The Effect of the Application of Mycorrhiza on Vegetative Growth, Mineral Element Intake, and Some Biochemical Characteristics of Strawberry Seedlings under Lime Stress

Year 2023, Volume: 40 Issue: 2, 62 - 71, 01.08.2023

Gülden Balcı

https://doi.org/10.16882/hortis.1330523

Cited By: 1

Abstract

This study aims to determine the effects of vesicular–arbuscular mycorrhiza (VAM) applications on vegetative growth, mineral element intake, and some biochemical characteristics of strawberry seedlings grown under lime stress conditions. The experiment was conducted with frigo seeds of "Albion" strawberry cultivar in pots filled with 1% lime mixture and 1:1 ratio of peat and perlite. In the uprootings performed in three different stages (four leaved, blooming, and fruit stages) to examine the biochemical effects of mycorrhiza applications against the lime stress, vegetative growth criteria (leaf chlorophyll and anthocyanin content, area, crown diameter, fresh and dry plant weights) and mineral contents in the plant parts (leaf, crown, and root) were determined. The proline, total phenolic content, and malondialdehyde (MDA), end product of the lipid peroxidation, analyses were conducted on the leaf samples taken in these uprooting. In all three stages, an increase in crown diameter and leaf area was determined. In uprooting periods, proline and total phenolic amounts increased, and, on the other hand, MDA decreased. Microelement intake, which decreased with the lime application, was detected to be increased with mycorrhiza applications. At the end of the experiment, mycorrhiza application was observed to lessen the effect of lime stress on strawberry seedlings.

Keywords

Calcium stress, Lipid peroxidation, Mycorrhiza, Strawberry, Proline

References

• Abbaspoura, H., Saeidi-Sarb, S., Afsharia, H., & Abdel-Wahhabc, M.A. (2012). Tolerance of mycorrhiza infected Pistachio (Pistacia vera L.) seedling to drought stress under glasshouse conditions. Journal of Plant Physiology, 169:704-709.
• Akay, A., & Kararslan E. (2012). The effect of different doses phosphorus and iron fertilizer application on leaf chlorophyll content in mycorrhiza inoculated bitter melon (Momordica charantia) plant. Iğdır University Journal of Institute Science & Technology, 2(3):103-108 (in Turkish).
• Aktaş, L.Y., & Akça, H. (2015). Effects of proline treatment on inducing drought tolerance of laurel seedlings. Cumhuriyet University Faculty of Science Science Journal, 36(1):17-27 (in Turkish).
• Almaca, A. (2014). The importance of mycorrhizae in agricultural production. Harran Agriculture and Food Scince Journal, 18(2):56-65 (in Turkish).
• Avioa, L., Sbranaa, C., Giovannettib, M., & Frassinettia, S. (2017). Arbuscular mycorrhizal fungi affect total phenolics content and antioxidant activity in leaves of oak leaf lettuce varieties. Scientia Horticulturae, 224:625-671.
• Bahmanbiglo, F.A., & Eshgh, S. (2021). The effect of hydrogen sulfide on growth, yield and biochemical responses of strawberry (Fragaria × ananassa cv. Paros) leaves under alkalinity stress. Scientia Horticulturae, 282:110013.
• Balcı, G., Koç, A., Keles, H., & Kılıç, T. (2017). Evaluation of some strawberry day neutral cultivars performance in Yozgat. Fruit Science, 4(2):6-12 (in Turkish).
• Baozhong, Y., Wang, Y., Liu, P., Hu, J., & Zhen, W. (2010). Effects of vesicular arbuscular mycorrhiza on the protective system in strawberry leaves under drought stress. Frontiers of Agriculture in China, 4:165–169.
• Bates, W.R.P., & Teare, I.D. (1973). Rapid determination of free proline for water-stress studies. Plant and Soil, 39:205-207.
• Bavaresco, L., & Fogher, C. (1995). Lime-induced chlorosis of grapevine as affected by rootstock and root infection with arbuscular mycorrhiza and Pseudomonasf fluorescens. Vitis, 35(3):119-123.
• Bayözen, A., & Yıldız, A. (2008). Determination of mycorrhizae interactions and pathogenicity of rhizoctonia solani kühn isolated from strawberry and Xanthium strumarium. Turkish Journal of Biology, 32:53-57.
• Borkowska, B. (2002). Growth and photosynthetic activity of micropropagated strawberry plants inoculated with endomycorrhizal fungi (AMF) and growing under drought stress. Acta Physiologiae Plantarum, 24(4):365-370.
• Borowicz, V.A. (2010). The impact of arbuscular mycorrhizal fungi on strawberry tolerance to root damage and drought stress. Pedobiologia, 53:265-270.
• Büyük, İ., Aydin, S.S., & Aras, S. (2012). Molecular responses of plants to stress conditions. Turkish Bulletin of Hygiene and Experimental Biology, 69(2):97-110 (in Turkish).
• Campanelli, A., Ruta, C., De Mastro, G., & Morone-Fortunato, I., (2013). The role of arbuscular mycorrhizal fungi in alleviating salt stress in Medicago sativa L. var. icon. Symbiosis, 59:65–76.
• Cekic, C., & Yilmaz, E. (2011). Effect of arbuscular mycorrhiza and different doses of phosphor on vegetative and generative components of strawberries applied with different phosphor doses in soilless culture. African Journal of Agricultural Research, 6(20):4736-4739.
• Cetin, E.S., & Daler, S. (2017). Mechanism of resistance against alkaline stress by plant growth-promoting Rhizobacteria in Vitis. International Journal of Multidisciplinary Research and Development, 4(7):462-466.
• Chen, T.H., & Murata, N. (2002). Enhancement of tolerance of abiotic stress by metabolic engineering of betaines and other compatible solutes. Current Opinion in Plant Biology, 5(3):250-257.
• Cohen, J. (1988). The t test for means. Statistical Ppower Analysis for the Behavioural Sciences, 5:250-257.
• Daugaard, H. (2001). Nutritional status of strawberry cultivars in organic production. Journal of Plant Nutrition, 24(9):1337–1345.
• Demirsoy, L., Demirsoy, H., Ersoy, B., Balci, G., & Kizilkaya, R., (2010). Seasonal variation of NPK and Ca content of leaf, crown and root of Sweet Charlie strawberry under different irradiation. Zemdirbyste-Agriculture, 97(1):23-32.
• Demirsoy, L., Demirsoy, H., & Balci, G. (2012). Different growing conditions affect nutrient content, fruit yield and growth in strawberry. Pakistan Journal of Botany, 44(1):125-129.
• Ersoy, B., Demirsoy, H. (2006). Study on effects of different shading treatments on seasonal variation of some nutrients in ‘Camarosa’ strawberry. Journal of Agricultural Faculty of Ondokuz Mayıs University, 21(1):82-88 (in Turkish).
• Ertan, E., Kılınç, S., Yıldız, A., & Şirin, U. (2007). Effects of mycorrhiza application on plant growth and yield in strawberry growing in soilless environment. Türkiye V. Ulusal Bahçe Bitkileri Kongresi (04-07 Eylül 2007). Erzurum, p:723 (in Turkish).
• Erzurumlu, G.S., & Kara, E.E. (2014). Studies on mycorrhiza in Turkey. Turkish Journal of Scientific Reviews, 7(2):55-65 (in Turkish).
• Falandysz, J., Szymczyk, K., Ichihashi, H., Bielawski, L., Gucia, M., Frankowska, A., & Yamasak. S.I. (2001). ICP/MS and ICP/AES elemental analysis of edible wild mushrooms growing in Poland. Food Additives and Contaminants, 18(6):503-513.
• Farrant, J.M. (2000). A comparison of mechanisms of desiccation tolerance among three angiosperm resurrection plant species. Plant Ecology, 151:29-39.
• Gianinazzi, S., & Schüepp, H. (1994). Impact of Arbuscular Mycorrhizas on Substainable Agriculture and Natural Ecosystems. Springer Basel AG, ISBN 978-3-0348-9654-2.
• Giri, B., Kapoor, R., & Mukerji, K.G. (2003). Influence of arbuscular mycorrhizal fungi and salinity on growth, biomass, and mineral nutrition of Acacia auriculiformis. Biology and Fertility of Soils, 38:170–175.
• Gupta, R., & Krishnamurthy, K.V. (1996). Response of mycorrhizal and nonmycorrhizal Arachis hypogaea to NaCl and acid stress. Mycorrhiza, 6:145–149.
• Hazelton, P., & Murphy, B. (2007). Interpreting Soil Test Results. What Do All the Numbers Mean? Published by CSIRO Publisjing.160 pp.
• Hazzoumi, Z., Moustakime, Y., Elharchli, H., & Joutei, A.K. (2015). Effect of arbuscular mycorrhizal fungi (AMF) and water stress on growth, phenolic compounds, glandular hairs, and yield of essential oil in basil (Ocimum gratissimum L). Chemical and Biological Technologies in Agriculture, 2(10):1-11.
• Hodges, D.M., DeLong, J.M., Forney, C.F., & Prange, R.K. (1999). Improving the thiobarbituric acid-reactivesubstances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta, 207:604–11.
• Johnston, J.W., Harding, K., & Benson, E.E. (2007). Antioxidant status and genotypic tolerance of Ribes in vitro cultures to cryopreservation. Plant Science, 172:524- 534.
• Kacar, B., 2012. Soil Analysis. Nobel Publisher, ISBN 6053951841, Ankara, Türkiye, p:466 (in Turkish).
• Koç, A. (2015). Effect of plant growth-promoting bacteria and arbuscular mycorrhizal fungi on lipid peroxidation and total phenolics of strawberry (Fragaria × ananassa ‘San Andreas’) under salt stress. Turkish Journal of Agriculture and Forestry, 39:992-998.
• Koç, A., Balcı, G., Ertürk, Y., Keles, H., Bakoğlu, N., & Ercişli, S. (2016). Influence of arbuscular mycorrhizae and plant growth promoting rhizobacteria on proline content, membrane permeability and growth of strawberry (Fragaria × ananassa Duch.) under salt stress. Journal of Applied Botany and Food Quality, 89:89-97.
• Krishna, H., Singh, S.K., Minakshi, Patel, V.B., Khawale, R.N., Deshmukh, P.S., & Jindal, P.C. (2006). Arbuscular-mycorrhizal fungi alleviate transplantation shock in micropropagated grapevine (Vitis vinifera L.), The Journal of Horticultural Science and Biotechnology, 81(2):259-263.
• Krupa, Z., & Baszynski, T. (1989). Acyl lipid composition of thylakoid membranes of cadmium–treated tomato plants. Acta Physiol Plantarum, 11:111-6.
• Latef, A. A. H. A., Hashem, A., Rasool, S., Abd_Allah, E.F., Alqarawi, A.A., Egamberdieva, D., Jan, S., Anjum, N.A., & Ahmad, P. (2016). Arbuscular mycorrhizal symbiosis and abiotic stress in plants: A review. Journal of Plant Biology, 59:407-426.
• Matsubara, Y., Ishigaki, T., & Koshikawa, K. (2009). Changes in free amino acid concentrations in mycorrhizal strawberry plants. Scientia Horticulturae, 119:392–396.
• May, G.M., & Pritts, M.P. (1990). Strawberry nutrition. Advances in Strawberry Production, 9:10-24.
• May, G.M., Pritts, M.P., & Kelly, M.J. (1994). Seasonal patterns of growth and tissue nutrient content in strawberries. Journal of Plant Nutrition, 17(7):1149-1162.
• Medeiros, C.A.B., Clark, R.B., & Ellis, J.R. (1994). Effects of excess aluminum on mineral uptake in mycorrhizal sorghum. Journal of Plant Nutrition, 17(8):1399-1416.
• Moradtalab, N., Hajiboland, R., Aliasgharzad, N., Hartmann, T.E., & Neumann, G. (2019). Silicon and the association with an arbuscular-mycorrhizal fungus (Rhizophagus clarus) mitigate the adverse effects of drought stress on strawberry. Agronomy, 9(41):2-20.
• Nouairi, I., Ben Ammar, W., Ben Youssef, N., Ben Miled Daoud, D., & Habib Ghorbal, M. (2006). Comparative study of cadmium effects on membrane lipid composition of Brassica juncea and Brassica napus leaves. Plant Science, 170:511-9.
• Pešaković, M., Milenković, S., Đukić, D., Mandić, L., Karaklajić-Stajić, Ž., Tomić, J., & Miletić, N. (2016). Phenolic composition and antioxidant capacity of integrated and conventionally grown strawberry (Fragaria × ananassa Duch.). Horticultural Science (Prague), 43(1):17-24.
• Quariti, O., Boussama, N., Zarrouk, M., Cherif, A., & Ghorbal, M.H. (1997). Cadmium and copperinduced changes in tomato membrane lipids. Phytochemistry, 45:1343-50.
• Rontein, F.D., Basset, G., & Hanson, A.D. (2002). Metabolic engineering of osmoprotectant accumulation in plants. Metabolic Engineering, 4:49-56.
• Sharma, M.P., & Adholeya, A. (2004). Effect of arbuscular mycorrhizal fungi and phosphorus fertilization on the post vitro growth and yield of micropropagated strawberry grown in a sandy loam soil. Canadian Journal of Botany, 82:322-328.
• Sinclair, G., Charest, C., Dalpe, Y., & Khanizadeh, S. (2014). Influence of colonization by arbuscular mycorrrhizal fungi on three strawberry cultivars under salty conditions. Agricultural and Food Science, 23:146-158.
• Singleton, V.L., & Rossi, J.R. (1965). Colorimetry of total phenolics with phosphomolybdic phosphotungstic acid. American Journal of Enology and Viticulture, 16:144-158.
• Sönmez, F., Çığ, F., Erman, M., & Tüfenkçi, Ş. (2013). Effects of zinc, salt and mycorrhiza applications on the development and the phosphorus and zinc uptake of maize. Yuzuncu Yil University Journal of Agricultural Sciences, 23(1):1–9 (in Turkish).
• Stanisavljevic, M., Gavrilovic-Damjanovic, J., Mitrovic, O., & Mitrovic, V. (1997). Dynamics and contents of minerals in some strawberry organs and tissues. Acta Horticulturae, 439(2):705-708.
• Stewart, L.I., Hamel, C., Hogue, R., & Moutoglis, P. (2005). Response of strawberry to inoculation with arbuscular mycorrhizal fungi under very high soil phosphorus conditions. Mycorrhiza, 15:612–619.
• Tüfenkci, S., Sönmez, F., & Şensoy, G.R.I. (2006). Effects of arbuscular mycorrhiza fungus inoculation and phosphorous and nitrogen fertilizations on some plant growth parameters and nutrient content of soybean. Pakistan Journal of Biological Sciences, 9(6):1121-1127.
• Vardharajula, S., Zulfikar Ali, S., Grover, M., Reddy, G., & Bandi, V. (2011). Drought-tolerant plant growth promoting Bacillus spp. effect on growth, osmolytes, and antioxidant status of maize under drought stres. Journal of Plant Interaction, 6(1):1-14.
• Yakupoğlu, T., Özturk, E., Özdemir, N., & Özkaptan, S. (2010). Effect of conditioner applications on micro nutrient content of corn plant in acidic soils. Anadolu Journal of Agriculture Science, 25(2):100-105 (in Turkish).
• Yano, K., & Takaki M. (2005). Mycorrhizal alleviation of acid soil stress in the sweet potato (Ipomoea batatas), Soil Biology & Biochemistry, 37: 1569–1572.
• Yekbun, A., & Kabay, T. (2017). The effect of drought stress on some physiologic parameters in some native and commercial tomato genotypes. Journal of the Institute of Natural & Applied Sciences, 22(2):86-96.
• Yılmaz, H., Oğuz, H.İ., & Yıldız, K. (2006). Problems of strawberry cultivation in colder areas andsome suggestions for solution. II. Üzümsü Meyveler Sempozyumu, 14-16 Eylül, Tokat, p:61-69 (in Turkish). Yılmaz, H., 2009. Strawberry. Hasat Yayıncılık, 348 p (in Turkish).
• Yong, Z., Hao-Ru, T., & Ya, L. (2008). Variation in antioxidant enzyme activities of two strawberry cultivars with short-term low temperature stress. World Journal of Agricultural Sciences, 4 (4): 58-462.
• Zhang, X., & Xiong, T. (2008). Improving Glycyrrhiza uralensis, salt tolerance with N+ ion irradiation. Russ. J. Plant Physiol. 55:344-349.