Comparative Evaluation of Biochemical Responses of Callus Cultures of Cichorium endivia L. and Cichorium intybus L. to In vitro Salinity Stress

Damla Turan Büyükdinç; Dilruba Tabay Sümbül

doi:10.16882/hortis.1998170

Comparative Evaluation of Biochemical Responses of Callus Cultures of Cichorium endivia L. and Cichorium intybus L. to In vitro Salinity Stress

Abstract

In this study, the responses of callus cultures of Cichorium endivia L. and Cichorium intybus L. to in vitro salt stress (0, 50, 100, and 150 mM NaCl) on MS medium were comparatively investigated. Callus morphology (structure and color) and growth parameters (fresh and dry weight) were evaluated alongside key biochemical indicators, including hydrogen peroxide (H₂O₂) and malondialdehyde (MDA) contents, antioxidant enzyme activities (Superoxide dismutase: SOD, Catalase: CAT, and Peroxidase: POD), and soluble protein levels. Principal component analysis (PCA) and heatmap visualization were applied to elucidate relationships among the measured variables and treatment effects. The results demonstrated that increasing salinity significantly influenced both morphological and biochemical responses, with clear interspecific differences. Cichorium intybus exhibited comparatively higher tolerance, as enhanced CAT and POD activities partially alleviated oxidative stress at moderate salinity levels (50-100 mM NaCl). In contrast, C. endivia showed pronounced oxidative stress responses even at lower salt concentrations, and no callus formation was observed at 150 mM NaCl. Overall, the findings indicate that C. intybus displays greater salinity tolerance under in vitro conditions, highlighting its potential for future stress physiology and breeding studies.

Keywords

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Details

Primary Language

English

Subjects

Horticultural Production (Other)

Journal Section

Research Article

Authors

Damla Turan Büyükdinç ^*
0000-0002-2776-1008
Türkiye

Dilruba Tabay Sümbül
0000-0001-8406-0155
Türkiye

Publication Date

August 14, 2026

Submission Date

April 22, 2026

Acceptance Date

July 15, 2026

Published in Issue

Year 2026 Volume: 43 Number: 2

DOI

https://doi.org/10.16882/hortis.1998170

IZ

https://izlik.org/JA74ZR42JW

Cite

RIS / Bibtex

APA

Turan Büyükdinç, D., & Tabay Sümbül, D. (2026). Comparative Evaluation of Biochemical Responses of Callus Cultures of Cichorium endivia L. and Cichorium intybus L. to In vitro Salinity Stress. Horticultural Studies, 43(2), 50-60. https://doi.org/10.16882/hortis.1998170

AMA

1.Turan Büyükdinç D, Tabay Sümbül D. Comparative Evaluation of Biochemical Responses of Callus Cultures of Cichorium endivia L. and Cichorium intybus L. to In vitro Salinity Stress. HortiS. 2026;43(2):50-60. doi:10.16882/hortis.1998170

Chicago

Turan Büyükdinç, Damla, and Dilruba Tabay Sümbül. 2026. “Comparative Evaluation of Biochemical Responses of Callus Cultures of Cichorium Endivia L. and Cichorium Intybus L. To In Vitro Salinity Stress”. Horticultural Studies 43 (2): 50-60. https://doi.org/10.16882/hortis.1998170.

EndNote

Turan Büyükdinç D, Tabay Sümbül D (August 1, 2026) Comparative Evaluation of Biochemical Responses of Callus Cultures of Cichorium endivia L. and Cichorium intybus L. to In vitro Salinity Stress. Horticultural Studies 43 2 50–60.

IEEE

[1]D. Turan Büyükdinç and D. Tabay Sümbül, “Comparative Evaluation of Biochemical Responses of Callus Cultures of Cichorium endivia L. and Cichorium intybus L. to In vitro Salinity Stress”, HortiS, vol. 43, no. 2, pp. 50–60, Aug. 2026, doi: 10.16882/hortis.1998170.

ISNAD

Turan Büyükdinç, Damla - Tabay Sümbül, Dilruba. “Comparative Evaluation of Biochemical Responses of Callus Cultures of Cichorium Endivia L. and Cichorium Intybus L. To In Vitro Salinity Stress”. Horticultural Studies 43/2 (August 1, 2026): 50-60. https://doi.org/10.16882/hortis.1998170.

JAMA

1.Turan Büyükdinç D, Tabay Sümbül D. Comparative Evaluation of Biochemical Responses of Callus Cultures of Cichorium endivia L. and Cichorium intybus L. to In vitro Salinity Stress. HortiS. 2026;43:50–60.

MLA

Turan Büyükdinç, Damla, and Dilruba Tabay Sümbül. “Comparative Evaluation of Biochemical Responses of Callus Cultures of Cichorium Endivia L. and Cichorium Intybus L. To In Vitro Salinity Stress”. Horticultural Studies, vol. 43, no. 2, Aug. 2026, pp. 50-60, doi:10.16882/hortis.1998170.

Vancouver

1.Damla Turan Büyükdinç, Dilruba Tabay Sümbül. Comparative Evaluation of Biochemical Responses of Callus Cultures of Cichorium endivia L. and Cichorium intybus L. to In vitro Salinity Stress. HortiS. 2026 Aug. 1;43(2):50-6. doi:10.16882/hortis.1998170