Phytochemical Investigation and Antibacterial Activity of Longan Peel Extracts Using Green Solvents

Shu Xian Chong; Lee Huang Chia; Wen Xin Lim

doi:10.52794/hujpharm.1768487

Phytochemical Investigation and Antibacterial Activity of Longan Peel Extracts Using Green Solvents

Abstract

Dimocarpus longan (longan) is a tropical fruit commonly cultivated in China, Vietnam, and Thailand. The pulp, seed, flower, twig, and pericarp of longan have been extensively studied but the peel is often discarded as waste and remain underexplored. The extracts of longan peel were prepared using green solvents (methanol, ethanol, and ethyl acetate). The phytochemical profile of the extracts was screened by the thin layer chromatography (TLC) analysis. The antibacterial activity of the extracts was determined by the disk diffusion method and Minimum Inhibitory Concentration (MIC). The TLC analysis showed that the ethyl acetate extract possessed the most phytochemicals (phenolic compounds, flavonoids, tannins and steroids) compared to the methanol and ethanol extracts. All the extracts exhibited comparable antibacterial activity against Bacillus cereus (B. cereus) where the diameters of inhibition zones were ~ 9.01-9.77 mm. The MIC values for methanol and ethanol extracts were 1 mg/mL, however, the MIC value of the ethyl acetate extract could not be determined because the high water content in the broth caused sample precipitation. In summary, the longan peel extracts show potential as natural antibacterial agents against B. cereus.

Keywords

References

1. Zeng S, Wang K, Liu X, Hu Z, Zhao L. Potential of longan (Dimocarpus longan Lour.) in functional food: A review of molecular mechanism-directing health benefit properties, Food Chem. 2024;437(1):137812. https://doi.org/10.1016/j.foodchem.2023.137812
2. Lin Y, Chen Y, Zheng Y, Zhang H, Lin M, Wang H, et al. Energy and respiratory metabolism participate in dicyclohexylcarbodiimide and disodium succinate-mediated the alteration of energy status modulating pericarp browning of fresh longan. Postharvest Biol Technol. 2024;213:112959. https://doi.org/10.1016/j.postharvbio.2024.112959
3. Lin P, Kan KW, Chen JH, Lin YK, Lin YH, Lin YH, et al. Investigation of the synergistic effect of brown sugar, longan, ginger, and jujube (brown sugar longan ginger tea) on antioxidation and anti-inflammation in in vitro models. Evid Based Complement Alternat Med. 2020;2020 (1):3596085. https://doi:10.1155/2020/3596085.eCollection 2020.
4. Kunworarath N, Rangkadilok N, Suriyo T, Thiantanawat A, Satayavivad J. Longan (Dimocarpus longan Lour.) inhibits lipopolysaccharide-stimulated nitric oxide production in macrophages by suppressing NF-κB and AP-1 signaling pathways. J Ethnopharmacol. 2016;179:156–161. https://doi.org/10.1016/j.jep.2015.12.044
5. Lin CC, Chung YC, Hsu CP. Potential roles of longan flower and seed extracts for anti-cancer. World J Exp Med. 2012;2(4):78–85. https://doi.org/10.5493/wjem.v2.i4.78
6. Zhu X, Wang H, Sun J, Yang B, Duan X, Jiang Y. Pericarp and seed of litchi and longan fruits: Constituent, extraction, bioactive activity, and potential utilization. J Zhejiang Univ Sci B. 2019;20(6):503–512. https://doi.org/10.1631/jzus.B1900161
7. Li H, Lei T, Zhang J, Yan Y, Wang N, Song C, et al. Longan (Dimocarpus longan Lour.) aril ameliorates cognitive impairment in AD mice induced by combination of D-Gal/AlCl3 and an irregular diet via RAS/MEK/ERK signaling pathway. J Ethnopharmacol. 2021;267:113612. https://doi.org/10.1016/j.jep.2020.113612
8. Wang Z, Gao X, Li W, Tan S, Zheng, Q. Phenolic content, antioxidant capacity, and α-amylase and α-glucosidase inhibitory activities of Dimocarpus longan Lour. Food Sci Biotechnol. 2020;29(5);683–692. https://doi.org/10.1007/s10068-019-00708-3

9. Tiu SY, Chong SX. Phytochemical screening and antioxidant activity evaluation of mandarin peels extracts. Lat Am J Pharm. 2024;43(10):1–8.
10. Sonam M, Singh RP, Pooja S. Phytochemical screening and TLC profiling of various extracts of Reinwardtia indica. Int J Pharmacogn Pharm Res. 2017;9(4):523–527. https://doi.org/10.25258/phyto.v9i2.8125
11. Syarifah A, Retnowati R, Soebiantoro S. Characterization of secondary metabolites profile of flavonoid from Salam leaves (Eugenia polyantha) using TLC and UV- spectrophotometry. Pharm Sci Res. 2019;6(3):155–163. https://doi.org/10.7454/psr.v6i3.4219
12. Al-Deen AMT, Alhaidari SAA, Al-Kaf AG, Al-Hadi FA, Al Mahbashi A. Phytochemical screening and thin layer chromatographic of Prunus dulcis (almond) medicinal plant leaves used in folk medicine for treatment of wounds and burns in Hufash District Al Mahweet Governorate–Yemen. Univers J Pharm Res. 2019;4(2):19–23. https://doi.org/10.22270/ujpr.v4i2.252
13. Wutsqa Y, Suratman, Sari S. Detection of terpenoids and steroids in Lindsaea obtusa with thin layer chromatography. Asian J Nat Prod Biochem. 2021;19(2):66–69. https://doi.org/10.13057/biofar/f190204
14. Leela K, Anchana Devi C. Isolation, purification and application of secondary metabolites from lichen Parmelia perlata. Biosci Biotech Res Asia. 2017;14(4):1413–1428. https://doi: http://dx.doi.org/10.13005/bbra/2587
15. Wahab O, Ayodele A, Moody J. TLC phytochemical screening in some Nigerian loranthaceae. J Pharmacognosy Phytother. 2010;2:64–70. http://dx.doi.org/10.13005/bbra/2587
16. Dalimunthe A, Muhammad M, Waruwu S, Rafi M, Kaban V, Satria D. Phytochemicals and proximate analysis of Litsea cubeba Lour. barks. IOP Conf Ser: Earth Environ Sci. 2023;1188:012012. https://doi.10.1088/1755-1315/1188/1/012012
17. Hudzicki, J. Kirby-Bauer disk diffusion susceptibility test protocol. American Society for Microbiology, 2009.
18. Sotthibandhu DS, Visutthi M. Efficacy of Thai plant extracts for antibacterial and anti-biofilm activities against pathogenic bacteria. Antibiotics. 2021;10:1470. https://doi.org/10.3390/antibiotics10121470
19. Al Ubeed HMS, Bhuyan DJ, Alsherbiny MA, Basu A, Vuong QV. A comprehensive review on the techniques for extraction of bioactive compounds from medicinal cannabis. Mol. 2022;27(3):604. https://doi.org/10.3390/molecules27030604
20. Nandiyanto ABD, Oktiani R, Ragadhita R. How to read and interpret FTIR spectroscope of organic material. I JoST. 2019;4 (1):97–118. https://doi.org/10.17509/ijost.v4i1.15806
21. Tseng HC, Wu WT, Huang HS, Wu MC. Antimicrobial activities of various fractions of Longan (Dimocarpus longan Lour. Fen Ke) seed extract. Int J Food Sci Nutr. 2014;65 (5),589–593. https://doi.org/10.3109/09637486.2014.886181
22. Zhang X, Guo S, Ho CT, Bai N. Phytochemical constituents and biological activities of Longan (Dimocarpus Longan Lour.) fruit: A Review. Food Science and Human Wellness. 2020;9 (2):95–102. https://doi.org/10.1016/j.fshw.2020.03.001
23. Garg J, Ghoshal G, Bhadada SK, Katare OP. Derivatisation mechanistic-guided identification of phytoconstituents of different extracts of Cissus quadrangularis by TLC and standardization by HPTLC. Phytomed Plus. 2024;4(3):100601. https://doi.org/10.1016/j.phyplu.2024.100601
24. Zagoskina NV, Zubova MY, Nechaeva TL, Kazantseva VV, Goncharuk EA, Katanskaya VM, et al.. Polyphenols in plants: structure, biosynthesis, abiotic stress regulation, and practical applications (Review). Int J Mol Sci. 2023;24(18):13874. https://doi.org/10.3390/ijms241813874
25. Bai X, Pan R, Li M, Li X, Zhang H. HPLC profile of Longan (Cv. Shixia) pericarp-sourced phenolics and their antioxidant and cytotoxic effects. Molecules. 2019;24 (3):619. https://doi.org/10.3390/molecules24030619
26. Sun J, Shi J, Jiang Y, Xue SJ, Wei X. Identification of two polyphenolic compounds with antioxidant activities in longan pericarp tissues. J Agric Food Chem. 2007;55(14):5864–5868. https://doi.org/10.1021/jf070839z
27. Zhang R, Khan SA, Lin Y, Guo D, Pan X, Liu L, et al.. Phenolic profiles and cellular antioxidant activity of longan pulp of 24 representative chinese cultivars. Int J Food Prop. 2018;21(1):746–759. https://doi.org/10.1080/10942912.2018.1425705
28. CLSI. Performance standards for antimicrobial susceptibility testing. 30th ed. CLSI Supplement M100. Wayne, PA: Clinical and Laboratory Standards Institute; 2020.
29. Batt CA. Bacillus | Bacillus cereus. in Encyclopedia of Food Microbiology. 2nd Ed. United States: Academic Press, 2014;124–128.
30. Mohammadi B, Gorkina N, A. Smith S. Pathogenicity, toxin production, control and detection of Bacillus cereus [Internet]. Foodborne pathogens - Recent advances in control and detection. IntechOpen; 2023. Available from: http://dx.doi.org/10.5772/intechopen.104228
31. Wang M, Chen T, Wang Q, Shi Y. Antioxidant, bacteriostatic and preservative effects of extractable condensed tannins isolated from longan pericarps and seeds. Plants. 2023;12(3):512. https://doi.org/10.3390/plants12030512
32. Chollakup R, Kongtud W, Sukatta U, Premchookiat M, Piriyasatits K, Nimitkeatkai H, Jarerat A. Eco-friendly rice straw paper coated with longan (Dimocarpus longan) peel extract as bio-based and antibacterial packaging. Polymers. 2021;13(18):3096. https://doi.org/10.3390/polym13183096
33. Tseng H-C, Wu W-T, Huang H-S, Wu M-C. Antimicrobial activities of various fractions of longan (Dimocarpus longan Lour. Fen Ke) seed extract. Int J Food Sci Nutr. 2014;65(5):589–593. https://doi.org/10.3109/09637486.2014.886181
34. Onywere G, Stewart R, Reid S-J, Osbourne J, Jackson D. Efficacy determination of antimicrobial properties in Mentha piperita plant extracts. International Journal of Science & Healthcare Research. 2023;8(2):499-504. https://doi.org/10.52403/ijshr.20230266
35. Sinha S, Aggarwal S, Vijai Singh D. Effl ux pumps: gatekeepers of antibiotic resistance in Staphylococcus aureus biofi lms. Microbial Cell. 2024;11(1):368-377. https://doi.org/10.15698/mic2024.11.839
36. Takó M, Kerekes EB, Zambrano C, Kotogán A, Papp T, Krisch J, et al. Plant phenolics and phenolic-enriched extracts as antimicrobial agents against food-contaminating microorganisms. Antioxidants. 2020;9(2):165. https://doi.org/10.3390/antiox9020165
37. Shamsudin NF, Ahmed QU, Mahmood S, Ali Shah SA, Khatib A, Mukhtar S, et al. Antibacterial effects of flavonoids and their structure-activity relationship study: A comparative interpretation. Molecules. 2022;27(4):1149. https://doi.org/10.3390/molecules27041149
38. Mahizan NA, Yang SK, Moo CL, Song AAL, Chong CM, Chong CW, et al. Terpene derivatives as a potential agent against antimicrobial resistance (AMR) pathogens. Molecules. 2019;24(14):2631. https://doi.org/ 10.3390/molecules24142631
39. Bubonja-Šonje M, Knežević S, Abram M. Challenges to antimicrobial susceptibility testing of plant-derived polyphenolic compounds. Arh Hig Rada Toksikol. 2020;71(4):300–311. https://doi.org/10.2478/aiht-2020-71-3396
40. Masuku M, Mozirandi W, Mukanganyama S. Evaluation of the antibacterial and antibiofilm effects of ethyl acetate root extracts from Vernonia adoensis (Asteraceae) against Pseudomonas aeruginosa. Sci World J. 2023;2023(1):5782656. https://doi.org/ 10.1155/2023/5782656.
41. Famuyide IM, Aro AO, Fasina FO, Eloff JN, McGaw LJ. Antibacterial and antibiofilm activity of acetone leaf extracts of nine under-investigated South African Eugenia and Syzygium (Myrtaceae) species and their selectivity indices. BMC Complement Altern Med. 2019;19(1):141. https://doi.org/10.1186/s12906-019-2547-z

Details

Primary Language

English

Subjects

Pharmacognosy, Pharmaceutical Chemistry

Journal Section

Research Article

Authors

Shu Xian Chong ^*
0009-0004-9152-7090
Malaysia

Lee Huang Chia
0009-0003-9855-0261
Malaysia

Wen Xin Lim
0009-0007-9896-5351
Malaysia

Publication Date

June 1, 2026

Submission Date

August 19, 2025

Acceptance Date

November 7, 2025

Published in Issue

Year 2026 Volume: 46 Number: 2

DOI

https://doi.org/10.52794/hujpharm.1768487

IZ

https://izlik.org/JA54CA54GZ

Cite

RIS / Bibtex

APA

Chong, S. X., Chia, L. H., & Lim, W. X. (2026). Phytochemical Investigation and Antibacterial Activity of Longan Peel Extracts Using Green Solvents. Hacettepe University Journal of the Faculty of Pharmacy, 46(2), 126-136. https://doi.org/10.52794/hujpharm.1768487

AMA

1.Chong SX, Chia LH, Lim WX. Phytochemical Investigation and Antibacterial Activity of Longan Peel Extracts Using Green Solvents. HUJPHARM. 2026;46(2):126-136. doi:10.52794/hujpharm.1768487

Chicago

Chong, Shu Xian, Lee Huang Chia, and Wen Xin Lim. 2026. “Phytochemical Investigation and Antibacterial Activity of Longan Peel Extracts Using Green Solvents”. Hacettepe University Journal of the Faculty of Pharmacy 46 (2): 126-36. https://doi.org/10.52794/hujpharm.1768487.

EndNote

Chong SX, Chia LH, Lim WX (June 1, 2026) Phytochemical Investigation and Antibacterial Activity of Longan Peel Extracts Using Green Solvents. Hacettepe University Journal of the Faculty of Pharmacy 46 2 126–136.

IEEE

[1]S. X. Chong, L. H. Chia, and W. X. Lim, “Phytochemical Investigation and Antibacterial Activity of Longan Peel Extracts Using Green Solvents”, HUJPHARM, vol. 46, no. 2, pp. 126–136, June 2026, doi: 10.52794/hujpharm.1768487.

ISNAD

Chong, Shu Xian - Chia, Lee Huang - Lim, Wen Xin. “Phytochemical Investigation and Antibacterial Activity of Longan Peel Extracts Using Green Solvents”. Hacettepe University Journal of the Faculty of Pharmacy 46/2 (June 1, 2026): 126-136. https://doi.org/10.52794/hujpharm.1768487.

JAMA

1.Chong SX, Chia LH, Lim WX. Phytochemical Investigation and Antibacterial Activity of Longan Peel Extracts Using Green Solvents. HUJPHARM. 2026;46:126–136.

MLA

Chong, Shu Xian, et al. “Phytochemical Investigation and Antibacterial Activity of Longan Peel Extracts Using Green Solvents”. Hacettepe University Journal of the Faculty of Pharmacy, vol. 46, no. 2, June 2026, pp. 126-3, doi:10.52794/hujpharm.1768487.

Vancouver

1.Shu Xian Chong, Lee Huang Chia, Wen Xin Lim. Phytochemical Investigation and Antibacterial Activity of Longan Peel Extracts Using Green Solvents. HUJPHARM. 2026 Jun. 1;46(2):126-3. doi:10.52794/hujpharm.1768487