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Yıl 2023, Cilt: 40 Sayı: 3, 72 - 78, 27.11.2023
https://doi.org/10.16882/hortis.1343859

Öz

Kaynakça

  • Aleza, P., Juarez, J., Ollitrault, P., & Navarro, L. (2009). Production of tetraploid plants of non-apomictic citrus genotypes. Plant Cell Reports, 28:1837-1846.
  • Aleza, P., Juárez, J., Cuenca, J., Ollitrault, P., & Navarro, L. (2010). Recovery of Citrus triploid hybrids by embryo rescue and flow cytometry from 2x × 2x sexual hybridisation and its application to extensive breeding programs. Plant Cell Reports, 29:1023-1034.
  • Aleza, P., Froelicher, Y., Schwarz, S., Agusti, M., Hernández, M., Juárez, J., Luro, F., Navarro, L., & Ollitrault, P. (2011). Tetraploidization events by chromosome doubling of nucellar cells are frequent in apomictic citrus and are dependent on genotype and environment. Annals of Botany, 108:37-50.
  • Aleza, P., Juárez, J., Cuenca, J., Ollitrault, P., & Navarro, L. (2012). Extensive Citrus triploid hybrid production by 2x × 4x sexual hybridizations and parent-effect on the length of the juvenile phase. Plant Cell Reports, 31:1723-1735.
  • Ali, S., Khan, A.S., Raza, S.A., Naveed, R., & Rehman, U. (2013). Innovative breeding methods to develop seedless citrus cultivars. International Journal of Biosciences, 3(8):191-201.
  • Al-Naggar, A.M.M., Abdelzaher, M.H., & Shaban, A.E.A. (2009). Fruit, seed and seedling characteristics of eight newly developed interspecific hybrids of Citrus. Research Journal of Agriculture and Biological Sciences, 5(5), 639-648.
  • Batchelor, L.D. (1943). The Citrus Industry, History, Botany, and Breeding. v.2. Production of the crop (eds: Webber, H.J. and Batchelor, L.D.),University of California Press.
  • Broadbent, P., & Gollnow, B.I. (1993). Selecting disease resistant citrus rootstocks. Australian Journal of Experimental Agriculture, 33:775-780.
  • Cameron, J.W., & Frost, H.B. (1968). Genetics, breeding and nucellar embryony. In: W. Reuther, L. D. Batchelor, H.J. Webber (Eds.): The Citrus Industry. Vol. II. Anatomy, Physiology, Genetics, and Reproduction. University of California, Division of Agricultural Sciences, 325-370.
  • Carimi, F., Pasquale, F., & Puglia, A.M. (1998). In vitro rescue of zygotic embryos of Sour Orange (Citrus aurantium L.) and their detection based on RFLP analysis. Plant Breeding, 117:261-266.
  • Castle, W.S. (2010). A career perspective on Citrus rootstocks, their development andcommercialization. HortScience, 45:11-15.
  • Chagas, E.A., Pasqual, M., Ramos, J.D., Pio, L.A.S., Dutra, L.F. & Cazetta, J.O. (2005). Activated charcoal and gibberellic acid concentrations on immature embryos culture. Ciencia e Agrotecnologia, 29(6):1125-1131.
  • Deng, X.X., Yi, H.L., Li, F., & Guo, W.W. (1996). Triploid plants regenerated from crossing diploid pummelo and tangerine with allotetraploid somatic hybrid of Citrus. Proceedings of the International Society of Citriculture, 1:189-192.
  • Dolezel, J., Greilhuber, J., & Suda, J. (2007). Estimation of nuclear DNA content in plants using flow cytometry. Nature Protocols, 2, 2233-2244.
  • Ferrante, S. P., Lucretti, S., Reale, S., De Patrizio, A., Abbate, L., Tusa, N., & Scarano, M.T. (2010). Assessment of the origin of new Citrus tetraploid hybrids by means of SSR markers and PCR based dosage effects. Euphytica, 173:223–233.
  • IPGRI (1999). International Plant Genetic Resources Institute. Descriptors for Citrus. Rome, 75 p.
  • Jaskani, M.J., Khan, I.A., & Khan, M.M. (2005). Fruit set, seed development and embryo germination in interploid crosses of Citrus. Scientia Horticulturae, 107:51-57.
  • Kim, M., Kim, S.H., Kim, H.B., Park, Y.C., & Song, K.J. (2020). Some factors affecting the efficiency of hybrid embryo rescue in the ‘Shiranuhi’ mandarin. Horticultural Science and Technology, 38:271-281.
  • Kurt, Ş. (2010). Determinatıon of suitable GA3 concentration and embryo growing stages from the obtained with hybridization of immature embryo rescue in Citrus seeds. MSc. Thesis, Akdeniz University.
  • Kurt, Ş., & Ulger, S. (2014). Production of Common Sour Orange × Carrizo Citrange hybrids using embryo rescue. International Journal of Fruit Science, 14:42-48.
  • Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15:473-497.
  • Murashige, T., & Tucker, P.H. (1969). Growth factor requirements of Citrus tissue culture. Proceedings 1st International Citrus Symposium, 3:1155-1161.
  • Ozsan, M., & Cameron, J.W. (1963). Artificial culture of small Citrus embryos, and evidence against nucellar embryony in highly zygotic varieties. Proceedings of the American Society for Horticultural Science, 82:210–216.
  • Pena, L., Cervera, M., Ghorbel, R., Dominguez, A., Fagoaga, C., Juarez, J., Pina, J.A., & Navarro, L. (2007). Genetic transformation. In: I. A. Khan (Ed.): Citrus, Genetics, Breeding and Biotechnology, CABI Publications, 329-344.
  • Perez-Tornero, O., & Porras, I. (2008). Assessment of polyembryony in lemon: Rescue and in vitro culture of immature embryos. Plant Cell Tissue Organ Culture, 93:173-180.
  • Perez-Tornero, O., Cordoba, D., Moreno, M., Yuste, I., & Porras, I. (2011). Use of classical methods and tools biotecnologiccas in the genetic improvement of the lemon tree: Preliminary results. Hoticultura Global, 296, 14-17.
  • Pestana, M., Varennes, A., Abadia, J., & Faria, E.A. (2005). Differential tolerance to iron deficiency of rootstocks grown in nutrient solutional. Scientia, 104(1):25-36.
  • Raghavan, V. (1980). Embryo culture. In: Perspectives in plant cell and tissue culture. International Review of Cytology, Supplement 11B, 209-240.
  • Rodríguez, M.A., Monter, A.V., Castañeda, G.C., & Velázquez, A.G. (2004). Polyembryony and identification of Volkamerian lemon zygotic and nucellar seedlings using RAPD. Pesquisa Agropecuária Brasileira, 39(6):551-559.
  • Singh, B., Sharma, S., Rani, G., Virk, G.S., Zaidi, A.A., & Nagpal, A. (2006). In vitro flowering in embryogenic cultures of Kinnow mandarin (Citrus nobilis Lour x C. deliciosa Tenora). African Journal of Biotechnology, 5(16):1470-1474.
  • Soni, A., Dubey, A.K., Gupta, A., Sharma, R. M., Awasthi, O.P., Bharadwaj, C., & Sharma, N. (2019). Optimizing embryo age and media for enhancing hybrid seedling recovery in Sour orange (Citrus aurantium) × Sacaton citrumelo (Citrus paradisi × Poncirus trifoliata) crosses through embryo rescue. Plant Breeding, 9 pp.
  • Soost, R.K,. & Cameron, J.W. (1975). Citrus. In: Advances in Fruit Breeding. Purdue University Press, West Lafayette, 507-540.
  • Soost, R.K., & Roose, M.L. (1996). Citrus. In: J. Janick, J. M., Moore (Eds.): Fruit Breeding, Volume 1, Tree and Tropical Fruits, John Wiley, New York, 256-323. ISBN:978-0-471-31014-3.
  • Şeker, M., Tuzcu, O., & Ollitrault, P. (2003). Comparison of nuclear DNA content of Citrus rootstock populations by flow cytometry analysis. Plant Breeding, 122:169-172.
  • Tan, M., Song, J., & Deng, X. (2007). Production of two mandarin x trifoliate orange hybrid populations via embryo rescue with verification by SSR analysis. Euphytica, 157:155-160.
  • Tusa, N., Abbate, L., Ferrante, S., Lucretti, S., & Scarano, M.T. (2002). Identification of zygotic and nucellar seedlings in Citrus interploid crosses by means of isozymes, flow cytometry and ISSR-PCR. Cellular & Molecular Biology Letters, 7(2B):703-708.
  • Tuzcu, Ö. (1978). Turunçgillerde anaç ve sorunları. Çağdaş Tarım Tekniği (3), 31-35 (in Turkish).
  • Viloria, Z., Grosser, J.W., & Bracho, B. (2005). Immature embryo rescue, culture and seedling development of acid Citrus fruit derived from interploid hybridization. Plant Cell, Tissue and Organ Culture, 82, 159-167.
  • Wang, J.F., Chen, Z.G., & Lin, T.X. (1999). Observation on the embryonic development in Citrus after cross pollination. Chinese Developmental Reproductive Biology Society, 8(2):57-63.
  • Xie, K.D., Yuan, D.Y., Wang, W., Xia, Q.M., Wu, X.M., Chen, C.W., Chen, C.L., Grosser, J.W. & Guo, W.W. (2019). Citrus triploid recovery based on 2x × 4x crosses via an optimized embryo rescue approach. Scientia Horticulturae, 252: 104-109.
  • Yun, J.U., Yang, H.B., Jung, Y.H., Yun, S.H., Kim, K.S., Kim, C.S., & Song, K.J. (2007). Identification of zygotic and nucellar mandarin seedling using randomly amplified polymorphic DNA. Horticulture, Environment, and Biotechnology, 48(3):171-175.

Improvement of Citrus Rootstock Hybrids Derived by 2x × 2x Intra Crosses with the Aid of Embryo Rescue and Ploidy Detection

Yıl 2023, Cilt: 40 Sayı: 3, 72 - 78, 27.11.2023
https://doi.org/10.16882/hortis.1343859

Öz

Diploid Citrus × Poncirus hybrids have significantly contributed to citrus rootstock evaluation. In Türkiye, common sour orange rootstock is used in many different climates and soil conditions at almost 85% of citrus plantations, but this rootstock is susceptible to Citrus tristeza virus disease. This study was conducted to improve new rootstock genotypes by traditional hybridization. Common sour orange (CSO) and Common mandarin (Citrus deliciosa Ten.) (CM) were crossed with Troyer citrange (TC) while King mandarin (KM) was crossed with Carrizo citrange (CC). Embryos obtained from crosses were taken on 110, 120 and 130 days after artificial pollination (DAP), and were germinated on MT culture media. The embryos of 120 DAP of combinations had the highest germination rate within CSO×TC, 95.15%; CM×TC, 96.25%; KM×CC, 95.23%. The trifoliate rates for each combination at subculture (CSO×TC, 17.40%; CM×TC, 11.11%; KM×CC; 6.17%) were obtained from 110 DAP embryos. Survival rates of the genotypes were ranged between 72.13% and 90.28% in subculture and varied from 40.17% and 64.71% in the greenhouse. As a result of the ploidy analysis by flow cytometry, the nuclear DNA content of diploid genotypes were found between 0.78 pg/2C and 0.93 pg/2C. One of the genotypes derived from CM×TC hybridization on 120 DAP was determinated as a triploid plant.

Kaynakça

  • Aleza, P., Juarez, J., Ollitrault, P., & Navarro, L. (2009). Production of tetraploid plants of non-apomictic citrus genotypes. Plant Cell Reports, 28:1837-1846.
  • Aleza, P., Juárez, J., Cuenca, J., Ollitrault, P., & Navarro, L. (2010). Recovery of Citrus triploid hybrids by embryo rescue and flow cytometry from 2x × 2x sexual hybridisation and its application to extensive breeding programs. Plant Cell Reports, 29:1023-1034.
  • Aleza, P., Froelicher, Y., Schwarz, S., Agusti, M., Hernández, M., Juárez, J., Luro, F., Navarro, L., & Ollitrault, P. (2011). Tetraploidization events by chromosome doubling of nucellar cells are frequent in apomictic citrus and are dependent on genotype and environment. Annals of Botany, 108:37-50.
  • Aleza, P., Juárez, J., Cuenca, J., Ollitrault, P., & Navarro, L. (2012). Extensive Citrus triploid hybrid production by 2x × 4x sexual hybridizations and parent-effect on the length of the juvenile phase. Plant Cell Reports, 31:1723-1735.
  • Ali, S., Khan, A.S., Raza, S.A., Naveed, R., & Rehman, U. (2013). Innovative breeding methods to develop seedless citrus cultivars. International Journal of Biosciences, 3(8):191-201.
  • Al-Naggar, A.M.M., Abdelzaher, M.H., & Shaban, A.E.A. (2009). Fruit, seed and seedling characteristics of eight newly developed interspecific hybrids of Citrus. Research Journal of Agriculture and Biological Sciences, 5(5), 639-648.
  • Batchelor, L.D. (1943). The Citrus Industry, History, Botany, and Breeding. v.2. Production of the crop (eds: Webber, H.J. and Batchelor, L.D.),University of California Press.
  • Broadbent, P., & Gollnow, B.I. (1993). Selecting disease resistant citrus rootstocks. Australian Journal of Experimental Agriculture, 33:775-780.
  • Cameron, J.W., & Frost, H.B. (1968). Genetics, breeding and nucellar embryony. In: W. Reuther, L. D. Batchelor, H.J. Webber (Eds.): The Citrus Industry. Vol. II. Anatomy, Physiology, Genetics, and Reproduction. University of California, Division of Agricultural Sciences, 325-370.
  • Carimi, F., Pasquale, F., & Puglia, A.M. (1998). In vitro rescue of zygotic embryos of Sour Orange (Citrus aurantium L.) and their detection based on RFLP analysis. Plant Breeding, 117:261-266.
  • Castle, W.S. (2010). A career perspective on Citrus rootstocks, their development andcommercialization. HortScience, 45:11-15.
  • Chagas, E.A., Pasqual, M., Ramos, J.D., Pio, L.A.S., Dutra, L.F. & Cazetta, J.O. (2005). Activated charcoal and gibberellic acid concentrations on immature embryos culture. Ciencia e Agrotecnologia, 29(6):1125-1131.
  • Deng, X.X., Yi, H.L., Li, F., & Guo, W.W. (1996). Triploid plants regenerated from crossing diploid pummelo and tangerine with allotetraploid somatic hybrid of Citrus. Proceedings of the International Society of Citriculture, 1:189-192.
  • Dolezel, J., Greilhuber, J., & Suda, J. (2007). Estimation of nuclear DNA content in plants using flow cytometry. Nature Protocols, 2, 2233-2244.
  • Ferrante, S. P., Lucretti, S., Reale, S., De Patrizio, A., Abbate, L., Tusa, N., & Scarano, M.T. (2010). Assessment of the origin of new Citrus tetraploid hybrids by means of SSR markers and PCR based dosage effects. Euphytica, 173:223–233.
  • IPGRI (1999). International Plant Genetic Resources Institute. Descriptors for Citrus. Rome, 75 p.
  • Jaskani, M.J., Khan, I.A., & Khan, M.M. (2005). Fruit set, seed development and embryo germination in interploid crosses of Citrus. Scientia Horticulturae, 107:51-57.
  • Kim, M., Kim, S.H., Kim, H.B., Park, Y.C., & Song, K.J. (2020). Some factors affecting the efficiency of hybrid embryo rescue in the ‘Shiranuhi’ mandarin. Horticultural Science and Technology, 38:271-281.
  • Kurt, Ş. (2010). Determinatıon of suitable GA3 concentration and embryo growing stages from the obtained with hybridization of immature embryo rescue in Citrus seeds. MSc. Thesis, Akdeniz University.
  • Kurt, Ş., & Ulger, S. (2014). Production of Common Sour Orange × Carrizo Citrange hybrids using embryo rescue. International Journal of Fruit Science, 14:42-48.
  • Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15:473-497.
  • Murashige, T., & Tucker, P.H. (1969). Growth factor requirements of Citrus tissue culture. Proceedings 1st International Citrus Symposium, 3:1155-1161.
  • Ozsan, M., & Cameron, J.W. (1963). Artificial culture of small Citrus embryos, and evidence against nucellar embryony in highly zygotic varieties. Proceedings of the American Society for Horticultural Science, 82:210–216.
  • Pena, L., Cervera, M., Ghorbel, R., Dominguez, A., Fagoaga, C., Juarez, J., Pina, J.A., & Navarro, L. (2007). Genetic transformation. In: I. A. Khan (Ed.): Citrus, Genetics, Breeding and Biotechnology, CABI Publications, 329-344.
  • Perez-Tornero, O., & Porras, I. (2008). Assessment of polyembryony in lemon: Rescue and in vitro culture of immature embryos. Plant Cell Tissue Organ Culture, 93:173-180.
  • Perez-Tornero, O., Cordoba, D., Moreno, M., Yuste, I., & Porras, I. (2011). Use of classical methods and tools biotecnologiccas in the genetic improvement of the lemon tree: Preliminary results. Hoticultura Global, 296, 14-17.
  • Pestana, M., Varennes, A., Abadia, J., & Faria, E.A. (2005). Differential tolerance to iron deficiency of rootstocks grown in nutrient solutional. Scientia, 104(1):25-36.
  • Raghavan, V. (1980). Embryo culture. In: Perspectives in plant cell and tissue culture. International Review of Cytology, Supplement 11B, 209-240.
  • Rodríguez, M.A., Monter, A.V., Castañeda, G.C., & Velázquez, A.G. (2004). Polyembryony and identification of Volkamerian lemon zygotic and nucellar seedlings using RAPD. Pesquisa Agropecuária Brasileira, 39(6):551-559.
  • Singh, B., Sharma, S., Rani, G., Virk, G.S., Zaidi, A.A., & Nagpal, A. (2006). In vitro flowering in embryogenic cultures of Kinnow mandarin (Citrus nobilis Lour x C. deliciosa Tenora). African Journal of Biotechnology, 5(16):1470-1474.
  • Soni, A., Dubey, A.K., Gupta, A., Sharma, R. M., Awasthi, O.P., Bharadwaj, C., & Sharma, N. (2019). Optimizing embryo age and media for enhancing hybrid seedling recovery in Sour orange (Citrus aurantium) × Sacaton citrumelo (Citrus paradisi × Poncirus trifoliata) crosses through embryo rescue. Plant Breeding, 9 pp.
  • Soost, R.K,. & Cameron, J.W. (1975). Citrus. In: Advances in Fruit Breeding. Purdue University Press, West Lafayette, 507-540.
  • Soost, R.K., & Roose, M.L. (1996). Citrus. In: J. Janick, J. M., Moore (Eds.): Fruit Breeding, Volume 1, Tree and Tropical Fruits, John Wiley, New York, 256-323. ISBN:978-0-471-31014-3.
  • Şeker, M., Tuzcu, O., & Ollitrault, P. (2003). Comparison of nuclear DNA content of Citrus rootstock populations by flow cytometry analysis. Plant Breeding, 122:169-172.
  • Tan, M., Song, J., & Deng, X. (2007). Production of two mandarin x trifoliate orange hybrid populations via embryo rescue with verification by SSR analysis. Euphytica, 157:155-160.
  • Tusa, N., Abbate, L., Ferrante, S., Lucretti, S., & Scarano, M.T. (2002). Identification of zygotic and nucellar seedlings in Citrus interploid crosses by means of isozymes, flow cytometry and ISSR-PCR. Cellular & Molecular Biology Letters, 7(2B):703-708.
  • Tuzcu, Ö. (1978). Turunçgillerde anaç ve sorunları. Çağdaş Tarım Tekniği (3), 31-35 (in Turkish).
  • Viloria, Z., Grosser, J.W., & Bracho, B. (2005). Immature embryo rescue, culture and seedling development of acid Citrus fruit derived from interploid hybridization. Plant Cell, Tissue and Organ Culture, 82, 159-167.
  • Wang, J.F., Chen, Z.G., & Lin, T.X. (1999). Observation on the embryonic development in Citrus after cross pollination. Chinese Developmental Reproductive Biology Society, 8(2):57-63.
  • Xie, K.D., Yuan, D.Y., Wang, W., Xia, Q.M., Wu, X.M., Chen, C.W., Chen, C.L., Grosser, J.W. & Guo, W.W. (2019). Citrus triploid recovery based on 2x × 4x crosses via an optimized embryo rescue approach. Scientia Horticulturae, 252: 104-109.
  • Yun, J.U., Yang, H.B., Jung, Y.H., Yun, S.H., Kim, K.S., Kim, C.S., & Song, K.J. (2007). Identification of zygotic and nucellar mandarin seedling using randomly amplified polymorphic DNA. Horticulture, Environment, and Biotechnology, 48(3):171-175.

Ayrıntılar

Birincil Dil İngilizce
Konular Ziraat Mühendisliği (Diğer)
Bölüm Araştırma Makalesi
Yazarlar

Şenay KURT Bu kişi benim 0000-0002-2921-063X

Fatma KOYUNCU Bu kişi benim 0000-0001-5803-6944

Erken Görünüm Tarihi 18 Ağustos 2023
Yayımlanma Tarihi 27 Kasım 2023
Yayımlandığı Sayı Yıl 2023 Cilt: 40 Sayı: 3

Kaynak Göster

APA KURT, Ş., & KOYUNCU, F. (2023). Improvement of Citrus Rootstock Hybrids Derived by 2x × 2x Intra Crosses with the Aid of Embryo Rescue and Ploidy Detection. Horticultural Studies, 40(3), 72-78. https://doi.org/10.16882/hortis.1343859
AMA KURT Ş, KOYUNCU F. Improvement of Citrus Rootstock Hybrids Derived by 2x × 2x Intra Crosses with the Aid of Embryo Rescue and Ploidy Detection. HortiS. Kasım 2023;40(3):72-78. doi:10.16882/hortis.1343859
Chicago KURT, Şenay, ve Fatma KOYUNCU. “Improvement of Citrus Rootstock Hybrids Derived by 2x × 2x Intra Crosses With the Aid of Embryo Rescue and Ploidy Detection”. Horticultural Studies 40, sy. 3 (Kasım 2023): 72-78. https://doi.org/10.16882/hortis.1343859.
EndNote KURT Ş, KOYUNCU F (01 Kasım 2023) Improvement of Citrus Rootstock Hybrids Derived by 2x × 2x Intra Crosses with the Aid of Embryo Rescue and Ploidy Detection. Horticultural Studies 40 3 72–78.
IEEE Ş. KURT ve F. KOYUNCU, “Improvement of Citrus Rootstock Hybrids Derived by 2x × 2x Intra Crosses with the Aid of Embryo Rescue and Ploidy Detection”, HortiS, c. 40, sy. 3, ss. 72–78, 2023, doi: 10.16882/hortis.1343859.
ISNAD KURT, Şenay - KOYUNCU, Fatma. “Improvement of Citrus Rootstock Hybrids Derived by 2x × 2x Intra Crosses With the Aid of Embryo Rescue and Ploidy Detection”. Horticultural Studies 40/3 (Kasım 2023), 72-78. https://doi.org/10.16882/hortis.1343859.
JAMA KURT Ş, KOYUNCU F. Improvement of Citrus Rootstock Hybrids Derived by 2x × 2x Intra Crosses with the Aid of Embryo Rescue and Ploidy Detection. HortiS. 2023;40:72–78.
MLA KURT, Şenay ve Fatma KOYUNCU. “Improvement of Citrus Rootstock Hybrids Derived by 2x × 2x Intra Crosses With the Aid of Embryo Rescue and Ploidy Detection”. Horticultural Studies, c. 40, sy. 3, 2023, ss. 72-78, doi:10.16882/hortis.1343859.
Vancouver KURT Ş, KOYUNCU F. Improvement of Citrus Rootstock Hybrids Derived by 2x × 2x Intra Crosses with the Aid of Embryo Rescue and Ploidy Detection. HortiS. 2023;40(3):72-8.