Araştırma Makalesi
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Identification of serotonergic 5HT-3B type receptors in broiler’s small intestine

Yıl 2018, Cilt 2, Sayı 1, 8 - 13, 30.04.2018
https://doi.org/10.30704/http-www-jivs-net.404248

Öz

in broiler’s small intestine. Investigation were carried out on isolated smooth muscle of the circular and longitudinal layer of the broilers small intestine (strip dimension 3-4 mm x 2 cm). The muscle strips were placed in an isolated organ bath. The mechanical activity of the preparations were recorded via an isotonic force transducer coupled to a pen recorder. This was done following the addition of serotonin (nonselective 5-HT agonist), 2-Me-5HT (5-HT3B agonist) and Y-25130-hydrochloride (selective 5-HT3B antagonist). The research established a presence of serotonergic 5HT3B type receptors within the smooth musculature of the small intestines of broilers (COBB 500). The 5HT3B type receptors were present in smooth muscles of duodenum, jejunum and ileum, especially in longitudinal smooth muscles since this layer reacted even to low serotonin concentration (10-6). Statistical tests of obtained results showed significant differences (p< 0.001) in responses related to muscle layers, applied concentrations and intestinal parts which were observed. In the light of these findings, we suggest that investigated substances may have considerable physiological and therapeutic implications in disturbed function of small intestine of broiler’s.

Kaynakça

  • Berger, M., Gray, J. A., & Roth, B. L. (2009). The expanded biology of serotonin. Annual Review of Medicine, 60, 355-366.
  • Cirillo, C., Vanden, B. P., & Tack, J. (2011). Role of serotonin in gastrointestinal physiology and pathology. Minerva Endocrinologica, 36, 311-24.
  • Coleman, N. S., Marciani, L., & Blackshaw, E. (2003). Effect of a Novel 5-HT3 Receptor agonist MKC-733 on upper gastrointestinal motility in humans. Alimentary Pharmacology and Therapeutics, 18, 1039-1048.
  • Darmon, M., Awabdh, S., Emerit, M. B., & Masson, J. (2015). Insights into Serotonin Receptor Trafficking: Cell Membrane Targeting and Internalization. Progress in Molecular Biology and Translational Science, 132, 97-126.
  • Galligan, J. J. (2002). Ligand- gated ion channels in the enteric nervous system. Journal of Neurogastroenterology and Motility, 14, 611–623.
  • Glatzle, J., Sternini, C., Robin, C., Tilman, T. Z., Wong, H., Reeve J. R., & Raybould, H. E. (2002). Expression of 5-HT3 receptors in the rat gastrointestinal tract. Journal of Gastroenterology, 123, 217-226.
  • Hannon, J., & Hoyer, D. (2002). Serotonin receptors and systems: endless diversity? Acta Biologica Szegediensiz, 46, 1-12.
  • Hardman, J. G., Limbird, L. E. (2001). Goodman & Gilman’s the pharmacological basis of therapeutics. 10th ed. New York, US: The McGraw-Hill Companies.
  • Hendriks, R., Bornstein, J. C., & Furness, J. B. (1989). Evidence for two types of 5-hydroxytryptamine receptors on secretomotor neurons of the guinea pig ileum. Naunyn-Schimiedeberg’s Archives of Pharmacology, 339, 409–414.
  • Katica, M. (2015). Testing the effects of physiologically and pharmacologically active substances and their antagonists in smooth intestine musculature during the turkey fattening phase. PhD Thesis, University of Sarajevo, Bosnia and Herzegovina.
  • Kato, S. (2013). Role of serotonin 5-HT₃ receptors in intestinal inflammation. Biological and Pharmaceutical Bulletin– Journal, 36, 1406-1409.
  • Kaufman, D. M., & Milstein, M. D. (2013). Neurotransmitters and drug abuse. In D. M. Kaufman, & M. D. Milstein, (Ed), Kaufman's clinical neurology for psychiatrists 7th ed. (pp. 501–525). USA : Elsevier.
  • Mujezinović, I., Ćupić, V., Smajlović, A., & Muminović, M. (2011). Identification of serotonergic (5HT1a-type) receptors in broiler’s small intestine by application of serotonin and its agonists and antagonists. Veterinarski Glasnik, 65, 1-2, 51-59.
  • Muminović, M., Hadžović, S., Abdagić, I., & Smajlović, A. (2000). The Effect of Serotonin on the isolated smooth muscles of the bovine rumen. Veterinaria 49, 65-77.
  • Nagakura, Y., Ito, H., Kamato, T., Nishida, A., & Miyata, K. (1997). Effect of a selective 5-HT3 receptor agonist on gastric motility in fasted and fed dogs. European Journal of Pharmacology, 327, 104–108.
  • Nichols, D. E., & Nichols, C.D. (2008). Serotonin receptors. Chemical Reviews, 108, 1614-1641.
  • Pauwels, P. J. (2003). 5-HT receptors and their ligands. In N. Barker, (Ed.), Tocris Reviews. (pp. 1–12). Bristol, UK: Tocris Cookson.
  • Penkova, N. I., & Nikolova, J. G. (2017). Serotonin and its functions as gastrointestinal hormone. Journal of Gastrointestinal & Digestive System, 7, 1-7.
  • Pytliak, M., Vargová, V., Mechírová, V., & Felšöci, M. (2011). Serotonin Receptors – From Molecular Biology to Clinical Applications. Physiological Research, 60, 15-25.
  • Siegel, G. J., Agranoff, B. W., Albers, R. W., Fisher, S. K., & Uhler M. D. (1999). Basic Neurochemistry: Molecular cellular and medical aspects. 6th (Ed). Philadelphia, US: Lippincott Williams and Wilkins.

Yıl 2018, Cilt 2, Sayı 1, 8 - 13, 30.04.2018
https://doi.org/10.30704/http-www-jivs-net.404248

Öz

Kaynakça

  • Berger, M., Gray, J. A., & Roth, B. L. (2009). The expanded biology of serotonin. Annual Review of Medicine, 60, 355-366.
  • Cirillo, C., Vanden, B. P., & Tack, J. (2011). Role of serotonin in gastrointestinal physiology and pathology. Minerva Endocrinologica, 36, 311-24.
  • Coleman, N. S., Marciani, L., & Blackshaw, E. (2003). Effect of a Novel 5-HT3 Receptor agonist MKC-733 on upper gastrointestinal motility in humans. Alimentary Pharmacology and Therapeutics, 18, 1039-1048.
  • Darmon, M., Awabdh, S., Emerit, M. B., & Masson, J. (2015). Insights into Serotonin Receptor Trafficking: Cell Membrane Targeting and Internalization. Progress in Molecular Biology and Translational Science, 132, 97-126.
  • Galligan, J. J. (2002). Ligand- gated ion channels in the enteric nervous system. Journal of Neurogastroenterology and Motility, 14, 611–623.
  • Glatzle, J., Sternini, C., Robin, C., Tilman, T. Z., Wong, H., Reeve J. R., & Raybould, H. E. (2002). Expression of 5-HT3 receptors in the rat gastrointestinal tract. Journal of Gastroenterology, 123, 217-226.
  • Hannon, J., & Hoyer, D. (2002). Serotonin receptors and systems: endless diversity? Acta Biologica Szegediensiz, 46, 1-12.
  • Hardman, J. G., Limbird, L. E. (2001). Goodman & Gilman’s the pharmacological basis of therapeutics. 10th ed. New York, US: The McGraw-Hill Companies.
  • Hendriks, R., Bornstein, J. C., & Furness, J. B. (1989). Evidence for two types of 5-hydroxytryptamine receptors on secretomotor neurons of the guinea pig ileum. Naunyn-Schimiedeberg’s Archives of Pharmacology, 339, 409–414.
  • Katica, M. (2015). Testing the effects of physiologically and pharmacologically active substances and their antagonists in smooth intestine musculature during the turkey fattening phase. PhD Thesis, University of Sarajevo, Bosnia and Herzegovina.
  • Kato, S. (2013). Role of serotonin 5-HT₃ receptors in intestinal inflammation. Biological and Pharmaceutical Bulletin– Journal, 36, 1406-1409.
  • Kaufman, D. M., & Milstein, M. D. (2013). Neurotransmitters and drug abuse. In D. M. Kaufman, & M. D. Milstein, (Ed), Kaufman's clinical neurology for psychiatrists 7th ed. (pp. 501–525). USA : Elsevier.
  • Mujezinović, I., Ćupić, V., Smajlović, A., & Muminović, M. (2011). Identification of serotonergic (5HT1a-type) receptors in broiler’s small intestine by application of serotonin and its agonists and antagonists. Veterinarski Glasnik, 65, 1-2, 51-59.
  • Muminović, M., Hadžović, S., Abdagić, I., & Smajlović, A. (2000). The Effect of Serotonin on the isolated smooth muscles of the bovine rumen. Veterinaria 49, 65-77.
  • Nagakura, Y., Ito, H., Kamato, T., Nishida, A., & Miyata, K. (1997). Effect of a selective 5-HT3 receptor agonist on gastric motility in fasted and fed dogs. European Journal of Pharmacology, 327, 104–108.
  • Nichols, D. E., & Nichols, C.D. (2008). Serotonin receptors. Chemical Reviews, 108, 1614-1641.
  • Pauwels, P. J. (2003). 5-HT receptors and their ligands. In N. Barker, (Ed.), Tocris Reviews. (pp. 1–12). Bristol, UK: Tocris Cookson.
  • Penkova, N. I., & Nikolova, J. G. (2017). Serotonin and its functions as gastrointestinal hormone. Journal of Gastrointestinal & Digestive System, 7, 1-7.
  • Pytliak, M., Vargová, V., Mechírová, V., & Felšöci, M. (2011). Serotonin Receptors – From Molecular Biology to Clinical Applications. Physiological Research, 60, 15-25.
  • Siegel, G. J., Agranoff, B. W., Albers, R. W., Fisher, S. K., & Uhler M. D. (1999). Basic Neurochemistry: Molecular cellular and medical aspects. 6th (Ed). Philadelphia, US: Lippincott Williams and Wilkins.

Ayrıntılar

Birincil Dil İngilizce
Konular Veteriner Hekimlik
Bölüm Araştırma Makaleleri
Yazarlar

İndira MUJEZİNOVİĆ>
University of Sarajevo, Veterinary Faculty, Department of Pharmacology
Bosnia and Herzegovina


Muhamed KATİCA> (Sorumlu Yazar)
University of Sarajevo, Veterinary Faculty, Department of Pathophysiology
Bosnia and Herzegovina


Ahmed SMAJLOVİĆ>
University of Sarajevo, Veterinary Faculty, Department of Pharmacology
Bosnia and Herzegovina


Nedžad GRADAŠČEVİĆ Bu kişi benim
University of Sarajevo, Veterinary Faculty, Department of Radiobiology with Radiation Hygiene, Biophysics & Environmental Protection
Bosnia and Herzegovina


Behija DUKİĆ>
University of Sarajevo, Veterinary Faculty, Department of Microbiology, Immunology and Infectious Diseases
Bosnia and Herzegovina

Yayımlanma Tarihi 30 Nisan 2018
Yayınlandığı Sayı Yıl 2018, Cilt 2, Sayı 1

Kaynak Göster

APA Mujezinović, İ. , Katica, M. , Smajlović, A. , Gradaščević, N. & Dukić, B. (2018). Identification of serotonergic 5HT-3B type receptors in broiler’s small intestine . Journal of Istanbul Veterinary Sciences , 2 (1) , 8-13 . DOI: 10.30704/http-www-jivs-net.404248

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