Genotype Effect as One of the Affecting Factors on The Success of Anther Culture in Eggplant (Solanum melongena L.)

Year 2022, Volume: 39 Issue: 2, 41 - 47, 13.06.2022

Hayati Bat Fatma Nur Altındağ Merve Arefe Yiğit Şeküre Şebnem Ellialtıoğlu Nuray Çmlekçioğlu

https://doi.org/10.16882/hortis.1119745

Cited By: 1

Abstract

Anther culture technique is one of the commonly used method in order to obtain haploid plants in eggplant. It is known that androgenic response in eggplant is variable and one of the most important factors determining success is the genotype effect. Low or insufficient embryogenic response limits the use of double haploid technology in breeding programs. Therefore, determining the embryogenic responses of genotypes is a step for the use of this technology in breeding studies. In this study, 61 eggplant genotypes at various breeding stages (F1, F2, and F3) in the gene pool of United Genetics Vegetable Seeds Company (Mustafakemalpaşa, Bursa, Turkey) were evaluated in terms of androgenic responses. Significant differences were determined among eggplant genotypes in terms about embryo induction and conversion rates of embryos to full plantlets. In the study, responsiveness to anther culture based on genotype ranged from 0.0%-21.58%. The conversion rate of induced embryos to plants varied between 0.0% and 100%.

Keywords

Androgenesis, Double haploid, Embryo, Genotype

References

• Başay, S., & Ellialtıoglu, Ş.Ş. (2013). Effect of genotypical factors on the effectiveness of anther culture in eggplant (Solanum melongena L.). Turkish Journal of Biology. 37:499-505.
• Benelli, C., Germana, M.A., Ganino, T., Beghe, D., & Fabbri, A. (2010). Morphological and anatomical observations of abnormal somatic embryos from anther cultures of Citrus reticulate. Biologia Plantarum, 54:224-230.
• Bhattacharya, A., Sonone, Y., & Char, B. (2019). Improvement in tissue culture-assisted induction of double haploidy in brinjal (Solanum melongena L.) Journal of Applied Horticulture, 21:178-181.
• Denli, N. (2019). Expansion of genetic basis via interspecies crossing of pepper (Capsicum annuum) and determination the inheritance of androgenesis. PhD Thesis, Çukurova University, Adana, Türkiye.
• Dumas de Vaulx, R., & Chambonnet, D. (1982). Culture in vitro antheres aubergine (Solanum melongena L.): stimulation de la production de plantes au moyen de traitements a + 35 degres C associes a de faibles teneurs en substances de croissance Agronomie, EDP Sciences, 2:983-988.
• Dunwell, J.M. (1976). A comparative study of environmental and developmental factors which influence embryo induction and growth in cultured anthers of Nicotiana tabacum. Environmental and Experimental Botany, 16:109-118.
• Ellialtioglu, Ş., Sarı, N., & Abak, K. (2001). In: Babaoğlu M, Gurel E, Ozcan, S. editors. Plant Biotechnology-I Tissue Culture and its Applications. Haploid Plant Production, pp137-189 (in Turkish).
• Foroughi-Wehr, B., Friedt, W., & Wenzel, G. (1982). On the genetic improvement of androgenetic haploid formation in Hordeum vulgare L. Theoretical and Applied Genetic, 62:233-239.
• Hagberg, A., & Hagberg, G. (1980). High frequency of spontaneous haploids in the progeny of an induced mutation in barley. Hereditas, 93:341-343.
• Hale, B., Ferrie, A.M.R., Chellamma, S., Samuel, J.P., & Phillips, G.C. (2022). Androgenesis-based doubled haploidy: Past, present, and future perspectives. Plant Science, 12:751230.
• Karakullukçu, Ş. (1991). Investigation of some factors inducing in vitro androgenesis and haploid plant development in different eggplant genotypes. PhD Thesis, Ankara University, Türkiye.
• Kermicle, J.L. (1969). Androgenesis conditioned by a mutation in maize. Science,166:1422-1424.
• Khatun, F., Meah, M.B., & Nasiruddin, K.M. (2006). Regeneration of eggplant through anther culture. Pakistan Journal of Biological Sciences, 9:48-53.
• Kim, M.Z., & Jang I.C. (2000). Rapid assesment of microspore development stage in pepper using DAPI and ferric cloride. Journal of Plant Biotechnology, 2:129-134.
• Mir, R., Calabuig-Serna, A., & Segui-Simarro, J.M. (2021). Doubled haploids in eggplant. Biology, 10:685.
• Munoz-Amatriain, M., Svensson, J.T., Castillo, A.M., Close, T.J., & Valles, M.P. (2009). Microspore embryogenesis: assignment of genes to embryo formation and green vs. albino plant production. Functional and Integrative Genomics, 9:311–323.
• Rivas-Sendra, A., Campos-Vega, M., Calabuig-Serna, A., & Segui-Simarro, J.M. (2017). Development and characterization of an eggplant (Solanum melongena) doubled haploid population and a doubled haploid line with high androgenic response. Euphytica, 213:89.
• Rivas-Sendra, A., Corral-Martinez, P., Porcel, R., Camacho-Fernandez C., Calabuig-Serna, A. & Segui-Simarro, J.M. (2019). Embryogenic competence of microspores is associated with their ability to form a callosic, osmoprotective subintinal layer. Journal of Experimental Botany, 70:1267–1281.
• Rivas-Sendra, A., Corral Martínez, P., Camacho-Fernández, C., Porcel, R., & Seguí-Simarro, J.M. (2020). Effects of growth conditions of donor plants and in vitro culture environment in the viability and the embryogenic response of microspores of different eggplant genotypes. Euphytica, 216:1-15.
• Seeja, G., & Sreekumar, S. (2020). Doubled haploids in genetic improvement: A review. International Journal of Recent Scientific Research, 11:36941-36949.
• Nielsen, F. (2016). Hierarchical clustering. In: Introduction to HPC with MPI for data Science.Vol.8. ISBN: 978-3-319-21902-8. Springer. p. 282.
• Vural, G.E., Ari, E., Zengin, S., & Ellialtioglu, S. (2019). Development of androgenesis studies on eggplant (Solanum melongena L.) in Turkey from past to present. In M. Hasanuzzaman, M. C. M. T. Filho, M. Fujita, & T. A. R. Nogueira (Eds.), Sustainable crop production. IntechOpen.
• Vural, G.E., & Ari, E. (2020). Triple synergistic effect of maltose, silver nitrate and activated charcoal on high embryo yield of eggplant (Solanum melongena L.) anther cultures. Scientia Horticulturae, 272:109472.