Systematic Scrutinization of Vital factors for the Development of Efficient Cisplatin-Quercetin Loaded Bionanomicelles

Hardik Rana; Neha Sisodia; Mansi Dholakia; Vaishali Gandhi

doi:10.52794/hujpharm.1298173

EN TR

Systematic Scrutinization of Vital factors for the Development of Efficient Cisplatin-Quercetin Loaded Bionanomicelles

Abstract

The present work aims to optimize and assess the Cisplatin (CIS) and Quercetin (QCT)-loaded biodegradable polymeric nanomicelles (PNM). The development of a quantitative method for the estimation of CIS and QCT in pharmaceutical dosage form was another objective. The aluminum plates coated with silica gel F254 used for separation of both the drugs employing Toluene: Methanol: Ethyl acetate: DMF: Triethylamine (5:0.5:3.5:1:1 drop % v/v/v/v/v) as mobile phase. Results of the validation parameter indicate that the developed method was precise, accurate, and robust. CIS and QCT-loaded PNM formulated using solvent evaporation technique employing poly lactic-co-glycolic acid (PLGA) 50:50. The Quality by Design (QbD) was accomplished to identify the critical manufacturing attributes and critical process parameters. Optimization of the formulation was performed by central composite design using particle size and % encapsulation efficiency as dependent variables. The amount of PLGA and Span selected as independent variables. Statistically substantial variables identified using regression analysis and analysis of variance. A diffusion study revealed that optimized nanomicelles were capable to sustain the drug release up to 8h. Zeta sizer, TEM confirmed the stability and nano-sized nanoparticles. CIS-QCT PNM was found to be an alternate route to systemic treatment.

Keywords

Systematic Scrutinization of Vital factors for the Development of Efficient Cisplatin-Quercetin Loaded Bionanomicelles

Abstract

The present work aims to optimize and assess the Cisplatin (CIS) and Quercetin (QCT)-loaded biodegradable polymeric nanomicelles (PNM). The development of a quantitative method for the estimation of CIS and QCT in pharmaceutical dosage form was another objective. The aluminum plates coated with silica gel F254 used for separation of both the drugs employing Toluene: Methanol: Ethyl acetate: DMF: Triethylamine (5:0.5:3.5:1:1 drop % v/v/v/v/v) as mobile phase. Results of the validation parameter indicate that the developed method was precise, accurate, and robust. CIS and QCT-loaded PNM formulated using solvent evaporation technique employing poly lactic-co-glycolic acid (PLGA) 50:50. The Quality by Design (QbD) was accomplished to identify the critical manufacturing attributes and critical process parameters. Optimization of the formulation was performed by central composite design using particle size and % encapsulation efficiency as dependent variables. The amount of PLGA and Span selected as independent variables. Statistically substantial variables identified using regression analysis and analysis of variance. A diffusion study revealed that optimized nanomicelles were capable to sustain the drug release up to 8h. Zeta sizer, TEM confirmed the stability and nano-sized nanoparticles. CIS-QCT PNM was found to be an alternate route to systemic treatment.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmacology and Pharmaceutical Sciences

Journal Section

Research Article

Authors

Hardik Rana ^*
0000-0002-2159-7665
India

Neha Sisodia
0009-0000-4522-5885
India

Mansi Dholakia This is me
0000-0001-8141-6013
India

Vaishali Gandhi
0000-0001-6332-7703
India

Publication Date

June 1, 2024

Submission Date

May 17, 2023

Acceptance Date

May 18, 2024

Published in Issue

Year 2024 Volume: 44 Number: 2

DOI

https://doi.org/10.52794/hujpharm.1298173

IZ

https://izlik.org/JA57BJ76MF

Cite

RIS / Bibtex

APA

Rana, H., Sisodia, N., Dholakia, M., & Gandhi, V. (2024). Systematic Scrutinization of Vital factors for the Development of Efficient Cisplatin-Quercetin Loaded Bionanomicelles. Hacettepe University Journal of the Faculty of Pharmacy, 44(2), 92-107. https://doi.org/10.52794/hujpharm.1298173

AMA

1.Rana H, Sisodia N, Dholakia M, Gandhi V. Systematic Scrutinization of Vital factors for the Development of Efficient Cisplatin-Quercetin Loaded Bionanomicelles. HUJPHARM. 2024;44(2):92-107. doi:10.52794/hujpharm.1298173

Chicago

Rana, Hardik, Neha Sisodia, Mansi Dholakia, and Vaishali Gandhi. 2024. “Systematic Scrutinization of Vital Factors for the Development of Efficient Cisplatin-Quercetin Loaded Bionanomicelles”. Hacettepe University Journal of the Faculty of Pharmacy 44 (2): 92-107. https://doi.org/10.52794/hujpharm.1298173.

EndNote

Rana H, Sisodia N, Dholakia M, Gandhi V (June 1, 2024) Systematic Scrutinization of Vital factors for the Development of Efficient Cisplatin-Quercetin Loaded Bionanomicelles. Hacettepe University Journal of the Faculty of Pharmacy 44 2 92–107.

IEEE

[1]H. Rana, N. Sisodia, M. Dholakia, and V. Gandhi, “Systematic Scrutinization of Vital factors for the Development of Efficient Cisplatin-Quercetin Loaded Bionanomicelles”, HUJPHARM, vol. 44, no. 2, pp. 92–107, June 2024, doi: 10.52794/hujpharm.1298173.

ISNAD

Rana, Hardik - Sisodia, Neha - Dholakia, Mansi - Gandhi, Vaishali. “Systematic Scrutinization of Vital Factors for the Development of Efficient Cisplatin-Quercetin Loaded Bionanomicelles”. Hacettepe University Journal of the Faculty of Pharmacy 44/2 (June 1, 2024): 92-107. https://doi.org/10.52794/hujpharm.1298173.

JAMA

1.Rana H, Sisodia N, Dholakia M, Gandhi V. Systematic Scrutinization of Vital factors for the Development of Efficient Cisplatin-Quercetin Loaded Bionanomicelles. HUJPHARM. 2024;44:92–107.

MLA

Rana, Hardik, et al. “Systematic Scrutinization of Vital Factors for the Development of Efficient Cisplatin-Quercetin Loaded Bionanomicelles”. Hacettepe University Journal of the Faculty of Pharmacy, vol. 44, no. 2, June 2024, pp. 92-107, doi:10.52794/hujpharm.1298173.

Vancouver

1.Hardik Rana, Neha Sisodia, Mansi Dholakia, Vaishali Gandhi. Systematic Scrutinization of Vital factors for the Development of Efficient Cisplatin-Quercetin Loaded Bionanomicelles. HUJPHARM. 2024 Jun. 1;44(2):92-107. doi:10.52794/hujpharm.1298173