Antioksidan aktiviteler, A549 hücre hatlarına karşı sitotoksisite aktivitesi ve Pseudodrynaria coronans (Wall. Ex Mett.) Ching rizomunun HPTLC parmak izi

Abstract

Bitkilerden elde edilen fitokimyasallar, bitkiler tarafından kendi kullanımı için sentezlenen ikincil metabolitleri içerir. Çeşitli hastalıkların önlenmesi ve tedavisi için bitki fitokonstitülerinin araştırılması, yeni ilaç varlığının keşfedilmesine yol açar. Bitki fitokonstitülerinin uygun şekilde doğrulanmasının kullanılması. Bu çalışmada, Pseudodrynaria coronans (Wall. Ex Mett.) Ching köksapının metanol özütü, A549 hücre hatlarına karşı antioksidan ve sitotoksisite çalışmaları için incelenmiştir. Metanol özütünün HPTLC parmak izi analizi, geliştirilmiş metanol: kloroform: formik asit (10: 0,5: 0,5 v/v/v) çözücüsü kullanılarak gerçekleştirilmiştir. DPPH serbest radikal temizleme aktivitesi ve indirgeyici güç testi, özütün sırasıyla 286,7 ve 181,36 µg/mL IC50 değerlerine sahip dikkate değer miktarda antioksidan aktivite içerdiğini göstermiştir. MTT testi için 88,64 µg/mL'lik IC50 değeri ayrıca bitkilerin A549 hücre hatları üzerinde önemli miktarda sitotoksisite etkisi içerdiğini ortaya koydu ve bu daha fazla araştırılmalıdır. HPTLC parmak izinin geliştirilmesi, steroidler, flavonoidler, tanenler, saponinler, triterpenoidler, amino asitler ve karbonhidratlar olan farklı fitokimyasal sınıflarının varlığını; alkaloidler, glikozitler, indirgeyici şeker, yağlar ve sabit yağların yokluğunu gösterdi ve bitki materyalinin kimlik doğrulaması için bir kalite kontrol aracı sağladı.

Keywords

• Fitokimyasallar
• Antioksidan
• Sitotoksisite
• HPTLC
• A549.

Antioxidant activities, cytotoxicity activity against A549 cell lines and HPTLC fingerprinting of Pseudodrynaria coronans (Wall. Ex Mett.) Ching rhizome

Abstract

Phytochemicals obtained from the plants include the secondary metabolite synthesized by the plants for its own utilization. Exploration of plants phytoconstituents for the prevention and treatment of various disease leads to the discovery of new drug entity. Utilization of proper authentication of the plant phytoconstituents. In this study, methanolic extract of rhizome of Pseudodrynaria coronans (Wall. Ex Mett.) Ching was studied for its antioxidant and cytotoxicity studies against A549 cell lines. HPTLC fingerprint analysis of the methanolic extract was performed using the developed solvent of methanol:chloroform: formic acid (10:0.5:0.5 v/v/v). DPPH free radicals scavenging activity and reducing power assay showed that the extract contains a remarkable amount of antioxidant activity possessing IC50 values of 286.7 and 181.36 μg/mL respectively. IC50 value of 88.64 μg/mL for MTT assay also revealed that the plants also contain significant amount of cytotoxicity action on A549 cell lines, which needs to be explored further. The development of the HPTLC fingerprint indicated the presence of different classes of phytochemicals, which are steroids, flavonoids, tannins, saponins, triterpenoids, amino acids, and carbohydrates; the absence of alkaloids, glycosides, reducing sugar, fats and fixed oils, and provided a quality control tool for the authentication of plant material.

Keywords

• Phytochemicals
• Antioxidant
• Cytotoxicity
• HPTLC
• A549.

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