on seed viability of four Brassicaceae species after 36 years storage. Seed Science and Technology, 13:443-451.

9. Groot, S.P.C., Surki, A.A., De Vos, R.C.H., & Kodde, J. (2012). Seed storage at elevated partial pressure of oxygen, a fast method for analysing seed ageing under dry conditions. Annals of Botany, 110:1149–1159.
10. Groot, S.P.C., De Groot, L., Kodde, J., & Van Treuren, R. (2015). Prolonging the longevity of ex situ conserved seeds by storage under anoxia. Plant Genetic Resources, 13:18-26.
11. Han, B., Fernandez, V., Pritchard, H.W., & Colville, L. (2021). Gaseous environment modulates volatile emission and viability loss during seed artificial ageing. Planta, 253:106.
12. Hay, F.R., Rezaei, S., & Buitink, J. (2022). Seed moisture isotherms, sorption models, and longevity. Frontiers in Plant Science, 13.
13. Ibrahim, A.E., Roberts, E.H., & Murdoch, A.J. (1983). Viability of lettuce seeds. Journal of Experimental Botany, 34:631-640.
14. ISTA. (2022). International Rules for Seed Testing, International Seed Testing Association, Welliselen, Switzerland.
15. Kranner, I., Chen, H., Pritchard, H.W., Pearce, S.R., & Birtic, S. (2011). Internucleosomal DNA fragmentation and loss of RNA integrity during seed ageing. Plant Growth Regulation, 63:63–72.
16. Pirredda, M., Gonzales-Benito, M. E., Martin, C., & Mira, S. (2020). Genetic and epigenetic stability in rye seeds under different storage conditions: ageing and oxygen effect. Plants, 9:393.
17. Prasad, C.T.M, Kodde, J., Angenent, G.C., De Vos, R.C.H., Diez-Simon, C., Mumm, R., Hay, F.R., Siricharoen, S., Yadava, D.K., & Groot, S.P.C. (2022). Experimental rice seed aging under elevated oxygen pressure: Methodology and mechanism. Frontiers in Plant Science, 13:1050411.
18. Roberts, E.H., & Abdalla, F.H. (1968). The influence of temperature, moisture and oxygen on period of seed viability in barley, broad beans, and peas. Annals of Botany, 32:97–117.
19. Sano, N., Rajjou, L., North, H.M., Debeaujon, I., Marion-Poll, A., & Seo, M. (2016). Staying alive: Molecular aspects of seed longevity. Plant and Cell Physiology, 57:660–674.
20. Schwember, A.R., & Bradford, K.J. (2011). Oxygen interacts with priming, moisture content and temperature to affect the longevity of lettuce and onion seeds. Seed Science Research, 21:175–185.
21. Tahir, A., Afzal, I., Khalid, E., Razzaq, M., & Arif, M.A.R. (2023). Rice seed longevity in the context of seed moisture contents and hypoxic conditions in the storage environment. Seed Science Research, 33:39-49.
22. Zulfikar, F. (2021). Effect of seed priming on horticultural crops. Scientia Horticulturae, 286:11097.