Authentication of Medicinal Chemicals in Traditional Herbal Products (Jamu) by UV-Vis Spectrophotometry

Abstract

Traditional medicine is becoming very popular as an alternative treatment that can minimize side effects. However, it is profitable for manufacturers who are not responsible for adding medicinal chemicals (BKO) to traditional herbal products. The samples used in this study were traditional herbal medicine coded A1, A2; B1, B2; C1, C2; D1, D2; E1, E2; and F1, F2, which were analyzed qualitatively and quantitatively using thin-layer chromatography (TLC) and UV-Visible spectrophotometry. Based on the results obtained from the analysis using the TLC method, the herbal samples with code A1 and A2 had a staining profile, Rf value, and chromatographic peak that were almost the same as the comparison standard for sodium metamizole with levels of 51.99% (A1) and 53.06% (A2). The herbal medicine samples with codes B1, B2, and C1, C2 had stain profiles, Rf values in TLC, and spectrophotometric peaks that were almost the same as the comparison standard for paracetamol with paracetamol levels contained in the “Jamu” samples of 81.31% (B1), 81.66% (B2), 89.59% (C1) and 81.62% (C2). The results obtained from this study show that the samples of traditional herbal products with codes A1 and A2 contain sodium metamizole with levels of >50%. Samples of traditional herbal products B1, B2, C1, and C2 contain paracetamol with paracetamol levels contained > 50%, while the traditional herbal medicine samples with codes D1, D2, E1, E2, F1, and F2 have been identified as containing no medicinal chemicals in the traditional herbal medicine samples.

Keywords

• traditional herbal medicine
• jamu
• medicinal chemicals
• UV-Vis spectrophotometry

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