A Comparative Study on Some Biological Activities of Different Parts of Vincetoxicum hirundinaria

Year 2025, Volume: 45 Issue: 4 , 345 - 352 , 01.12.2025

Didem Aksu , Sevda Güzel Kara

https://doi.org/10.52794/hujpharm.1699849

https://izlik.org/JA66JK97JH

Abstract

Vincetoxicum hirundinaria Medik. (Apocynaceae) is commonly known as white swallow-wort. Numerous medicinal uses of the species in different countries have been documented in the literature. This research focused on analyzing ethanol-based extracts from various anatomical parts of V. hirundinaria, including flowers, leaves, seeds, and seed pods, to assess their phenolic and flavonoid compound levels, antioxidant activities, and cytotoxic profile. Quantitative assessment of total phenolics and flavonoids was carried out using the Folin–Ciocalteu procedure and the AlCl₃ colorimetric assay, in that order. For antioxidant activity the DPPH radical scavenging assay was used. Total phenolics and flavonoids were detected within the ranges of 8.26–5.34 mg GAE/g and 5.36–2.40 mg QE/g, respectively. The flower extract showed the greatest antioxidant performance, with 75.2% activity and an ascorbic acid equivalent of 13.7 mM per gram of sample. Furthermore, cytotoxicity was tested on HaCaT cell lines using the MTT assay. In all experiments involving plant extracts and at all tested concentrations (0.25-1 mg/mL) no toxic effects were observed on HaCaT cells. These findings indicate the plant's potential for cosmetic, dermatological, and pharmaceutical use. This is the first comparative study performed on ethanol extracts of different parts of V. hirundinaria.

Keywords

DPPH , cytotoxicity , total phenolic content , total flavonoid content , Vincetoxicum hirundinaria

Supporting Institution

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Project Number

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Thanks

The authors would like to thank Prof. Dr. Ahmet KAHRAMAN for the identification of the plant sample.

References

• 1. Liede-Schumann S, Khanum R, Mumtaz AS, Gherghel I, Pahlevani A. Going west-a subtropical lineage (Vincetoxicum, Apocynaceae: Asclepiadoideae) expanding into Europe. Mol Phylogenet Evol. 2016;94:436-446. https://doi.org/10.1016/j.ympev.2015.09.021
• 2. Prameela A, Krishnasamy T, Saraswathy SD. Ethnobotany, phylogeny, phytochemistry and pharmacological applications of genus Vincetoxicum (Apocynaceae). Discov. Plants. 2024;1:32. https://doi.org/10.1007/s44372-024-00031-0
• 3. Khan A, Shah AH, Ali N, Majid A, Shah SH. Conservation strategy, phytochemical diversity, and genetic consistency assessment of in vitro propagated perennial herb Vincetoxicum hirundinaria Medik. In Vitro Cell.Dev.Biol.-Plant. 2023;59:258-274. https://doi.org/10.1007/s11627-023-10347-3
• 4. Jovaišaitė J, Jūrienė L, Pukalskas A, Baranauskienė R, Ragažinskienė O, Venskutonis PR. Recovery of lipophilic compounds of Vincetoxicum species by supercritical carbon dioxide extraction. J. Supercrit. Fluids. 2024;206:106159. https://doi.org/10.1016/j.supflu.2023.106159
• 5. Šliumpaitė I, Murkovic M, Zeb A, Venskutonisa PR. Antioxidant properties and phenolic composition of swallowwort (Vincetoxicum lutea L.) leaves. Ind Crops Prod. 2013;45:74-82. https://doi.org/10.1016/j.indcrop.2012.11.036
• 6. Zaidi MA, Crow JSA. Biologically active traditional medicinal herbs from Balochistan, Pakistan. J Ethnopharmacol. 2005;96(1-2):331-334. https://doi.org/10.1016/j.jep.2004.07.023
• 7. Gairola S, Sharma J, Bedi YS. A cross-cultural analysis of Jammu, Kashmir and Ladakh (India) medicinal plant use. J Ethnopharmacol. 2014;155(2):925-986. https://doi.org/10.1016/j.jep.2014.06.029
• 8. Bibi T, Ahmad M, Tareen NM, Jabeen R, Sultana S, Zafar M, et al. The endemic medicinal plants of Northern Balochistan, Pakistan and their uses in traditional medicine. J Ethnopharmacol. 2015;173:1-10. https://doi.org/10.1016/j.jep.2015.06.050
• 9. Zahra Z, Khan MR, Shah SA, Maryam S, Majid M, Younis T, et al. Vincetoxicum arnottianum ameliorate inflammation by suppressing oxidative stress and pro-inflammatory mediators in rat. J Ethnopharmacol. 2020;252:112565. https://doi.org/10.1016/j.jep.2020.112565
• 10. Browicz K. Vincetoxicum N.M. Wolf. In: Davis PH, eds. Flora of Turkey and the East Aegean Islands. Edinburgh, Scotland; 1978. p. 163-173
• 11. Lavault M, Richomme P, Bruneton J. Acetophenones and new pregnane glycosides from the roots of Vincetoxicum hirundinaria. Fitoterapia. 1999;70(2):216-220. https://doi.org/10.1016/S0367-326X(99)00023-4
• 12. Kalske A, Leimu R, Scheepens JF, Mutikainen P. Spatiotemporal variation in local adaptation of a specialist insect herbivore to its long-lived host plant. Evolution. 2016;70(9):2110-2122. https://doi.org/10.1111/evo.13013
• 13. Slapšytė G, Dedonytė V, Adomėnienė A, Lazutka JR, Kazlauskaitė J, Ragažinskienė O, et al. Genotoxic properties of Betonica officinalis, Gratiola officinalis, Vincetoxicum luteum and Vincetoxicum hirundinaria extracts. Food Chem Toxicol. 2019;134:110815. https://doi.org/10.1016/j.fct.2019.110815
• 14. Baytop T. Türkiye’de Bitkiler ile Tedavi Geçmişte ve Bugün. İlaveli 2. Baskı; İstanbul Üniversitesi Eczacılık Fakültesi, Nobel Tıp Kitap Evleri, İstanbul, Türkiye 1984.
• 15. Coassini-Lokar L, Poldini L. Herbal remedies in the traditional medicine of the Venezia Giulia Region (North East Italy). J Ethnopharmacol. 1988;22(3):231-279. https://doi.org/10.1016/0378-8741(88)90238-3
• 16. Lavault M, Richomme P, Bruneton J. New phenanthroindolizidine N-oxides alkaloids isolated from Vincetoxicum hirundinaria Medic. Pharm. Acta Helv. 1994;68(4):225-227. https://doi.org/10.1016/0031-6865(94)90052-3
• 17. Bonet MA, Parada M, Selga A, Vallès J. Studies on pharmaceutical ethnobotany in the regions of L’Alt Empordà and Les Guilleries (Catalonia, Iberian Peninsula). J Ethnopharmacol. 1999;68(1-3):145-168. https://doi.org/10.1016/S0378-8741(99)00083-5
• 18. Rigat M, Vallesè J, D’Ambrosio U, Gras A, Iglésias J: Plants with topical uses in the Ripollès district (Pyrenees, Catalonia, Iberian Peninsula): Ethnobotanical survey and pharmacological validation in the literature. J Ethnopharmacol. 2015;164:162-179. https://doi.org/10.1016/j.jep.2015.01.055
• 19. Bizim Bitkiler, Nezahat Gökyiğit Botanik Bahçesi. 2025. [cited April 2023] Available from: https://bizimbitkiler.org.tr/yeni/demos/technical/
• 20. DiTommaso A, Lawlor FM, Darbyshire SJ. The biology of invasive alien plants in Canada 2. Cynanchum rossicum (Kleopow) Borhidi (=Vincetoxicum rossicum (Kleopow) Barbar.) and Cynanchum louseae (L.) Kartesz & Gandhi (=Vincetoxicum nigrum (L.) Moench). Can. J. Plant Sci. 2005;85(1):243-263. https://doi.org/10.4141/P03-056
• 21. Laukkanen L, Leimu R, Muola A, Lilley M, Salminen JP, Mutikainen P. Plant chemistry and local adaptation of a specialized folivore. PLoS One. 2012;7(5):1-8. https://doi.org/10.1371/journal.pone.0038225
• 22. Staerk D, Christensen J, Lemmich E, Duus JO, Olsen CE, Jaroszewski JW. Cytotoxic activity of some phenanthroindolizidine N-oxide alkaloids from Cynanchum vincetoxicum. J. Nat. Prod. 2000;63(11):1584-1586. https://doi.org/10.1021/np0003443
• 23. Staerk D, Lykkeberg AL, Christensen J, Budnik BA, Abe F, Jaroszewski JW. In vitro cytotoxic activity of phenantroindolizidine alkaloids from Cynanchum vincetoxicum and Tylophora tanakae against drug-sensitive and multidrug-resistant cancer cells. J. Nat. Prod. 2002;65:1299-1302. https://doi.org/10.1021/np0106384
• 24. Nowak R, Kisiel W. Hancokinol from Vincetoxicum officinale. Fitoterapia. 2000;71(5):584-586. https://doi.org/10.1016/S0367-326X(00)00157-X
• 25. Öksüz Z, Güzel Kara S. Investigation on some biological activities of different parts of Vincetoxicum hirundinaria Medik. Hacettepe University Journal of the Faculty of Pharmacy. 2023;43(2):142-149. https://doi.org/10.52794/hujpharm.1128462
• 26. Pavela R. Larvicidal effects of some Euro-Asiatic plants against Culex quinquefasciatus Say larvae (Diptera: Culicidae). Parasitol Res. 2009;105:887-892. https://doi.org/10.1007/s00436-009-1511-0
• 27. Pavela R. Antifeedant activity of plant extracts on Leptinotarsa decemlineata Say. and Spodoptera littoralis Bois. larvae. Ind Crops Prod. 2010;32(3):213-219. https://doi.org/10.1016/j.indcrop.2010.04.010
• 28. Wronski S, Dannenmaier J, Schild S, Macke O, Müller L, Burmeister Y, et al. Engystol reduces onset of experimental respiratory syncytial virus-induced respiratory inflammation in mice by modulating macrophage phagocytic capacity. PLoS One. 2018;13(4):e0195822. https://doi.org/10.1371/journal.pone.0195822
• 29. Saxena S N, Saxena P, Rathore S S, Sharma L K, Saxena R, Barnwal P. Effect of cryogenic grinding on phenolic compounds and antioxidant properties of fenugreek (Trigonella foenum-graecum L.) seed extract. Journal of Spices and Aromatic Crops. 2016;25(1):73-78.
• 30. Kocazorbaz E K, Un R N, Erdag A, Zihnioglu F. Inhibitory effects of some Bryophytes on glutathione-s-transferase. Curr. Enzyme Inhib. 2017;13(1):34-40. https://doi.org/10.2174/1573408012666160628081431
• 31. Pavithra K, Vadivukkarasi S. Evaluation of free radical scavenging activity of various extracts of leaves from Kedrostis foetidissima (Jacq.) Cogn. Food Sci. Hum. Wellness. 2015;4(1):42-46. https://doi.org/10.1016/j.fshw.2015.02.001
• 32. Baudouin C, Charveron M, Tarroux R, Gall Y. Environmental pollutants and skin cancer. Cell Biol Toxicol. 2002;18(5):341-348. https://doi.org/10.1023/A:1019540316060
• 33. Biological evaluation of medical devices. Part 5: tests for in vitro cytotoxicity. Geneva: International Organization for Standardization (ISO), 2009
• 34. Güzel Kara S, Aksu D, Öksüz Z, Uras Güngör ŞS. Study on chemical content and the antioxidant, antimicrobial and antibiofilm potential of Vincetoxicum hirundinaria seeds growing wild in Turkey. Plant Biosystems-An International Journal Dealing with all Aspects of Plant Biology. 2025. https://doi.org/10.1080/11263504.2025.2519076
• 35. do Nascimento Pedrosa T, Catarino CM, Pennacchi PC, de Assis SR, Gimenes F, Consolaro MEL, et al. A new reconstructed human epidermis for in vitro skin irritation testing. Toxicol In Vitro. 2017;42:31-37. https://doi.org/10.1016/j.tiv.2017.03.010
• 36. Şenkal S, Burukçu D, Hayal TB, Kiratli B, Şi̇şli̇ HB, Sağraç D. et al. 3D culture of HaCaT Keratinocyte cell lines as an in vitro toxicity model. Trakya University Journal of Natural Sciences. 2022;23(2):211-220. https://doi.org/10.23902/trkjnat.1158811